LOCUS       sequence-34734-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Miller DW, Ahmad R, Hague S, Baptista MJ, Canet-Aviles R, McLendon 
            C, Carter DM, Zhu PP, Stadler J, Chandran J, Klinefelter GR, 
            Blackstone C, Cookson MR
  TITLE     L166P mutant DJ-1, causative for recessive Parkinson's disease, is 
            degraded through the ubiquitin-proteasome system.
  JOURNAL   J Biol Chem. 2003 Sep 19. 278(38):36588-95.
  PUBMED    12851414
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2003 Sep 19. 278(38):36588-95."
COMMENT     Sequence Label: pcDNA3.1/GS-DJ1-R98Q-V5
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3..20)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(30..71)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(704..723)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(705..723)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(768..819)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(797..817)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tcatggtgat ggtgatgatg accggtacgc gtagaatcga gaccgaggag agggttaggg
       61 ataggcttac cttcgaatgg gtgacctcga agctcgccct tgtctttaag aacaagtgga
      121 gccttcactt gagccgccac ctccttgcca ttcagggctt caacaattgc aagcgcaaac
      181 tcgaagctgg tcccaggccc ccggcttgta agaatcaggc cgtctttttc cacacgattc
      241 tcagagtagg tgtaatgacc tccattcatc attttgtctt tagcaagagg gtgtgttgta
      301 actttacttc caaaacctat ttcatgagcc aacagagcag taggacctgc acagatggcg
      361 gctatcaggc ccttctggtt ttcctgctcc ttcagtatct ccttcacagc agcagactca
      421 gataaattct gtgcgcccag attacctcct ggtagaacca ccacatcata tggtccctct
      481 ttttttgcat cttcaaggct ggcatcagga caaatgacca catcacggct acactgtact
      541 gggtcttttc cagccaggcc tgcaacggtg accttaatcc cagctcgcct catgacatct
      601 acagggatga ccgtctccat ttcctctgct cctttagcca ggatgaccag agctcttttg
      661 gaagccatgg tgaagggcga gcttaactag ccagcttggg tctccctata gtgagtcgta
      721 ttaatttcga taagccagta agcagtgggt tctctagtta gccagagagc tctgcttata
      781 tagacctccc accgtacacg cctaccgccc atttgcgtca atgg
//
