LOCUS       sequence-347416-        8407 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of human Alpha7 (CHRNA7) with YFP fused into 
            M3-M4 loop.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8407)
  AUTHORS   Wang Y, Xiao C, Indersmitten T, Freedman R, Leonard S, Lester HA
  TITLE     The duplicated alpha7 subunits assemble and form functional 
            nicotinic receptors with the full-length alpha7.
  JOURNAL   J Biol Chem. 2014 Sep 19;289(38):26451-63. doi: 
            10.1074/jbc.M114.582858. Epub 2014 Jul 23.
  PUBMED    25056953
REFERENCE   2  (bases 1 to 8407)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.582858"; journalName: "J Biol Chem"; date: 
            "2014-09-19- 19"; volume: "289"; issue: "38"; pages: "26451-63"
COMMENT     Sequence Label: pcDNA3.1-CHRNA7-YFP
FEATURES             Location/Qualifiers
     source          1..8407
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(2020..2022,2023..2025,2026..2736)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2065..2086)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2326..2345)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2673..2694
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3595..3612)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3601..3825
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3871..4299
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3958..3977)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4168..4189
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4308..4328)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4313..4642
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4493..4628
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4555..4574
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4691..5716
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             4691..5716
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    5846..5967
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5883..5902)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5937..5956
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6016..6032)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6016..6032)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6029..6051)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6040..6056
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6064..6070,6071..6088,6089..6094))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6109..6130
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6247..6264)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6418..7006)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6498..6517)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7177..7968,7969..8037))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7800..7819
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8038..8142)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8217..8236)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagc ttggtaccga gctcggatcc actagtaacg gccgccagtg
      721 tgctggaatt ctgcagatat ccatcacact ggcggccgct cgagcggact caacatgcgc
      781 tgctcgccgg gaggcgtctg gctggcgctg gccgcgtcgc tcctgcacgt gtccctgcaa
      841 ggcgagttcc agaggaagct ttacaaggag ctggtcaaga actacaatcc cctggagagg
      901 cccgtggcca atgactcgca accactcacc gtctacttct ccctgagcct cctgcagatc
      961 atggacgtgg atgagaagaa ccaagtttta accaccaaca tttggctgca aatgtcttgg
     1021 acagatcact atttacagtg gaatgtgtca gaatatccag gggtgaagac tgttcgtttc
     1081 ccagatggcc agatttggaa accagacatt cttctctata acagtgctga tgagcgcttt
     1141 gacgccacat tccacactaa cgtgttggtg aattcttctg ggcattgcca gtacctgcct
     1201 ccaggcatat tcaagagttc ctgctacatc gatgtacgct ggtttccctt tgatgtgcag
     1261 cactgcaaac tgaagtttgg gtcctggtct tacggaggct ggtccttgga tctgcagatg
     1321 caggaggcag atatcagtgg ctatatcccc aatggagaat gggacctagt gggaatcccc
     1381 ggcaagagga gtgaaaggtt ctatgagtgc tgcaaagagc cctaccctga tgtcaccttc
     1441 acagtgacca tgcgccgcag gacactctac tatggcctca acctgctgat cccctgtgtg
     1501 ctcatctccg ccctcgccct gctggtgttc ctgcttcctg cagattccgg ggagaagatt
     1561 tccctgggga taacagtctt actctctctt accgtcttca tgctgctcgt ggctgagatc
     1621 atgcccgcaa catccgattc ggtaccattg atagcccagt acttcgccag caccatgatc
     1681 atcgtgggcc tctcggtggt ggtgacggtg atcgtgctgc agtaccacca ccacgacccc
     1741 gacgggggca agatgcccaa gtggaccaga gtcatccttc tgaactggtg cgcgtggttc
     1801 ctgcgaatga agaggcccgg ggaggacaag gtgcgcccgg cctgccagca caagcagcgg
     1861 cgctgcagcc tggccagtgt ggagatgagc gccgtggcgc cgccgcccgc cagcaacggg
     1921 aacctgctgt acatcggctt ccgcggcctg gacggcgtgc actgtgtccc gacccccgac
     1981 tctggggtag tgtgtggccg catggcctgc gctggcgcca tggtgagcaa gggcgaggag
     2041 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     2101 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     2161 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttcggctac
     2221 ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     2281 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     2341 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     2401 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     2461 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     2521 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     2581 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccgcc
     2641 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     2701 gccgggatca ctctcggcat ggacgagctg tacaaggcag gcgcctcccc cacgcacgat
     2761 gagcacctcc tgcacggtgg gcaacccccc gagggggacc cggacttggc caagatcctg
     2821 gaggaggtcc gctacattgc caaccgcttc cgctgccagg acgaaagcga ggcggtctgc
     2881 agcgagtgga agttcgccgc ctgtgtggtg gaccgcctgt gcctcatggc cttctcggtc
     2941 ttcaccatca tctgcaccat cggcatcctg atgtcggctc ccaacttcgt ggaggccgtg
     3001 tccaaagact ttgcgtaacc acgcctggtt ctgtacatgt ggaaaactca cagatgggca
     3061 aggcctttgg cttggcgaga tttgggggtg ctaatccagg acagcattac acgccacaac
     3121 tccagtgttc ccttctggct gtcagtcgtg ttgcttacgg tttctttgtt actttaggta
     3181 gtagaatctc agcactttgt ttcatattct cagatgggct gatagatatc cttggcacat
     3241 ccgtaccatc ggtcagcagg gccactgagt agtcattttg cccattagcc cactgcctgg
     3301 aaagcccttc ggagagctcc ccatggctcc tcaccaccga gacagttggt tttgcatgtc
     3361 tgcatgaagg tctacctgaa aattcaacat ttgctttttg cttgtgtaca aacccagatt
     3421 gaagctaaaa taaaccagac tcactaaatc ctttccaaaa aaaaaaaaaa aaaaaaaaaa
     3481 ccggaattcc tgcagcccgg gggatccact agtccagtgt ggtggaattc tgcagatatc
     3541 cagcacagtg gcggccgctc gagtctagag ggcccgttta aacccgctga tcagcctcga
     3601 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     3661 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     3721 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     3781 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     3841 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     3901 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     3961 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     4021 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     4081 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     4141 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     4201 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     4261 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     4321 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     4381 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     4441 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     4501 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     4561 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     4621 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     4681 tcagcacgtg atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga
     4741 aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt
     4801 cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt
     4861 ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt
     4921 gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg
     4981 tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga
     5041 ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg
     5101 accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc
     5161 ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc
     5221 tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc
     5281 ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg
     5341 gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc
     5401 gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc
     5461 aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag
     5521 cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt
     5581 ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg
     5641 gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc
     5701 gagggcaaag gaatagcacg tgctacgaga tttcgattcc accgccgcct tctatgaaag
     5761 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     5821 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     5881 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     5941 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     6001 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     6061 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     6121 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     6181 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     6241 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     6301 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     6361 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     6421 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     6481 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     6541 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     6601 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     6661 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     6721 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     6781 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     6841 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     6901 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     6961 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     7021 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     7081 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     7141 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     7201 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     7261 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     7321 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     7381 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     7441 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     7501 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     7561 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     7621 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     7681 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     7741 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     7801 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     7861 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     7921 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     7981 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     8041 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     8101 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     8161 gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca
     8221 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc
     8281 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
     8341 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
     8401 acgcgtt
//
