LOCUS       sequence-347569-        9951 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible overexpression of TBX20.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9951)
  AUTHORS   Romero-Tejeda M, Fonoudi H, Weddle CJ, DeKeyser JM, Lenny B, 
            Fetterman KA, Magdy T, Sapkota Y, Epting C, Burridge PW
  TITLE     A novel transcription factor combination for direct reprogramming to
            a spontaneously contracting human cardiomyocyte-like state.
  JOURNAL   J Mol Cell Cardiol. 2023 Jul 6:S0022-2828(23)00106-2. doi: 
            10.1016/j.yjmcc.2023.06.005.
  PUBMED    37421991
REFERENCE   2  (bases 1 to 9951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Cell 
            Cardiol. 2023 Jul 6:S0022-2828(23)00106-2. doi: 
            10.1016/j.yjmcc.2023.06.005."
COMMENT     Sequence Label: tetO-TBX20
FEATURES             Location/Qualifiers
     source          1..9951
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(49..68)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        240..619
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        621..819
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             837..1017
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1064..1189
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1682..1915
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2100..2144
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2442..2559
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2612..2630,2631..2653,2654..2672,2673..2695,2696..2714,
                     2715..2737,2738..2756,2757..2779,2780..2798,2799..2821,
                     2822..2840,2841..2863,2864..2882)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2915..2953
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2950..2974
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     3053..3072
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3053..3071
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3103..3127
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4477..4501)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4527..4568
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4578..4595
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    4645..5233
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4698..4718)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5116..5127)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5236..5252)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5237..5253)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5758..5938
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5964..5981)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5970..6194
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6240..6668
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6327..6346)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6537..6558
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6677..6697)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6682..7011
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6862..6997
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6924..6943
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7059..7106
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7125..7499
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7629..7750
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7666..7685)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7720..7739
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7799..7815)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7799..7815)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7812..7834)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7823..7839
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7847..7853,7854..7871,7872..7877))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7892..7913
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8030..8047)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8201..8789)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8281..8300)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8960..9751,9752..9820))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9583..9602
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9821..9925)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc
       61 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
      121 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
      181 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg
      241 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
      301 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      361 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      421 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      481 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      541 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      601 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      661 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      721 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      781 gggcggtagg cgtgtacggt gggaggtcta tataagcagc gcgttttgcc tgtactgggt
      841 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
      901 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
      961 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     1021 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     1081 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     1141 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     1201 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     1261 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     1321 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     1381 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     1441 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     1501 caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata
     1561 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
     1621 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
     1681 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
     1741 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
     1801 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
     1861 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
     1921 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
     1981 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
     2041 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
     2101 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
     2161 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
     2221 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
     2281 tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag
     2341 aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga tcggcactgc
     2401 gtgcgccaat tctgcagaca aatggcagta ttcatccaca attttaaaag aaaagggggg
     2461 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
     2521 aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc
     2581 agagatccag tttggttatc gagtttacca ctccctatca gtgatagaga aaagtgaaag
     2641 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct
     2701 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
     2761 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
     2821 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
     2881 gaaaagtgaa agtcgagctc ggtacccggg tcgaggtagg cgtgtacggt gggaggccta
     2941 tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt
     3001 tgacctccat agaagacacc gggaccgatc cagcctccgc ggccccgaat tgtaatacga
     3061 ctcactatag ggagacccaa gctggctagg taagcttgat caacaagttt gtacaaaaaa
     3121 gcaggcttca ccatggagtt cacggcgtcc cccaagcccc aactctcctc tcgggccaac
     3181 gccttctcca ttgccgcgct catgtcgagc ggcggctcta aggagaagga ggcgacggag
     3241 aacacaatca aacccctgga gcaatttgtg gagaagtcgt cctgtgccca gcccctgggt
     3301 gagctgacca gcctggatgc tcatggggag tttggtggag gcagtggcag cagcccgtcc
     3361 tcctcctctc tgtgcactga gccactgatc cccaccaccc ccatcatccc cagtgaggaa
     3421 atggccaaaa ttgcctgcag cctggagacc aaggagcttt gggacaaatt ccatgagctg
     3481 ggcaccgaga tgatcatcac caagtcgggc aggaggatgt ttccaaccat ccgggtgtcc
     3541 ttttcggggg tggatcctga ggccaagtac atagtcctga tggacatcgt ccctgtggac
     3601 aacaagaggt accgctacgc ctaccaccgg tcctcctggc tggtggctgg caaggccgac
     3661 ccgccgttgc cagccaggct ctatgtgcat ccagattctc cttttaccgg tgagcaacta
     3721 ctcaaacaga tggtgtcttt tgaaaaggtg aaactcacca acaatgaact ggatcaacat
     3781 ggccatataa ttttgaactc aatgcataag taccagccaa gggtgcacat cattaagaag
     3841 aaagaccaca cagcctcatt gctcaacctg aagtctgaag aatttagaac tttcatcttt
     3901 ccagaaacag tttttacggc agtcactgcc taccagaatc aactgataac gaagctgaaa
     3961 atagatagca atccttttgc caaaggattc cgggattcct ccaggctcac tgacattgag
     4021 agggaaagtg tggagagcct gattcaaaag cattcctatg cacgctcacc catccgtacc
     4081 tacggaggag aagaagatgt cttgggggat gagagtcaga caaccccaaa tcgagggtca
     4141 gcctttacaa catctgataa tttgtctctc agctcctggg tatcatcttc ttccagtttt
     4201 cctgggtttc agcacccaca gtccctgact gctcttggca ccagcacagc atccatagca
     4261 acacccattc ctcaccccat ccagggttct ctgccaccat atagccgact gggaatgcct
     4321 ctgacaccat cggccattgc cagctccatg caagggagtg gccccacatt cccttcattc
     4381 cacatgccgc gataccatca ctattttcag caggggccct atgctgccat tcaaggacta
     4441 cgccattcct ctgctgtgat gacgccattt gtatgaaccc agctttcttg tacaaagtgg
     4501 ttgatctaga gggcccgcgg ttcgaaggta agcctatccc taaccctctc ctcggtctcg
     4561 attctacgcg taccggtcat catcaccatc accattgagt ttaaacccgc tgatcagcct
     4621 caattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
     4681 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     4741 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     4801 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     4861 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     4921 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     4981 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     5041 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     5101 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     5161 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     5221 gccgcctccc cgcatcgata ccgtcgacct cgagacctag aaaaacatgg agcaatcaca
     5281 agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag
     5341 gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct
     5401 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     5461 cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg
     5521 cagaactaca caccagggcc agggatcaga tatccactga cctttggatg gtgctacaag
     5581 ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg
     5641 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg
     5701 tttgacagcc gcctagcatt tcatcacatg gcccgagagc tgcatccgga ctgtactggg
     5761 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     5821 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     5881 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg
     5941 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     6001 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     6061 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     6121 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     6181 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     6241 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     6301 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     6361 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     6421 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     6481 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     6541 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     6601 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     6661 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     6721 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     6781 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     6841 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     6901 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     6961 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     7021 ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat
     7081 agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca
     7141 gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc
     7201 ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg
     7261 tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg
     7321 tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact
     7381 tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt
     7441 tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac
     7501 acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     7561 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     7621 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     7681 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     7741 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     7801 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     7861 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     7921 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     7981 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     8041 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     8101 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     8161 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     8221 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     8281 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     8341 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     8401 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     8461 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     8521 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     8581 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     8641 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     8701 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     8761 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     8821 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     8881 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     8941 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     9001 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     9061 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     9121 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     9181 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     9241 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     9301 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     9361 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     9421 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     9481 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     9541 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     9601 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     9661 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     9721 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     9781 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     9841 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     9901 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct g
//
