LOCUS       sequence-347274-       10395 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible overexpression of GATA6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10395)
  AUTHORS   Romero-Tejeda M, Fonoudi H, Weddle CJ, DeKeyser JM, Lenny B, 
            Fetterman KA, Magdy T, Sapkota Y, Epting C, Burridge PW
  TITLE     A novel transcription factor combination for direct reprogramming to
            a spontaneously contracting human cardiomyocyte-like state.
  JOURNAL   J Mol Cell Cardiol. 2023 Jul 6:S0022-2828(23)00106-2. doi: 
            10.1016/j.yjmcc.2023.06.005.
  PUBMED    37421991
REFERENCE   2  (bases 1 to 10395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Cell 
            Cardiol. 2023 Jul 6:S0022-2828(23)00106-2. doi: 
            10.1016/j.yjmcc.2023.06.005."
COMMENT     Sequence Label: tetO-GATA6
FEATURES             Location/Qualifiers
     source          1..10395
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(49..68)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        240..619
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        621..819
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             837..1017
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1064..1189
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1682..1915
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2100..2144
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2442..2559
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2612..2630,2631..2653,2654..2672,2673..2695,2696..2714,
                     2715..2737,2738..2756,2757..2779,2780..2798,2799..2821,
                     2822..2840,2841..2863,2864..2882)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2915..2953
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2950..2974
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     3053..3072
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3053..3071
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3103..3127
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4101..4130
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     protein_bind    complement(4921..4945)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4971..5012
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5022..5039
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    5089..5677
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5142..5162)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5560..5571)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5680..5696)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5681..5697)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6202..6382
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6408..6425)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6414..6638
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6684..7112
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6771..6790)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6981..7002
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7121..7141)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7126..7455
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7306..7441
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7368..7387
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7503..7550
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7569..7943
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8073..8194
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8110..8129)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8164..8183
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8243..8259)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8243..8259)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8256..8278)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8267..8283
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8291..8297,8298..8315,8316..8321))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8336..8357
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8474..8491)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8645..9233)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8725..8744)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9404..10195,10196..10264))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10027..10046
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10265..10369)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc
       61 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
      121 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
      181 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg
      241 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
      301 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      361 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      421 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      481 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      541 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      601 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      661 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      721 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      781 gggcggtagg cgtgtacggt gggaggtcta tataagcagc gcgttttgcc tgtactgggt
      841 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
      901 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
      961 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     1021 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     1081 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     1141 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     1201 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     1261 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     1321 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     1381 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     1441 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     1501 caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata
     1561 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
     1621 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
     1681 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
     1741 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
     1801 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
     1861 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
     1921 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
     1981 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
     2041 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
     2101 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
     2161 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
     2221 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
     2281 tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag
     2341 aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga tcggcactgc
     2401 gtgcgccaat tctgcagaca aatggcagta ttcatccaca attttaaaag aaaagggggg
     2461 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
     2521 aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc
     2581 agagatccag tttggttatc gagtttacca ctccctatca gtgatagaga aaagtgaaag
     2641 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct
     2701 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
     2761 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
     2821 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
     2881 gaaaagtgaa agtcgagctc ggtacccggg tcgaggtagg cgtgtacggt gggaggccta
     2941 tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt
     3001 tgacctccat agaagacacc gggaccgatc cagcctccgc ggccccgaat tgtaatacga
     3061 ctcactatag ggagacccaa gctggctagg taagcttgat caacaagttt gtacaaaaaa
     3121 gcaggctcac catggccttg actgacggcg gctggtgctt gccgaagcgc ttcggggccg
     3181 cgggtgcgga cgccagcgac tccagagcct ttccagcgcg ggagccctcc acgccgcctt
     3241 cccccatctc ttcctcgtcc tcctcctgct cccggggcgg agagcggggc cccggcggcg
     3301 ccagcaactg cgggacgcct cagctcgaca cggaggcggc ggccggaccc ccggcccgct
     3361 cgctgctgct cagttcctac gcttcgcatc ccttcggggc tccccacgga ccttcggcgc
     3421 ctggggtcgc gggccccggg ggcaacctgt cgagctggga ggacttgctg ctgttcactg
     3481 acctcgacca agccgcgacc gccagcaagc tgctgtggtc cagccgcggc gccaagctga
     3541 gccccttcgc acccgagcag ccggaggaga tgtaccagac cctcgccgct ctctccagcc
     3601 agggtccggc cgcctacgac ggcgcgcccg gcggcttcgt gcactctgcg gccgcggcgg
     3661 cagcagccgc ggcggcggcc agctccccgg tctacgtgcc caccacccgc gtgggttcca
     3721 tgctgcccgg cctaccgtac cacctgcagg ggtcgggcag tgggccagcc aaccacgcgg
     3781 gcggcgcggg cgcgcacccc ggctggcctc aggcctcggc cgacagccct ccatacggca
     3841 gcggaggcgg cgcggctggc ggcggggccg cggggcctgg cggcgctggc tcagccgcgg
     3901 cgcacgtctc ggcgcgcttc ccctactctc ccagcccgcc catggccaac ggcgccgcgc
     3961 gggagccggg aggctacgcg gcggcgggca gtgggggcgc gggaggcgtg agcggcggcg
     4021 gcagtagcct ggcggccatg ggcggccgcg agccccagta cagctcgctg tcggccgcgc
     4081 ggccgctgaa cgggacgtac caccaccacc accaccacca ccaccaccat ccgagcccct
     4141 actcgcccta cgtgggggcg ccactgacgc ctgcctggcc cgccggaccc ttcgagaccc
     4201 cggtgctgca cagcctgcag agccgcgccg gagccccgct cccggtgccc cggggtccca
     4261 gtgcagacct gctggaggac ctgtccgaga gccgcgagtg cgtgaactgc ggctccatcc
     4321 agacgccgct gtggcggcgg gacggcaccg gccactacct gtgcaacgcc tgcgggctct
     4381 acagcaagat gaacggcctc agccggcccc tcatcaagcc gcagaagcgc gtgccttcat
     4441 cacggcggct tggattgtcc tgtgccaact gtcacaccac aactaccacc ttatggcgca
     4501 gaaacgccga gggtgaaccc gtgtgcaatg cttgtggact ctacatgaaa ctccatgggg
     4561 tgcccagacc acttgctatg aaaaaagagg gaattcaaac caggaaacga aaacctaaga
     4621 acataaataa atcaaagact tgctctggta atagcaataa ttccattccc atgactccaa
     4681 cttccacctc ttctaactca gatgattgca gcaaaaatac ttcccccaca acacaaccta
     4741 cagcctcagg ggcgggtgcc ccggtgatga ctggtgcggg agagagcacc aatcccgaga
     4801 acagcgagct caagtattcg ggtcaagatg ggctctacat aggcgtcagt ctcgcctcgc
     4861 cggccgaagt cacgtcctcc gtgcgaccgg attcctggtg cgccctggcc ctggcctgaa
     4921 acccagcttt cttgtacaaa gtggttgatc tagagggccc gcggttcgaa ggtaagccta
     4981 tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg tcatcatcac catcaccatt
     5041 gagtttaaac ccgctgatca gcctcaattc gatatcaagc ttatcgataa tcaacctctg
     5101 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     5161 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     5221 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     5281 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     5341 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     5401 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     5461 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     5521 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     5581 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     5641 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgagac
     5701 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc
     5761 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta
     5821 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     5881 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac
     5941 acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca
     6001 ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc
     6061 aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg
     6121 gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca catggcccga
     6181 gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     6241 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     6301 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     6361 tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     6421 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     6481 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     6541 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     6601 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     6661 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     6721 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     6781 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     6841 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     6901 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     6961 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     7021 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     7081 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     7141 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     7201 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     7261 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     7321 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     7381 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     7441 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac
     7501 gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga
     7561 actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg
     7621 gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact
     7681 tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg
     7741 tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt
     7801 ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg
     7861 gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact
     7921 tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct
     7981 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     8041 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
     8101 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     8161 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
     8221 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     8281 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     8341 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     8401 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     8461 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     8521 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     8581 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     8641 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     8701 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     8761 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     8821 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     8881 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     8941 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     9001 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     9061 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     9121 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     9181 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     9241 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     9301 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     9361 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     9421 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     9481 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     9541 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     9601 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     9661 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     9721 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     9781 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     9841 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     9901 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     9961 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
    10021 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
    10081 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
    10141 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
    10201 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
    10261 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    10321 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    10381 gaaaagtgcc acctg
//
