LOCUS       sequence-347422-        8313 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for Hsf1 activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8313)
  AUTHORS   Boos F, Kramer L, Groh C, Jung F, Haberkant P, Stein F, Wollweber F,
            Gackstatter A, Zoller E, van der Laan M, Savitski MM, Benes V, 
            Herrmann JM
  TITLE     Mitochondrial protein-induced stress triggers a global adaptive 
            transcriptional programme.
  JOURNAL   Nat Cell Biol. 2019 Apr;21(4):442-451. doi: 
            10.1038/s41556-019-0294-5. Epub 2019 Mar 18.
  PUBMED    30886345
REFERENCE   2  (bases 1 to 8313)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-019-0294-5"; journalName: "Nat Cell Biol"; date: 
            "2019-04"; volume: "21"; issue: "4"; pages: "442-451"
COMMENT     Sequence Label: pNH605-HSE-YFP
FEATURES             Location/Qualifiers
     source          1..8313
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        442..849
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             2984..3700
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(3702..3718)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3702..3718)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5026..5045)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5027..5045)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5052..5069)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5052..5068)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5061..5083)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5209..5664
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5296..5315)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5506..5527
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6020..6039)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6139..6161
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6199..6217)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6302..6406
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6407..6475,6476..7267)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6625..6644)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7438..8026
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7927..7946
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8180..8197
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag ctcggaatta accctcacta aagggaacaa aagctgggcc ggccatttaa
      181 atgtttaaac ggccacgctg gccgaggaga acttctagta tatctacata cctaatatta
      241 ttgccttatt aaaaatggaa tcccaacgat tacatcaaaa tccacattct cttcaaaatc
      301 aattgtcctg tacttccttg ttcatgtgtg ttcaaaaacg ttatatttat aggataatta
      361 tactctattt ctcaacaagt aattggttgt ttggccgagc ggtctaaggc gcctgattca
      421 agaaatatct tgaccgcagt taactgtggg aatactcagg tatcgtaaga tgcaagagtt
      481 cgaatctctt agcaaccatt atttttttcc tcaacataac gagaacacac aggggcgcta
      541 tcgcacagaa tcaaattcga tgactggaaa ttttttgtta atttcagagg tcgcctgacg
      601 catatacctt tttcaactga aaaattggga gaaaaaggaa aggtgagagc gccggaaccg
      661 gcttttcata tagaatagag aagcgttcat gactaaatgc ttgcatcaca atacttgaag
      721 ttgacaatat tatttaagga cctattgttt tttccaatag gtggttagca atcgtcttac
      781 tttctaactt ttcttacctt ttacatttca gcaatatata tatatatatt tcaaggatat
      841 accattctaa tgtctgcccc taagttacca atagatagcg gtttaatata aggtttcaac
      901 aagaaatatt ttgtgaaaat caaaagttaa tagtttcact gtcgaaaata tgaaagttta
      961 agttttcagt tcatcccatt gtagttatca aatttctgtg tttcccgtat atgtgtgctc
     1021 tgtattcatc gctatcttga aactggttat gatcgttgcg catgtgacct gtatcgctca
     1081 aaagagcccg gcaccggtac cgggcaaaag cccctagcaa gctaaaatga ttcaactttc
     1141 gtagtaattt gttcaaccgg tcacagtaaa gtcaatgact aacttttgaa tcgaggatgt
     1201 atttccaatt gaaagcaatg tatatataaa ccattgtgtt gaagcttgtc ttacagtttt
     1261 cttgtatcct cccattgttc atatagtact taaagtacca taatccttaa agaacaaact
     1321 tgaaagagaa tacactatac acaactaata aataaagaat aatcatggct gtgaccaaga
     1381 caattgtagt tctaccaggt gaccatgttg gtcaagaaat cactgaagag gccattaaag
     1441 ttttaaatgc tattcaggaa tgtcgtccag acaaggtcaa tttcaagttt gatcatcatt
     1501 tgatcggtgg tgctgcaatt gatgccactg gtgttccatt accagacgaa gctttggagg
     1561 cctctaagaa agctgatgcc gtgcttctgg gtgctgttgg tggtccaaaa tggggtactg
     1621 gcgctgtcag accagaacaa ggtcttttga aaatccgtaa ggagttgcaa ttgtatgcta
     1681 atctaagacc atgtaatttt gcatctgatt ccttactaga tctatcgcca ttgaagcctg
     1741 aaattgcaag aggtacagat ttcgtcgttg ttagagaact agtgggtggt atttatttcg
     1801 gggagagaaa agaagatgaa ggtgatggtg tcgcctggga tagcgaaaag tattctgtgc
     1861 ccgaagttca aagaatcact agaatggctg ctttcatggc tctgcaacac aacccacctt
     1921 tacctatttg gtctctagat aaagccaacg ttctagcatc atcaagatta tggagaaaaa
     1981 cagtggaaga gacgattaag aatgaattcc cacaattgac tgttcaacat caattgattg
     2041 attccgctgc tatgatcttg gttaagaacc caactcatct aaatggtatt attatcacaa
     2101 acaatatgtt tggtgatatt atctccgatg aagcctccgt gatcccaggt tcgttaggat
     2161 tacttccatc tgcctccttg gcatctcttc cagataagaa tacggcattt ggtttatatg
     2221 agccatgtca tggctctgct ccagacttac caaagggaaa ggttaacccc gtagcaacaa
     2281 tcctatctgc tgctatgatg ttgaagttgt ctctggacct gttcgaagaa ggtgaaatta
     2341 tagaacaagc agtcaagaaa gtgttggatt ctggtatcag aacagcggat ctaaagggta
     2401 ccaactctac tactgaggtc ggtgatgcag tagcgaaagc tgtcagggaa ctattagctt
     2461 agacttagta gatgtacagt tgcatacata agtctgaaca gaaagaagta aatatcatat
     2521 ctagttcggt ttagaaatca gtgactatat tggttttgac cagatcgcaa tccttgcaag
     2581 catttagtaa tgactatcaa accggtgaat catctgataa ggctatatta gcttgtgcat
     2641 tcgcatgtat cgggaaacga acggtaccgg gcccctagaa gcttctagaa gcttctagaa
     2701 gcttctagaa gcttctagag gatccccgtc gagcagatcc gccaggcgtg tatatatagc
     2761 gtggatggcc aggcaacttt agtgctgaca catacaggca tatatatatg tgtgcgacga
     2821 cacatgatca tatggcatgc atgtgctctg tatgtatata aaactcttgt tttcttcttt
     2881 tctctaaata ttctttcctt atacattagg acctttgcag cataaattac tatacttcta
     2941 tagacacaca aacacaaata cacacactaa attaatactc gagatgtcta aaggtgaaga
     3001 attattcact ggtgttgtcc caattttggt tgaattagat ggtgatgtta atggtcacaa
     3061 attttctgtc tccggtgaag gtgaaggtga tgctacttac ggtaaattga ccttaaaatt
     3121 gatttgtact actggtaaat tgccagttcc atggccaacc ttagtcacta ctttaggtta
     3181 tggtttgcaa tgttttgcta gatacccaga tcatatgaaa caacatgact ttttcaagtc
     3241 tgccatgcca gaaggttatg ttcaagaaag aactattttt ttcaaagatg acggtaacta
     3301 caagaccaga gctgaagtca agtttgaagg tgatacctta gttaatagaa tcgaattaaa
     3361 aggtattgat tttaaagaag atggtaacat tttaggtcac aaattggaat acaactataa
     3421 ctctcacaat gtttacatca ctgctgacaa acaaaagaat ggtatcaaag ctaacttcaa
     3481 aattagacac aacattgaag atggtggtgt tcaattagct gaccattatc aacaaaatac
     3541 tccaattggt gatggtccag tcttgttacc agacaaccat tacttatcct atcaatctgc
     3601 cttatccaaa gatccaaacg aaaagagaga ccacatggtc ttgttagaat ttgttactgc
     3661 tgctggtatt acccatggta tggatgaatt gtacaaataa ggatccacta gttctagaca
     3721 cctgcaacat gcgtgagtaa ataagcggcc gccaccgcgg tggagctcta agcaaatagc
     3781 taaattatat acgaattaat attatgatta agtgtttacg tgagtgcgat atttttatta
     3841 ctatcttata cagttgtata tactctataa aatgagttgt ctattaatta acgcgatgaa
     3901 tgctttctgg gtttacctct ccaacaactc tagtttactt ctcaatacat tcaattgtat
     3961 ttgatttgtc aatacttcat cattaatcaa ttctatagtt ttgtttttct cgtttatttc
     4021 caaatttaat gcatcaattt tattattcaa tttgtcgttg attttggtta atgattttat
     4081 ggtttgatct ctggcattga ttgtttgtgt tagtttttca ttattgataa ttaaattatt
     4141 taagttagtt atcaactcgg tgttttcaag tttcaagttt tcaatttctt tagagtttat
     4201 tagatttgtc aaagtttctg aattgcttga ttggtcctgt agaagagtat ttgttgttgt
     4261 ggataattga ttcaattttt gagacaattg ctggaaggcg ttgaaatatc tagcatcaat
     4321 ctcatggttt ttttcccgag agtctcgtag attcaattgt tttaatatat cttgggacca
     4381 ctcttgattt gaactcatgg aaattaaact gggtgttgtg ttgtggtgta atgattgtac
     4441 cccctttgct tataattgtg tggccaccga agtcggtgat gctgtcgccg aagaagttaa
     4501 gaaaatcctt gcttaaaaag attctctttt tttatgatat ttgtacataa actttataaa
     4561 tgaaattcat aatagaaacg acacgaaatt acaaaatgga atatgttcat agggtagacg
     4621 aaactatata cgcaatctac atacatttat caagaaggag aaaaaggagg atgtaaagga
     4681 atacaggtaa gcaaattgat actaatggct caacgtgata aggaaaaaga attgcacttt
     4741 aacattaata ttgacaagga ggagggcacc acacaaaaag ttaggtgtaa cagaaaatca
     4801 tgaaactatg attcctaatt tatatattgg aggattttct ctaaaaaaaa aaaaatacaa
     4861 caaataaaaa acactcaatg acctgaccat ttgatggagt ttaagtcaat acctccttga
     4921 accatttccc ataatggtga aagttccctc aagaatttta ctctgtcaga aacggcctta
     4981 acgacgtagg ccggccattt aaatgtttaa acggccacgc tggcccccta tagtgagtcg
     5041 tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     5101 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     5161 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggac gcgccctgta
     5221 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     5281 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     5341 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     5401 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     5461 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     5521 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     5581 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     5641 acaaaatatt aacgcttaca atttcctgat gcggtatttt ctccttacgc atctgtgcgg
     5701 tatttcacac cgcatagggt aataactgat ataattaaat tgaagctcta atttgtgagt
     5761 ttagtattag gatctctacc cttggcgaaa agtcctctgc caacaatgat gatatctgat
     5821 ccaccactta caacttcgtc gacggttctg tactgctgac ccaatatgcg tatatatacc
     5881 aatctaagtc tgtgctcctt ccttcgttct tccttctgtt cggagattac cgaatcaaaa
     5941 aaatttcaag gaaaccgaaa tcaaaaaaaa gaataaaaaa aaaatgatga attgaaaagg
     6001 tggtatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     6061 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     6121 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     6181 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     6241 taatggtttc ttagacgtcg gcgcgccgag gacgtcaggt ggcacttttc ggggaaatgt
     6301 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     6361 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     6421 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     6481 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     6541 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     6601 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     6661 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     6721 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     6781 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     6841 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     6901 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     6961 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     7021 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     7081 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     7141 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     7201 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     7261 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     7321 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     7381 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     7441 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     7501 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     7561 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     7621 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     7681 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     7741 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     7801 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     7861 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     7921 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     7981 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     8041 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     8101 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     8161 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     8221 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     8281 cgactggaaa gcgggcagtg agcgcaacgc aat
//
