LOCUS       sequence-347261-        4179 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible gene expression, pACYC origin, LuxR activator, PLuxB 
            promoter, mRFP, Chloramphenicol Resistance Marker. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4179)
  TITLE     Reisch Lab Duet Plasmids
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pALxR1
FEATURES             Location/Qualifiers
     source          1..4179
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(28..46)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      42..89
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(310..969)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     884..903
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(970..1072)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(1464..1481)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1598..2143)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1678..1697)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2368..2384
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_RNA        3362..3411
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             3456..3467
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             3474..4151
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
ORIGIN
        1 gctttccagt cgggaaacct gctgccaccg ctgagcaata actagcataa ccccttgggg
       61 cctctaaacg ggtcttgagg ggttttttgc tgaaacctca ggcatttgag aagcacacgg
      121 tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt
      181 aacgaccctg ccctgaaccg acgaccgggt cgaatttgct ttcgaatttc tgccattcat
      241 ccgcttatta tcacttattc aggcgtagca accaggcgtt taagggcacc aataactgcc
      301 ttaaaaaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat
      361 tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag
      421 caccttgtcg ccttgcgtat aatatttgcc catagtgaaa acgggggcga agaagttgtc
      481 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa
      541 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac
      601 atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga
      661 tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat
      721 caccagctca ccgtctttca ttgccatacg gaactccgga tgagcattca tcaggcgggc
      781 aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa
      841 ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc
      901 ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt
      961 tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg
     1021 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgccgat caacgtctca
     1081 ttttcgccaa aagttggccc agggcttccc ggtatcaaca gggacaccag gatttattta
     1141 ttctgcgaag tgatcttccg tcacaggtat ttattcggcg caaagtgcgt cgggtgatgc
     1201 tgccaactta ctgatttagt gtatgatggt gtttttgagg tgctccagtg gcttctgttt
     1261 ctatcagctg tccctcctgt tcagctactg acggggtggt gcgtaacggc aaaagcaccg
     1321 ccggacatca gcgctagcgg agtgtatact ggcttactat gttggcactg atgagggtgt
     1381 cagtgaagtg cttcatgtgg caggagaaaa aaggctgcac cggtgcgtca gcagaatatg
     1441 tgatacagga tatattccgc ttcctcgctc actgactcgc tacgctcggt cgttcgactg
     1501 cggcgagcgg aaatggctta cgaacggggc ggagatttcc tggaagatgc caggaagata
     1561 cttaacaggg aagtgagagg gccgcggcaa agccgttttt ccataggctc cgcccccctg
     1621 acaagcatca cgaaatctga cgctcaaatc agtggtggcg aaacccgaca ggactataaa
     1681 gataccaggc gtttccccct ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt
     1741 ttaccggtgt cattccgctg ttatggccgc gtttgtctca ttccacgcct gacactcagt
     1801 tccgggtagg cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac
     1861 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca
     1921 ccactggcag cagccactgg taattgattt agaggagtta gtcttgaagt catgcgccgg
     1981 ttaaggctaa actgaaagga caagttttgg tgactgcgct cctccaagcc agttacctcg
     2041 gttcaaagag ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg cggttttttc
     2101 gttttcagag caagagatta cgcgcagacc aaaacgatct caagaagatc atcttattaa
     2161 tcagataaaa tatttctaga tttcagtgca atttatctct tcaaatgtag cacctgaagt
     2221 catccccata cgatataagt tgtaattctc atgttagtca tgccccgcgc ccaccggaag
     2281 gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg
     2341 agctaactta cattaattgc gttgcgcgta aaacgacggc cagtgatcaa tttttaaagt
     2401 atgggcaatc aattgctcct gttaaaattg ctttagaaat actttggcag cggtttgttg
     2461 tattgagttt catttgcgca ttggttaaat ggaaagtgac agtacgctca ctgcaaccta
     2521 atatttttga aatatcccaa gagctttttc cttcgcatgc ccacgctaaa cattcttttt
     2581 ctcttttggt taaatcgttg tttgatttat tatttgctat atttattttt cgataattat
     2641 caactagaga aggaacaatt aatggtatgt tcatacacgc atgtaaaaat aaactatcta
     2701 tatagttgtc tttttctgaa tgtgcaaaac taagcattcc gaagccattg ttagccgtat
     2761 gaatagggaa actaaaccca gtgataagac ctgatgtttt cgcttcttta attacatttg
     2821 gagatttttt atttacagca ttgttttcaa atatattcca attaattggt gaatgattgg
     2881 agttagaata atctactata ggatcatatt ttatcaaatt agcgtcatca taatattgcc
     2941 tccatttttt agggtaatta tctaggattg aaatatcaga tttaaccata gaatgaggat
     3001 aaatgatcgc gagtaaataa tattcacaat gtaccatttt agtcatatca gataagcatt
     3061 gattaatatc attattgctt ctacaagctt taattttatt aattattctg tatgtgtcgt
     3121 cggcatttat gtttttcatt ctgcccctcc gaaaggctta agtcctcgcg tagtacctgg
     3181 gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggcgcgcc gcccaattat
     3241 tgaaggcctc cctaacgggg ggcctttttt tgtttctggt ctcccgctta acgatcgttg
     3301 gctgacctgt aggatcgtac aggtttacgc aagaaaatgg tttgttacag tcgaataaaa
     3361 gctgtcaccg gatgtgcttt ccggtctgat gagtccgtga ggacgaaaca gcctctacaa
     3421 ataattttgt ttaagaactg ggaagagcgc agattaaaga ggagaaatac tagatggctt
     3481 cctccgaaga cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa ggttccgtta
     3541 acggtcacga gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa ggtacccaga
     3601 ccgctaaact gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac atcctgtccc
     3661 cgcagttcca gtacggttcc aaagcttacg ttaaacaccc ggctgacatc ccggactacc
     3721 tgaaactgtc cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc gaagacggtg
     3781 gtgttgttac cgttacccag gactcctccc tgcaagacgg tgagttcatc tacaaagtta
     3841 aactgcgtgg taccaacttc ccgtccgacg gtccggttat gcagaaaaaa accatgggtt
     3901 gggaagcttc caccgaacgt atgtacccgg aagacggtgc tctgaaaggt gaaatcaaaa
     3961 tgcgtctgaa actgaaagac ggtggtcact acgacgctga agttaaaacc acctacatgg
     4021 ctaaaaaacc ggttcagctg ccgggtgctt acaaaaccga catcaaactg gacatcacct
     4081 cccacaacga agactacacc atcgttgaac agtacgaacg tgctgaaggt cgtcactcca
     4141 ccggtgctta ataacgctga tagtgctagt gtagatcgc
//
