LOCUS       sequence-347297-        9771 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of SOS1 fused to EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9771)
  AUTHORS   Suzuki S, Nakamura A, Hatano Y, Yoshikawa M, Yoshii T, Sawada S, 
            Atsuta-Tsunoda K, Aoki K, Tsukiji S
  TITLE     A chemogenetic platform for controlling plasma membrane signaling 
            and synthetic signal oscillation.
  JOURNAL   Cell Chem Biol. 2022 Sep 15;29(9):1446-1464.e10. doi: 
            10.1016/j.chembiol.2022.06.005. Epub 2022 Jul 13.
  PUBMED    35835118
REFERENCE   2  (bases 1 to 9771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2022.06.005"; journalName: "Cell Chem Biol"; 
            date: "2022-09-15- 15"; volume: "29"; issue: "9"; pages: 
            "1446-1464.e10"
COMMENT     Sequence Label: pCAGGS-EGFP-SOS1
FEATURES             Location/Qualifiers
     source          1..9771
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..661
                     /label=chicken beta-actin promoter
     intron          662..1670
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1594..1616
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     regulatory      1759..1768
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1765..1767,1768..1770,1771..2481)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1765..1767,1768..1770,1771..2481)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1810..1831)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2071..2090)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2418..2439
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6636..6656)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6636..6654)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6675..6691)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6793..6812)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6858..6913
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6912..6931)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7274..7290)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7298..7314
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7322..7328,7329..7346,7347..7352))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7367..7388
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7447..7642
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7493..7628
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7648..7782
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7685..7704)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7739..7758
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7850..7867)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8021..8609)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8101..8120)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8780..9571,9572..9640))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9403..9422
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9641..9745)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg ggcgagggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tgttgtgctg tctcatcatt ttggcaaaga attcatagta gctagcagtt
     1741 gctcgagagt acccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg
     1801 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     1861 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     1921 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     1981 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     2041 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     2101 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     2161 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     2221 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     2281 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     2341 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     2401 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     2461 ggcatggacg agctgtacaa gtccggactc agatctcgac aaggtagtgg tgctggctct
     2521 ggtgctggta gtggcgctgg ttccggtgct ggctctggcg cgcctcgagc tcaagcttcg
     2581 caggcgcagc agctgcccta cgagtttttc agcgaagaga acgcgcccaa gtggcgggga
     2641 ctactggtgc ctgcgctgaa aaaggtccag gggcaagttc atcctactct cgagtctaat
     2701 gatgatgctc ttcagtatgt tgaagaatta attttgcaat tattaaatat gctatgccaa
     2761 gctcagcccc gaagtgcttc agatgtagag gaacgtgttc aaaaaagttt ccctcatcca
     2821 attgataaat gggcaatagc tgatgcccaa tcagctattg aaaagaggaa gcgaagaaac
     2881 cctttatctc tcccagtaga aaaaattcat cctttattaa aggaggtcct aggttataaa
     2941 attgaccacc aggtttctgt ttacatagta gcagtcttag aatacatttc tgcagacatt
     3001 ttaaagctgg ttgggaatta tgtaagaaat atacggcatt atgaaattac aaaacaagat
     3061 attaaagtgg caatgtgtgc tgacaaggta ttgatggata tgtttcatca agatgtagaa
     3121 gatattaata tattatcttt aactgacgaa gagccttcca cctcaggaga acaaacttac
     3181 tatgatttgg taaaagcatt tatggcagaa attcgacaat atataaggga actaaatcta
     3241 attataaaag tttttagaga gccctttgtc tccaattcaa aattgttttc agctaatgat
     3301 gtagaaaata tatttagtcg catagtagat atacatgaac ttagtgtaaa gttactgggc
     3361 catatagaag atacagtaga aatgacagat gaaggcagtc cccatccact agtaggaagc
     3421 tgctttgaag acttagcaga ggaactggca tttgatccat atgaatcgta tgctcgagat
     3481 attttgcgac ctggttttca tgatcgtttc cttagtcagt tatcaaagcc tggggcagca
     3541 ctttatttgc agtcaatagg cgaaggtttc aaagaagctg ttcaatatgt tttacccagg
     3601 ctgcttctgg cccctgttta ccactgtctc cattactttg aacttttgaa gcagttagaa
     3661 gaaaaaagtg aagatcaaga agacaaggaa tgtttaaaac aagcaataac agctttgctt
     3721 aatgttcaga gtggtatgga aaaaatatgt tctaaaagtc ttgcaaaacg aagactgagt
     3781 gaatctgcat gtcggtttta tagtcagcaa atgaagggga aacaactagc aatcaagaag
     3841 atgaacgaga ttcagaagaa tattgatggt tgggagggaa aagacattgg acagtgttgt
     3901 aatgaattta taatggaagg aactcttaca cgtgtaggag ccaaacatga gagacacata
     3961 tttctctttg atggcttaat gatttgctgt aaatcaaatc atgggcagcc aagacttcct
     4021 ggtgctagca atgcagaata tcgtcttaaa gaaaagtttt ttatgcgaaa ggtacaaatt
     4081 aatgataaag atgacaccaa tgaatacaag catgcttttg aaataatttt aaaagatgaa
     4141 aatagtgtta tattttctgc caagtcagct gaagagaaaa acaattggat ggcagcattg
     4201 atatctttac agtaccggag tacactggaa aggatgcttg atgtaacaat gctacaggaa
     4261 gagaaagagg agcagatgag gctgcctagt gctgatgttt atagatttgc agagcctgac
     4321 tctgaagaga atattatatt tgaagagaac atgcagccca aggctggaat tccaattatc
     4381 aaagcaggaa ctgttattaa acttatagag aggcttacgt accatatgta cgcagatccc
     4441 aattttgttc ggacatttct tacaacatac agatcctttt gcaaacctca agaactactg
     4501 agtcttataa tagaaaggtt tgaaattcca gagcctgagc caacagaagc tgatcgcata
     4561 gctatagaga atggagatca acccttgagt gcagaactga aaagatttag aaaagaatat
     4621 atacagcctg tgcaactgcg agtattaaat gtatgtcggc actgggtaga gcaccacttc
     4681 tatgattttg aaagagatgc atatcttttg caacgaatgg aagaatttat tggaacagta
     4741 agaggtaaag caatgaaaaa atgggttgaa tccatcacta aaataatcca aaggaaaaaa
     4801 attgcaagag acaatggacc aggtcataat attacatttc agagttcacc tcccacagtt
     4861 gagtggcata taagcagacc tgggcacata gagacttttg acctgctcac cttacaccca
     4921 atagaaattg ctcgacaact cactttactt gaatcagatc tataccgagc tgtacagcca
     4981 tcagaattag ttggaagtgt gtggacaaaa gaagacaaag aaattaactc tcctaatctt
     5041 ctgaaaatga ttcgacatac caccaacctc actctgtggt ttgagaaatg tattgtagaa
     5101 actgaaaatt tagaagaaag agtagctgtg gtgagtcgaa ttattgagat tctacaagtc
     5161 tttcaagagt tgaacaactt taatggtgtc cttgaggttg tcagtgctat gaattcatca
     5221 cctgtttaca gactagacca cacatttgag caaataccaa gtcgccagaa gaaaatttta
     5281 gaagaagctc atgaattgag tgaagatcac tataagaaat atttggcaaa actcaggtct
     5341 attaatccac catgtgtgcc tttctttgga atttatctca ctaatatctt gaaaacagaa
     5401 gaaggcaacc ctgaggtcct aaaaagacat ggaaaagagc ttataaactt tagcaaaagg
     5461 aggaaagtag cagaaataac aggagagatc cagcagtacc aaaatcagcc ttactgttta
     5521 cgagtagaat cagatatcaa aaggttcttt gaaaacttga atccgatggg aaatagcatg
     5581 gagaaggaat ttacagatta tcttttcaac aaatccctag aaatagaacc acgaaaccct
     5641 aagcctctcc caagatttcc aaaaaaatat agctatcccc taaaatctcc tggtgttcgt
     5701 ccatcaaacc caagaccagg taccatgagg catcccacac ctctgcagca ggagccaagg
     5761 aaaattagtt atagtaggat ccctgaaagt gaaacagaaa gtacagcatc tgcaccaaat
     5821 tctccaagaa caccgttaac acctccgcct gcttctggtg cttccagtac cacagatgtt
     5881 tgcagtgtat ttgattccga tcattcgagc ccttttcact caagcaatga taccgtcttt
     5941 atccaagtta ctctgcccca tggcccaaga tctgcttctg tatcatctat aagtttaacc
     6001 aaaggcactg atgaagtgcc tgtccctcct cctgttcctc cacgaagacg accagaatct
     6061 gccccagcag aatcttcacc atctaagatt atgtctaagc atttggacag tcccccagcc
     6121 attcctccta ggcaacccac atcaaaagcc tattcaccac gatattcaat atcagaccgg
     6181 acctctatct cagaccctcc tgaaagccct cccttattac caccacgaga acctgtgagg
     6241 acacctgatg ttttctcaag ctcaccacta catctccaac ctcccccttt gggcaaaaaa
     6301 agtgaccatg gcaatgcctt cttcccaaac agcccttccc cctttacacc acctcctcct
     6361 caaacacctt ctcctcacgg cacaagaagg catctgccat caccaccatt gacacaagaa
     6421 gtggaccttc attccattgc tgggccgcct gttcctccac gacaaagcac ttctcaacat
     6481 atccctaaac tccctccaaa aacttacaaa agggagcaca cacacccatc catgcacaga
     6541 gatggaccac cactgttgga gaatgcccat tcttcctaaa ccgcgggccc gggatactcg
     6601 cggccgccac cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc
     6661 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct aattcactcc
     6721 tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa
     6781 ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga
     6841 gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt
     6901 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga
     6961 gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg
     7021 gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag
     7081 aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt
     7141 aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact
     7201 actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct
     7261 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     7321 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     7381 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     7441 ggatccgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     7501 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     7561 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     7621 tggaggccta ggcttttgca aaaagctaac ttgtttattg cagcttataa tggttacaaa
     7681 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     7741 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgctgcat taatgaatcg
     7801 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     7861 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     7921 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     7981 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     8041 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     8101 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     8161 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct
     8221 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     8281 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     8341 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     8401 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     8461 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     8521 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     8581 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     8641 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     8701 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     8761 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     8821 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     8881 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     8941 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     9001 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     9061 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     9121 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     9181 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     9241 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     9301 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     9361 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     9421 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     9481 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     9541 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     9601 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     9661 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     9721 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct g
//
