LOCUS       sequence-347568-       10296 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible overexpression of SRF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10296)
  AUTHORS   Romero-Tejeda M, Fonoudi H, Weddle CJ, DeKeyser JM, Lenny B, 
            Fetterman KA, Magdy T, Sapkota Y, Epting C, Burridge PW
  TITLE     A novel transcription factor combination for direct reprogramming to
            a spontaneously contracting human cardiomyocyte-like state.
  JOURNAL   J Mol Cell Cardiol. 2023 Jul 6:S0022-2828(23)00106-2. doi: 
            10.1016/j.yjmcc.2023.06.005.
  PUBMED    37421991
REFERENCE   2  (bases 1 to 10296)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Cell 
            Cardiol. 2023 Jul 6:S0022-2828(23)00106-2. doi: 
            10.1016/j.yjmcc.2023.06.005."
COMMENT     Sequence Label: tetO-SRF
FEATURES             Location/Qualifiers
     source          1..10296
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(49..68)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        240..619
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        621..819
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             837..1017
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1064..1189
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1682..1915
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2100..2144
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2442..2559
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2612..2630,2631..2653,2654..2672,2673..2695,2696..2714,
                     2715..2737,2738..2756,2757..2779,2780..2798,2799..2821,
                     2822..2840,2841..2863,2864..2882)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2915..2953
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2950..2974
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     3053..3072
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3053..3071
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3103..3127
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4697..4720
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4733..4777
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     protein_bind    complement(4822..4846)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4872..4913
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4923..4940
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    4990..5578
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5043..5063)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5461..5472)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5581..5597)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5582..5598)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6103..6283
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6309..6326)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6315..6539
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6585..7013
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6672..6691)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6882..6903
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7022..7042)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7027..7356
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7207..7342
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7269..7288
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7404..7451
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7470..7844
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7974..8095
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8011..8030)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8065..8084
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8144..8160)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8144..8160)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8157..8179)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8168..8184
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8192..8198,8199..8216,8217..8222))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8237..8258
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8375..8392)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8546..9134)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8626..8645)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9305..10096,10097..10165))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9928..9947
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10166..10270)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc
       61 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
      121 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
      181 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg
      241 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
      301 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      361 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      421 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      481 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      541 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      601 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      661 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      721 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      781 gggcggtagg cgtgtacggt gggaggtcta tataagcagc gcgttttgcc tgtactgggt
      841 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
      901 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
      961 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     1021 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     1081 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     1141 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     1201 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     1261 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     1321 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     1381 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     1441 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     1501 caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata
     1561 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
     1621 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
     1681 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
     1741 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
     1801 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
     1861 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
     1921 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
     1981 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
     2041 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
     2101 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
     2161 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
     2221 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
     2281 tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag
     2341 aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga tcggcactgc
     2401 gtgcgccaat tctgcagaca aatggcagta ttcatccaca attttaaaag aaaagggggg
     2461 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
     2521 aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc
     2581 agagatccag tttggttatc gagtttacca ctccctatca gtgatagaga aaagtgaaag
     2641 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct
     2701 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
     2761 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
     2821 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
     2881 gaaaagtgaa agtcgagctc ggtacccggg tcgaggtagg cgtgtacggt gggaggccta
     2941 tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt
     3001 tgacctccat agaagacacc gggaccgatc cagcctccgc ggccccgaat tgtaatacga
     3061 ctcactatag ggagacccaa gctggctagg taagcttgat caacaagttt gtacaaaaaa
     3121 gcaggctctt taaaggaacc aattcagtcg acgccgccgc gatcgcatgt taccgaccca
     3181 agctggggcc gcggcggctc tgggccgggg ctcggccctg gggggcagcc tgaaccggac
     3241 cccgacgggg cggccgggcg gcggcggcgg gacacgcggg gctaacgggg gccgggtccc
     3301 cgggaatggc gcggggctcg ggcccggccg cctggagcgg gaggctgcgg cagcggcggc
     3361 aaccaccccg gcgcccaccg cgggggccct ctacagcggc agcgagggcg actcggagtc
     3421 gggcgaggag gaggagctgg gcgccgagcg gcgcggcctg aagcggagcc tgagcgagat
     3481 ggagatcggt atggtggtcg gtgggcccga ggcgtcggca gcggccaccg ggggctacgg
     3541 gccggtgagc ggcgcggtga gcggggccaa gccgggtaag aagacccggg gccgcgtgaa
     3601 gatcaagatg gagttcatcg acaacaagct gcggcgctac acgaccttca gcaagaggaa
     3661 gacgggcatc atgaagaagg cctatgagct gtccacgctg acagggacac aggtgctgtt
     3721 gctggtggcc agtgagacag gccatgtgta tacctttgcc acccgaaaac tgcagcccat
     3781 gatcaccagt gagaccggca aggcactgat tcagacctgc ctcaactcgc cagactctcc
     3841 accccgttca gaccccacaa cagaccagag aatgagtgcc actggctttg aagagacaga
     3901 tctcacctac caggtgtcgg agtctgacag cagtggggag accaaggaca cactgaagcc
     3961 ggcgttcaca gtcaccaacc tgccgggtac aacctccacc atccaaacag cacctagcac
     4021 ctctaccacc atgcaagtca gcagcggccc ctcctttccc atcaccaact acctggcacc
     4081 agtgtctgct agtgtcagcc ccagtgctgt cagcagtgcc aatgggactg tgctgaagag
     4141 tacaggcagc ggccctgtct cctctggggg ccttatgcag ctgcctacca gcttcaccct
     4201 catgcctggt ggggcagtgg cccagcaggt cccagtgcag gccattcaag tgcaccaggc
     4261 cccacagcaa gcgtctccct cccgtgacag cagcacagac ctcacgcaga cctcctccag
     4321 cgggacagtg acgctgcccg ccaccatcat gacgtcatcc gtgcccacaa ctgtgggtgg
     4381 ccacatgatg taccctagcc cgcatgcggt gatgtatgcc cccacctcgg gcctgggtga
     4441 tggcagcctc accgtgctga atgccttctc ccaggcacca tccaccatgc aggtgtcaca
     4501 cagccaggtc caggagccag gtggcgtccc ccaggtgttc ctgacagcat catctgggac
     4561 agtgcagatc cctgtttcag cagttcagct ccaccagatg gctgtgatag ggcagcaggc
     4621 cgggagcagc agcaacctca ccgagctaca ggtggtgaac ctggacaccg cccacagcac
     4681 caagagtgaa acgcgtgact acaaagacga tgacgataaa ggtgatgggt ccggcctgaa
     4741 cgacatcttc gaggctcaga aaatcgaatg gcacgaaggc gcgccgagct cgaggtaggc
     4801 ggccgcactc gagatatcta gacccagctt tcttgtacaa agtggttgat ctagagggcc
     4861 cgcggttcga aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg
     4921 gtcatcatca ccatcaccat tgagtttaaa cccgctgatc agcctcaatt cgatatcaag
     4981 cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     5041 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     5101 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     5161 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     5221 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     5281 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     5341 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     5401 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     5461 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     5521 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat
     5581 cgataccgtc gacctcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca
     5641 gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca
     5701 gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     5761 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatatc
     5821 cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa ctacacacca
     5881 gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
     5941 caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca ccctgtgagc
     6001 ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga cagccgccta
     6061 gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct ctggttagac
     6121 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     6181 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     6241 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg
     6301 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     6361 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     6421 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     6481 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     6541 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     6601 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     6661 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     6721 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     6781 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     6841 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     6901 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     6961 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
     7021 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     7081 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     7141 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     7201 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     7261 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
     7321 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
     7381 attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag
     7441 tataatacga caaggtgagg aactaaacca tggccaagtt gaccagtgcc gttccggtgc
     7501 tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc gggttctccc
     7561 gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc ctgttcatca
     7621 gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc
     7681 tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg gacgcctccg
     7741 ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc
     7801 cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg ctacgagatt
     7861 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     7921 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     7981 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     8041 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     8101 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     8161 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     8221 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     8281 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     8341 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     8401 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     8461 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     8521 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     8581 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     8641 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     8701 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     8761 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     8821 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     8881 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     8941 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     9001 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     9061 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     9121 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     9181 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     9241 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     9301 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     9361 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     9421 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     9481 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     9541 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     9601 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     9661 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     9721 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     9781 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     9841 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     9901 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     9961 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    10021 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    10081 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    10141 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    10201 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    10261 gggttccgcg cacatttccc cgaaaagtgc cacctg
//
