LOCUS       sequence-347281-       10060 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible overexpression of IRX5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Romero-Tejeda M, Fonoudi H, Weddle CJ, DeKeyser JM, Lenny B, 
            Fetterman KA, Magdy T, Sapkota Y, Epting C, Burridge PW
  TITLE     A novel transcription factor combination for direct reprogramming to
            a spontaneously contracting human cardiomyocyte-like state.
  JOURNAL   J Mol Cell Cardiol. 2023 Jul 6:S0022-2828(23)00106-2. doi: 
            10.1016/j.yjmcc.2023.06.005.
  PUBMED    37421991
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Cell 
            Cardiol. 2023 Jul 6:S0022-2828(23)00106-2. doi: 
            10.1016/j.yjmcc.2023.06.005."
COMMENT     Sequence Label: tetO-IRX5
FEATURES             Location/Qualifiers
     source          1..10060
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        287..325
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     322..346
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     425..444
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        425..443
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    475..499
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(1720..1731)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(1958..1982)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2008..2049
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2059..2076
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    2126..2714
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2179..2199)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2597..2608)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2717..2733)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2718..2734)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3239..3419
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3445..3462)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3451..3675
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3721..4149
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3808..3827)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4018..4039
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4158..4178)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4163..4492
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4343..4478
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4405..4424
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4540..4587
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4606..4980
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5110..5231
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5147..5166)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5201..5220
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5280..5296)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5280..5296)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5293..5315)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5304..5320
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5328..5334,5335..5352,5353..5358))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5373..5394
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5511..5528)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5682..6270)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5762..5781)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6441..7232,7233..7301))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7064..7083
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7302..7406)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7481..7500)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7672..8051
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8053..8251
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             8269..8449
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8496..8621
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9114..9347
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9532..9576
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9874..9991
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(10044..10060,1..2,3..25,26..44,45..67,68..86,87..109,
                     110..128,129..151,152..170,171..193,194..212,213..235,
                     236..254)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
ORIGIN
        1 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
       61 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
      121 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
      181 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct
      241 atcagtgata gagaaaagtg aaagtcgagc tcggtacccg ggtcgaggta ggcgtgtacg
      301 gtgggaggcc tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca
      361 tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc gcggccccga
      421 attgtaatac gactcactat agggagaccc aagctggcta ggtaagcttg atcaacaagt
      481 ttgtacaaaa aagcaggctt caccatgtcc tatccgcagg gctacttgta ccagccgtcc
      541 gcctcgctgg cgctctactc gtgcccggcg tacagcacca gcgtcatttc ggggccccgc
      601 acggatgagc tcggccgctc ttcttcgggc tccgcgttct cgccctacgc tggctcgact
      661 gccttcacgg cgccctcgcc gggctacaac tcgcacctcc agtacggcgc cgaccccgcg
      721 gccgccgccg ccgccgcctt ctcctcgtac gtgggctctc cctacgacca cacacccggc
      781 atggcgggct ccttggggta ccatccttac gcggcgcccc tgggatcgta cccttacggg
      841 gacccagcgt accggaagaa cgccacaagg gacgccacgg ctaccctcaa ggcctggctc
      901 aacgagcacc gcaagaaccc ctaccccacc aagggcgaga agatcatgct ggccatcatc
      961 accaagatga ccctcaccca ggtgtccacc tggttcgcca acgcgcgccg gcgcctcaag
     1021 aaagagaata aaatgacgtg gacgccgcgg aaccgcagcg aggacgagga agaggaggag
     1081 aacattgacc tggagaagaa cgacgaggac gagccccaga agcccgagga caagggcgac
     1141 cccgagggcc ccgaagcagg aggagctgag cagaaggcgg cttcgggctg cgaacggctt
     1201 cagggaccac ccacccctgc aggcaaggag acggagggca gcctcagcga ctcggatttt
     1261 aaggagccgc cctcggaggg ccgcctcgac gcgctgcagg gccccccccg caccggcggg
     1321 ccctccccgg ctgggccagc ggcggcgcgg ctggcggagg acccggcccc tcactacccc
     1381 gccggagcgc cggcgcccgg cccgcatcca gccgcgggcg aggtgcctcc gggtcccggc
     1441 gggccctcgg ttatccattc gccgcctccg ccgccgcctc ctgcggtgct cgccaagccc
     1501 aaactgtggt ctttggcaga gatcgccaca tcgtcggaca aggtcaagga cgggggcggc
     1561 gggaacgagg gctctccatg cccaccgtgt cccgggccca tagccgggca agccctagga
     1621 ggcagccggg cgtcgccggc cccggcgccg tcacgctcgc cctcggcgca gtgtcctttt
     1681 ccaggcggga cggtgctgtc ccggcctctc tactacaccg cgcccttcta tcccggctac
     1741 acgaactatg gctccttcgg acaccttcat ggccacccgg ggcccgggcc aggccccaca
     1801 accggtccgg ggtctcattt caatggatta aaccagaccg tgttgaaccg agcggacgct
     1861 ttggctaaag acccgaaaat gttgcggagc cagtctcagc tagacctgtg caaagactct
     1921 ccctatgaat tgaagaaagg tatgtccgac atttaaaacc cagctttctt gtacaaagtg
     1981 gttgatctag agggcccgcg gttcgaaggt aagcctatcc ctaaccctct cctcggtctc
     2041 gattctacgc gtaccggtca tcatcaccat caccattgag tttaaacccg ctgatcagcc
     2101 tcaattcgat atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt
     2161 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     2221 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     2281 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     2341 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     2401 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     2461 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     2521 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     2581 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     2641 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     2701 ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg gagcaatcac
     2761 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga
     2821 ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc
     2881 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     2941 acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg
     3001 gcagaactac acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa
     3061 gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt
     3121 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag
     3181 gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg
     3241 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     3301 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     3361 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     3421 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     3481 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     3541 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     3601 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     3661 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     3721 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     3781 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     3841 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     3901 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     3961 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     4021 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     4081 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     4141 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     4201 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     4261 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     4321 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     4381 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     4441 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     4501 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca
     4561 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc
     4621 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac
     4681 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac
     4741 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg
     4801 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac
     4861 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag
     4921 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga
     4981 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     5041 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     5101 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     5161 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     5221 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     5281 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     5341 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     5401 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     5461 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     5521 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     5581 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     5641 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     5701 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     5761 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     5821 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     5881 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     5941 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     6001 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     6061 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     6121 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     6181 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     6241 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     6301 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     6361 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     6421 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     6481 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     6541 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     6601 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     6661 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     6721 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     6781 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     6841 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     6901 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     6961 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     7021 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     7081 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     7141 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     7201 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     7261 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     7321 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     7381 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
     7441 ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg
     7501 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga
     7561 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta
     7621 gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat
     7681 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     7741 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     7801 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     7861 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     7921 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     7981 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     8041 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
     8101 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     8161 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
     8221 ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg cctgtactgg gtctctctgg
     8281 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     8341 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     8401 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     8461 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     8521 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     8581 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     8641 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     8701 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     8761 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     8821 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     8881 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     8941 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     9001 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     9061 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     9121 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     9181 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     9241 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     9301 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     9361 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     9421 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     9481 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     9541 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     9601 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     9661 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     9721 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     9781 ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact gcgtgcgcca
     9841 attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
     9901 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     9961 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
    10021 agtttggtta tcgagtttac cactccctat cagtgataga
//
