LOCUS       sequence-347459-         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  13S-S Candidatus "Scalindua brodae" Twin- Strep SUMO tagged RAMP 
            (codon-optimization) enriched with CRISPR array spacer 1 (five 
            times).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   van Beljouw SPB, Haagsma AC, Rodriguez-Molina A, van den Berg DF, 
            Vink JNA, Brouns SJJ
  TITLE     The gRAMP CRISPR-Cas effector is an RNA endonuclease complexed with 
            a caspase-like peptidase.
  JOURNAL   Science. 2021 Sep 17;373(6561):1349-1353. doi: 
            10.1126/science.abk2718. Epub 2021 Aug 26.
  PUBMED    34446442
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abk2718"; journalName: "Science"; date: 
            "2021-09-17- 17"; volume: "373"; issue: "6561"; pages: "1349-1353"
COMMENT     Sequence Label: pTU422
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     34..53
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        34..52
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    53..77
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             634..678
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(716..734)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      730..777
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata ggggaattgt
       61 gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga gatatacata
      121 tgtacaaaat ggccccttct cgccatatac gtaacctcag agttgttgga gggttatgaa
      181 acaagagaag gacttaatgt cacggtaccc aattttctgc cccggactcc acggctgtta
      241 ctagaggtta tgaaacaaga gaaggactta atgtcacggt acccaatttt ctgccccgga
      301 ctccacggct gttactagag gttatgaaac aagagaagga cttaatgtca cggtacccaa
      361 ttttctgccc cggactccac ggctgttact agaggttatg aaacaagaga aggacttaat
      421 gtcacggtac ccaattttct gccccggact ccacggctgt tactagaggt tatgaaacaa
      481 gagaaggact taatgtcacg gtacccaatt ttctgccccg gactccacgg ctgttactag
      541 aggttatgaa acaagagaag gacttaatgt cacggtacca attggatatc ggccggccac
      601 gcgatcgctg acgtcggtac cctcgagtct ggtaaagaaa ccgctgctgc gaaatttgaa
      661 cgccagcaca tggactcgtc tactagcgca gcttaattaa cctaggctgc tgccaccgct
      721 gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg
      781 aaacctc
//
