LOCUS       sequence-34739-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Miller DW, Ahmad R, Hague S, Baptista MJ, Canet-Aviles R, McLendon 
            C, Carter DM, Zhu PP, Stadler J, Chandran J, Klinefelter GR, 
            Blackstone C, Cookson MR
  TITLE     L166P mutant DJ-1, causative for recessive Parkinson's disease, is 
            degraded through the ubiquitin-proteasome system.
  JOURNAL   J Biol Chem. 2003 Sep 19. 278(38):36588-95.
  PUBMED    12851414
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2003 Sep 19. 278(38):36588-95."
COMMENT     Sequence Label: pcDNA3.1/GS-DJ1-D149A-V5
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(2..19)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(29..70)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(703..722)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(704..722)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(767..818)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(796..816)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 catggtgatg gtgatgatga ccggtacgcg tagaatcgag accgaggaga gggttaggga
       61 taggcttacc ttcgaatggg tgacctcgaa gctcgccctt gtctttaaga acaagtggag
      121 ccttcacttg agccgccacc tccttgccat tcagggcttc aacaattgca agcgcaaact
      181 cgaagctggt cccaggcccc cggcttgtaa gaatcaggcc ggctttttcc acacgattct
      241 cagagtaggt gtaatgacct ccattcatca ttttgtcttt agcaagaggg tgtgttgtaa
      301 ctttacttcc aaaacctatt tcatgagcca acagagcagt aggacctgca cagatggcgg
      361 ctatcaggcc cttccggttt tcctgctcct tcagtatctc cttcacagca gcagactcag
      421 ataaattctg tgcgcccaga ttacctcctg gtagaaccac cacatcatat ggtccctctt
      481 tttttgcatc ttcaaggctg gcatcaggac aaatgaccac atcacggcta cactgtactg
      541 ggtcttttcc agccaggcct gcaacggtga ccttaatccc agctcgcctc atgacatcta
      601 cagggatgac cgtctccatt tcctctgctc ctttagccag gatgaccaga gctcttttgg
      661 aagccatggt gaagggcgag cttaactagc cagcttgggt ctccctatag tgagtcgtat
      721 taatttcgat aagccagtaa gcagtgggtt ctctagttag ccagagagct ctgcttatat
      781 agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcg
//
