LOCUS       sequence-347278-        9622 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible overexpression of HEY2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9622)
  AUTHORS   Romero-Tejeda M, Fonoudi H, Weddle CJ, DeKeyser JM, Lenny B, 
            Fetterman KA, Magdy T, Sapkota Y, Epting C, Burridge PW
  TITLE     A novel transcription factor combination for direct reprogramming to
            a spontaneously contracting human cardiomyocyte-like state.
  JOURNAL   J Mol Cell Cardiol. 2023 Jul 6:S0022-2828(23)00106-2. doi: 
            10.1016/j.yjmcc.2023.06.005.
  PUBMED    37421991
REFERENCE   2  (bases 1 to 9622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Cell 
            Cardiol. 2023 Jul 6:S0022-2828(23)00106-2. doi: 
            10.1016/j.yjmcc.2023.06.005."
COMMENT     Sequence Label: tetO-HEY2
FEATURES             Location/Qualifiers
     source          1..9622
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(49..68)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        240..619
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        621..819
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             837..1017
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1064..1189
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1682..1915
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2100..2144
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2442..2559
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2612..2630,2631..2653,2654..2672,2673..2695,2696..2714,
                     2715..2737,2738..2756,2757..2779,2780..2798,2799..2821,
                     2822..2840,2841..2863,2864..2882)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2915..2953
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2950..2974
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     3053..3072
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3053..3071
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3103..3127
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4148..4172)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4198..4239
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4249..4266
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    4316..4904
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4369..4389)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4787..4798)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4907..4923)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4908..4924)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5429..5609
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5635..5652)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5641..5865
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5911..6339
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5998..6017)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6208..6229
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6348..6368)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6353..6682
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6533..6668
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6595..6614
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6730..6777
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6796..7170
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7300..7421
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7337..7356)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7391..7410
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7470..7486)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7470..7486)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7483..7505)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7494..7510
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7518..7524,7525..7542,7543..7548))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7563..7584
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7701..7718)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7872..8460)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7952..7971)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8631..9422,9423..9491))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9254..9273
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9492..9596)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc
       61 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
      121 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
      181 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg
      241 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
      301 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      361 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      421 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      481 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      541 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      601 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      661 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      721 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      781 gggcggtagg cgtgtacggt gggaggtcta tataagcagc gcgttttgcc tgtactgggt
      841 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
      901 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
      961 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     1021 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     1081 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     1141 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     1201 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     1261 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     1321 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     1381 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     1441 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     1501 caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata
     1561 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
     1621 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
     1681 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
     1741 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
     1801 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
     1861 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
     1921 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
     1981 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
     2041 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
     2101 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
     2161 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
     2221 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
     2281 tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag
     2341 aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga tcggcactgc
     2401 gtgcgccaat tctgcagaca aatggcagta ttcatccaca attttaaaag aaaagggggg
     2461 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
     2521 aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc
     2581 agagatccag tttggttatc gagtttacca ctccctatca gtgatagaga aaagtgaaag
     2641 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct
     2701 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
     2761 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
     2821 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
     2881 gaaaagtgaa agtcgagctc ggtacccggg tcgaggtagg cgtgtacggt gggaggccta
     2941 tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt
     3001 tgacctccat agaagacacc gggaccgatc cagcctccgc ggccccgaat tgtaatacga
     3061 ctcactatag ggagacccaa gctggctagg taagcttgat caacaagttt gtacaaaaaa
     3121 gcaggcttca ccatgaagcg cccctgcgag gagacgacct ccgagagcga catggacgag
     3181 accatcgacg tggggagcga gaacaattac tcggggcaaa gtactagctc tgtgattaga
     3241 ttgaattctc caacaacaac atctcagatt atggcaagaa agaaaaggag agggattata
     3301 gagaaaaggc gtcgggatcg gataaataac agtttatctg agttgagaag acttgtgcca
     3361 actgcttttg aaaaacaagg atctgcaaag ttagaaaaag ctgaaatatt gcaaatgaca
     3421 gtggatcatt tgaagatgct tcaggcaaca gggggtaaag gctactttga cgcacacgct
     3481 cttgccatgg acttcatgag cataggattc cgagagtgcc taacagaagt tgcgcggtac
     3541 ctgagctccg tggaaggcct ggactcctcg gatccgctgc gggtgcggct tgtgtctcat
     3601 ctcagcactt gcgccaccca gcgggaggcg gcggccatga catcctccat ggcccaccac
     3661 catcatccgc tccacccgca tcactgggcc gccgccttcc accacctgcc cgcagccctg
     3721 ctccagccca acggcctcca tgcctcagag tcaacccctt gtcgcctctc cacaacttca
     3781 gaagtgcctc ctgcccacgg ctctgctctc ctcacggcca cgtttgccca tgcggattca
     3841 gccctccgaa tgccatccac gggcagcgtc gccccctgcg tgccacctct ctccacctct
     3901 ctcttgtccc tctctgccac cgtccacgcc gcagccgcag cagccaccgc ggctgcacac
     3961 agcttccctc tgtccttcgc gggggcattc cccatgcttc ccccaaacgc agcagcagca
     4021 gtggccgcgg ccacagccat cagcccgccc ttgtcagtat cagccacgtc cagtcctcag
     4081 cagaccagca gtggaacaaa caataaacct taccgaccct gggggacaga agttggagct
     4141 ttttaaaacc cagctttctt gtacaaagtg gttgatctag agggcccgcg gttcgaaggt
     4201 aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtca tcatcaccat
     4261 caccattgag tttaaacccg ctgatcagcc tcaattcgat atcaagctta tcgataatca
     4321 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     4381 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     4441 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     4501 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     4561 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     4621 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     4681 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     4741 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     4801 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     4861 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc
     4921 tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga
     4981 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt
     5041 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     5101 ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat
     5161 ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc cagggatcag
     5221 atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag agaaggtaga
     5281 agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga
     5341 tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat
     5401 ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg
     5461 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     5521 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     5581 cttttagtca gtgtggaaaa tctctagcag ggcccgttta aacccgctga tcagcctcga
     5641 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     5701 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     5761 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     5821 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     5881 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     5941 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     6001 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     6061 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     6121 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     6181 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     6241 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     6301 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     6361 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     6421 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     6481 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     6541 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     6601 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     6661 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     6721 tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag
     6781 gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac
     6841 gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag
     6901 gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac
     6961 caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac
     7021 gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc
     7081 gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc
     7141 gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc
     7201 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     7261 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     7321 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     7381 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     7441 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     7501 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     7561 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     7621 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     7681 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     7741 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     7801 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     7861 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     7921 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     7981 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8041 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     8101 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     8161 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8221 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8281 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     8341 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     8401 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     8461 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     8521 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     8581 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     8641 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     8701 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     8761 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     8821 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     8881 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     8941 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     9001 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     9061 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     9121 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     9181 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     9241 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     9301 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     9361 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     9421 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     9481 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     9541 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     9601 tttccccgaa aagtgccacc tg
//
