LOCUS       sequence-347452-        5169 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  13S-S Candidatus "Scalindua brodae" Twin- Strep SUMO tagged RAMP 
            (codon-optimization).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5169)
  AUTHORS   van Beljouw SPB, Haagsma AC, Rodriguez-Molina A, van den Berg DF, 
            Vink JNA, Brouns SJJ
  TITLE     The gRAMP CRISPR-Cas effector is an RNA endonuclease complexed with 
            a caspase-like peptidase.
  JOURNAL   Science. 2021 Sep 17;373(6561):1349-1353. doi: 
            10.1126/science.abk2718. Epub 2021 Aug 26.
  PUBMED    34446442
REFERENCE   2  (bases 1 to 5169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abk2718"; journalName: "Science"; date: 
            "2021-09-17- 17"; volume: "373"; issue: "6561"; pages: "1349-1353"
COMMENT     Sequence Label: pTU398
FEATURES             Location/Qualifiers
     source          1..5169
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgaaaagca acgacatgaa cattaccgtg gaactgacct tttttgaacc gtatcgtctg
       61 gttgaatggt ttgattggga tgcacgtaaa aaaagccata gcgcaatgcg tggtcaggca
      121 tttgcacagt ggacctggaa aggtaaaggt cgtaccgcag gtaaaagctt tattaccggt
      181 acactggttc gtagcgcagt tattaaagca gttgaagaac tgctgagcct gaataatggt
      241 aaatgggaag gtgttccgtg ttgcaatggt agctttcaga ccgatgaaag caaaggtaaa
      301 aaaccgagct ttctgcgtaa acgtcatacc ctgcagtggc aggcaaataa caaaaacatt
      361 tgcgataaag aagaggcctg tccgttttgt attctgctgg gtcgttttga taatgccggt
      421 aaagtgcatg aacgcaacaa agattatgat atccacttca gcaacttcga cctggatcac
      481 aaacaagaaa aaaatgatct gcgcctggtt gatattgcaa gcggtcgtat tctgaatcgt
      541 gttgattttg ataccggcaa agccaaagat tactttcgta cctgggaagc agattatgaa
      601 acctatggca cctataccgg tcgcattacc ctgcgtaatg aacatgcaaa aaaactgctg
      661 ctggcaagcc tgggttttgt tgataaactg tgtggtgcac tgtgtcgtat tgaggttatc
      721 aaaaaaagcg aaagtccgct gccgagcgat accaaagaac agagctatac aaaagatgat
      781 accgttgaag ttctgagcga agatcataat gatgaactgc gcaaacaggc cgaagttatt
      841 gttgaagcat ttaagcagaa cgataaactg gaaaaaattc gcattctggc agatgcaatt
      901 cgtaccctgc gcctgcatgg tgaaggtgtg attgaaaaag atgagctgcc ggatggtaaa
      961 gaagaacgcg ataaaggtca tcatctgtgg gatattaaag ttcagggcac cgcactgcgt
     1021 accaaactga aagaactgtg gcagagcaat aaagatattg gctggcgcaa atttaccgaa
     1081 atgctgggta gcaatctgta cctgatctat aagaaagaaa ccggtggtgt tagcacccgt
     1141 tttcgcatcc tgggtgatac cgagtattat agcaaagcac atgatagcga aggtagcgac
     1201 ctgtttattc cggttacacc gcctgaaggt attgaaacca aagaatggat tattgtgggt
     1261 cgcctgaaag cagcaacccc gttttatttc ggtgttcagc agccgagtga tagcattccg
     1321 ggtaaagaga aaaaatcaga agatagcctg gtcatcaatg aacacaccag ctttaacatc
     1381 ctgctggata aagaaaatcg ttatcgtatt ccgcgtagtg cactgcgtgg tgccctgcgt
     1441 cgcgatctgc gtaccgcatt tggtagcggt tgtaatgtta gcttaggtgg tcagattctg
     1501 tgcaattgta aagtgtgtat tgaaatgcgt cgcatcacac tgaaagatag cgttagcgat
     1561 ttttcagaac ctccggaaat tcgctatcgc attgcaaaaa atccgggtac agcaaccgtg
     1621 gaagatggta gtctgtttga tattgaagtt ggtccggaag gcctgacctt tccgtttgtt
     1681 ctgcgttatc gtggtcataa atttccagaa cagctgagca gcgttattcg ttattgggaa
     1741 gaaaatgatg gcaaaaatgg tatggcatgg ttaggtggcc tggatagcac cggtaaaggc
     1801 cgttttgccc tgaaagacat taaaatcttt gagtgggatc tgaaccagaa aatcaacgaa
     1861 tatatcaaag aacgcggtat gcgtggcaaa gaaaaagaat tactggaaat gggtgaaagc
     1921 agtctgcctg atggtctgat tccgtataaa ttctttgaag aacgtgaatg cctgtttccg
     1981 tacaaagaaa acctgaaacc gcagtggtca gaagttcagt ataccattga agtgggttca
     2041 ccgctgctga ccgcagatac cattagcgca ctgaccgaac cgggtaatcg tgatgcaatt
     2101 gcctacaaaa aacgcgtgta taacgatggc aataatgcca ttgaaccgga accgcgtttt
     2161 gcagttaaaa gtgaaaccca tcgtggtatt tttcgcaccg cagttggtcg tcgtaccggt
     2221 gatctgggca aagaagatca cgaagattgt acctgtgata tgtgcattat ctttggcaat
     2281 gagcatgaga gcagcaaaat tcgttttgaa gatctggaac tgatcaacgg caacgaattt
     2341 gaaaagctgg aaaaacatat tgaccacgtg gccattgatc gttttacagg tggcgcactg
     2401 gacaaagcaa aatttgatac ctatccgctg gcaggtagcc cgaaaaaacc gctgaaactg
     2461 aagggtcgct tttggattaa aaagggtttt agcggtgatc acaagctgct gattaccaca
     2521 gcactgagcg atattcgtga tggcctgtat cctctgggta gtaaaggtgg tgttggttat
     2581 ggttgggttg caggtattag cattgatgat aatgtgccgg atgactttaa agagatgatc
     2641 aacaagacag aaatgccgct gccggaagaa gtggaagaaa gcaataatgg tccgatcaat
     2701 aacgattatg ttcatccggg tcatcagagc ccgaaacagg atcataaaaa caagaacatc
     2761 tattatccgc attattttct ggacagcggc agcaaagtgt atcgcgaaaa agatattatc
     2821 acccacgaag aattcaccga ggaactgctg tcaggcaaaa ttaactgtaa acttgaaacc
     2881 ctgacaccgc tgattattcc ggataccagt gatgaaaatg gtctgaaact tcagggtaat
     2941 aaaccgggtc ataagaacta caaattcttc aacattaatg gcgaactgat gattccgggt
     3001 tcagaactgc gtggcatgct gcgcacccat tttgaagcac tgaccaaaag ctgttttgcc
     3061 atttttggtg aagatagcac cctgagctgg cgtatgaatg cagatgagaa agattacaaa
     3121 atcgatagca acagcatccg caaaatggaa agccagcgta atccgaaata tcgcattccg
     3181 gacgaactgc agaaagagct gcgtaatagc ggtaatggtc tgtttaatcg tctgtatacc
     3241 agcgaacgtc gtttttggag tgatgtgagt aacaaatttg agaacagcat cgattacaaa
     3301 cgcgaaattc tgcgttgtgc aggtcgtccg aaaaactata aaggcggtat tattcgtcag
     3361 cgtaaagata gtctgatggc cgaagaactg aaagttcatc gtctgcctct gtatgataac
     3421 tttgatattc ctgatagcgc ctacaaagcc aacgatcatt gtcgtaaaag cgcaacctgt
     3481 agcaccagcc gtggttgtcg tgaacgtttt acctgtggca ttaaagtgcg tgataaaaat
     3541 cgcgtttttc tgaatgcagc caataataat cgccagtacc tgaacaacat caaaaagtcc
     3601 aatcacgatc tgtatctgca gtatctgaaa ggcgaaaaaa agatccgctt caacagcaaa
     3661 gttattacag gtagcgaacg tagcccgatt gatgttattg cagaactgaa tgaacgtggt
     3721 cgtcagaccg gttttatcaa actgagcggt ctgaataaca gcaataaaag ccagggcaat
     3781 accggcacca catttaatag tggttgggat cgctttgaac tgaatatact gctggatgat
     3841 ctggaaaccc gtccgagcaa aagcgattat ccgcgtccgc gtctgctgtt taccaaagat
     3901 cagtatgaat acaacatcac caaacgttgc gaacgcgtgt ttgaaattga taaaggcaac
     3961 aaaacaggct atccggtgga tgatcagatc aaaaagaact acgaagatat cctggacagc
     4021 tatgatggca tcaaagatca agaagttgcc gaacgctttg atacatttac ccgtggtagc
     4081 aagctgaaag ttggcgatct ggtttatttt catatcgacg gcgataacaa aattgacagc
     4141 ctgattccgg ttcgtattag ccgtaaatgt gcaagcaaaa ccttaggtgg caaattagat
     4201 aaagcactgc atccgtgcac cggtctgtca gatggtctgt gtccgggttg tcacctgttt
     4261 ggcaccaccg attataaagg tcgcgttaaa tttggcttcg ccaaatatga aaacggtcct
     4321 gaatggctga ttacgcgtgg taataatccg gaacgtagtc tgaccctggg tgtgctggaa
     4381 tcaccgcgtc cggcattttc aattccggat gatgaaagtg aaattccggg tcgtaaattc
     4441 tatctgcatc acaatggttg gcgcattatt cgccagaaac aactggaaat tcgtgaaacc
     4501 gttcagccgg aacgcaatgt taccaccgaa gtgatggata aaggtaacgt gtttagcttt
     4561 gatgtgcgct ttgaaaatct gcgtgaatgg gaactgggtc tgctgctgca gagtctggat
     4621 cctggtaaaa acattgcaca taaacttggt aaaggcaaac cgtatggttt tggcagcgtg
     4681 aaaatcaaga ttgatagcct gcataccttc aagattaaca gcaacaacga caaaatcaaa
     4741 cgtgttccgc agagtgatat ccgcgagtat attaacaaag gctaccagaa actgattgaa
     4801 tggtcaggta ataatagcat ccagaaaggt aatgtgctgc cgcagtggca tgttattccg
     4861 catattgaca aactgtacaa actgctgtgg gttccgtttc tgaacgatag caaactggaa
     4921 ccggatgttc gttatccggt tctgaatgaa gaatccaaag gttatattga gggcagcgat
     4981 tacacctata aaaagctggg agataaagat aacctgccgt ataaaacccg tgttaaaggt
     5041 ctgaccacac cgtggtcacc gtggaatccg tttcaggtga ttgccgaaca tgaagaacaa
     5101 gaagtgaacg ttaccggtag ccgtccgagt gttaccgata aaattgaacg tgatggtaaa
     5161 atggtgtaa
//
