LOCUS       sequence-34736-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Miller DW, Ahmad R, Hague S, Baptista MJ, Canet-Aviles R, McLendon 
            C, Carter DM, Zhu PP, Stadler J, Chandran J, Klinefelter GR, 
            Blackstone C, Cookson MR
  TITLE     L166P mutant DJ-1, causative for recessive Parkinson's disease, is 
            degraded through the ubiquitin-proteasome system.
  JOURNAL   J Biol Chem. 2003 Sep 19. 278(38):36588-95.
  PUBMED    12851414
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2003 Sep 19. 278(38):36588-95."
COMMENT     Sequence Label: pcDNA3.1/GS-DJ1-E18Q-V5
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..18)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(28..69)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(702..721)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(703..721)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(766..817)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(795..815)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 atggtgatgg tgatgatgac cggttcacgt agaatcgaga ccgaggagag ggttagggat
       61 aggcttacct tcgaatgggt gacctcgaag ctcgcccttg tctttaagaa caagtggagc
      121 cttcacttga gccgccacct ccttgccatt cagggcttca acaattgcaa gcgcaaactc
      181 gaagctggtc ccaggccccc ggcttgtaag aatcaggccg tctttttcca cacgattctc
      241 agagtaggtg taatgacctc cattcatcat tttgtcttta gcaagagggt gtgttgtaac
      301 tttacttcca aaacctattt catgagccaa cagagcagta ggacctgcac agatggcggc
      361 tatcaggccc ttccggtttt cctgctcctt cagtatctcc ttcacagcag cagactcaga
      421 taaattctgt gcgcccagat tacctcctgg tagaaccacc acatcatatg gtccctcttt
      481 ttttgcatct tcaaggctgg catcaggaca aatgaccaca tcacggctac actgtactgg
      541 gtcttttcca gccaggcctg caacggtgac cttaatccca gctcgcctca tgacatctac
      601 agggatgacc gtctgcattt cctctgctcc tttagccagg atgaccagag ctcttttgga
      661 agccatggtg aagggcgagc ttaactagcc agcttgggtc tccctatagt gagtcgtatt
      721 aatttcgata agccagtaag cagtgggttc tctagttagc cagagagctc tgcttatata
      781 gacctcccac cgtacacgcc taccgcccat ttgcgtcaat
//
