LOCUS       sequence-347594-        9428 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Destination vector for use 
            with pMuLE Entry vectors. Co-expresses IFP1.4 under the control of a
            PGK promoter. Compatible with MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9428)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 9428)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE Lenti Dest IFP1.4
FEATURES             Location/Qualifiers
     source          1..9428
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1575..1691
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1748..1872
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1897..1902,1903..1920,1921..1927)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1981..2640
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2047..2066)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2982..3287
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3166..3185
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3328..3452)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    3483..4071
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3536..3556)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3954..3965)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4085..4584
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4120..4143)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4517..4536
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      4626..4635
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4632..5618
                     /codon_start=1
                     /product="bacteriophytochrome-based monomeric infrared 
                     fluorescent protein (Shu et al., 2009)"
                     /label=IFP1.4
                     /translation="MARDPLPFFPPLYLGGPEITTENCEREPIHIPGSIQPHGALLTAD
                     GHSGEVLQVSLNAATFLGQEPTVLRGQTLAALLPEQWPALQAALPPGCPDALQYRATLD
                     WPAAGHLSLTVHRVAELLILEFEPTEAWDSIGPHALRNAMFALESAPNLRALAEVATQT
                     VRELTGFDRVMLYKFAPDATGEMIAEARREGMQAFLGHRFPASHTPAQARALYTRHLLR
                     LTADTRAAAVPLDPVLNPQTNAPTPLGGAVLRATSPMHMQYLRNMGVGSSLSVSVVVGG
                     QLWGLIVCHHQTPYVLPPDLRTTLEELGRKLSGQVQRKEAGMDELYK"
     LTR             5720..5953
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6025..6146
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6062..6081)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6116..6135
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6186..6321
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6341..6360)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6342..6360)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6370..6387)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6370..6386)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6379..6401)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6528..6983
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6615..6634)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6825..6846
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7009..7113
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7114..7182,7183..7974)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7332..7351)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8145..8733
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8634..8653
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8887..8904
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9021..9042
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9057..9062,9063..9080,9081..9087)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9095..9111
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9100..9122
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9119..9135
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9119..9135
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9154..9174
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9156..9174
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9202..9428
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1561 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     1621 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     1681 ttcaaaattt tatcgtccag tgtggtggaa ttgagtattc cagtgtggtg gaattctgca
     1741 gatatcaaca agtttgtaca aaaaagctga acgagaaacg taaaatgata taaatatcaa
     1801 tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat
     1861 ccagtcacta tggcggccgc attaggcacc ccaggcttta cactttatgc ttccggctcg
     1921 tataatgtgt ggattttgag ttaggatccg tcgagatttt caggagctaa ggaagctaaa
     1981 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa
     2041 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat
     2101 attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt
     2161 cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt
     2221 gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa
     2281 acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat
     2341 tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag
     2401 aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg
     2461 gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc
     2521 gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat
     2581 gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa
     2641 agatctggat ccggcttact aaaagccaga taacagtatg cgtatttgcg cgctgatttt
     2701 tgcggtataa gaatatatac tgatatgtat acccgaagta tgtcaaaaag aggtatgcta
     2761 tgaagcagcg tattacagtg acagttgaca gcgacagcta tcagttgctc aaggcatata
     2821 tgatgtcaat atctccggtc tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg
     2881 tgccgaacgc tggaaagcgg aaaatcagga agggatggct gaggtcgccc ggtttattga
     2941 aatgaacggc tcttttgctg acgagaacag gggctggtga aatgcagttt aaggtttaca
     3001 cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca
     3061 cgcccgggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtct
     3121 cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg
     3181 atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc agccaccgcg
     3241 aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg tcaggctccc
     3301 ttatacacag ccagtctgca ggtcgaccat agtgactgga tatgttgtgt tttacagtat
     3361 tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac
     3421 gtttctcgtt cagctttctt gtacaaagtg gttgatatcc agcacagtgg cggccgctcg
     3481 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     3541 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     3601 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     3661 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     3721 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     3781 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     3841 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     3901 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     3961 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     4021 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaattc
     4081 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg
     4141 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt
     4201 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta
     4261 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta
     4321 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc
     4381 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga
     4441 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg
     4501 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc
     4561 tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccacactc ctgcatctta
     4621 agcttgccac catggctcgg gaccctctgc cattctttcc acctctgtac ctgggcggcc
     4681 ctgagattac aaccgagaac tgcgagagag agcctatcca cattcctggg tccatccagc
     4741 cacacggggc tctgctcaca gctgacggcc actccggaga ggtgctccaa gtgtccctga
     4801 atgccgctac cttcctgggc caggagccta ctgtgctgcg ggggcagacc ctggctgccc
     4861 tgctccccga gcagtggcca gccctgcagg cagccctgcc cccaggatgt ccagatgccc
     4921 tccaatacag ggccaccctc gactggccag ctgctgggca cctcagcctg actgtgcatc
     4981 gggtggctga actcctgatc ctggagttcg aacctaccga ggcctgggac agcattggcc
     5041 ctcacgccct gaggaacgcc atgtttgctc tggaaagcgc tccaaacctg cgggctctgg
     5101 ccgaagtcgc aacacaaact gtgagagaac tgactggctt cgatcgggtg atgctgtaca
     5161 aatttgcccc tgacgccact ggagagatga ttgctgaggc cagacgggag ggcatgcagg
     5221 cttttctggg ccacaggttt cccgcatccc acacccctgc acaagctagg gccctctaca
     5281 caagacacct gctccggctg accgcagaca ccagggctgc agcagtgccc ctcgaccccg
     5341 tgctgaatcc ccagacaaat gctcctacac ctctgggcgg agctgtcctc agagctacat
     5401 ccccaatgca catgcagtac ctgaggaata tgggagtggg ctcctccctg agcgtcagcg
     5461 tcgtggtcgg cggccagctg tggggactga ttgtctgcca ccatcagaca ccctacgtgc
     5521 tgccaccaga tctgcggacc accctggagg agctggggag aaagctgtcc gggcaggtgc
     5581 agaggaaaga agccggaatg gacgagctgt ataaatgact cgaggacgcg tatgcatctc
     5641 gagggcccgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     5701 ttaaaagaaa aggggggact ggaagggcta gctcactccc aacgaagaca agatctgctt
     5761 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     5821 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     5881 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     5941 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     6001 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     6061 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     6121 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     6181 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     6241 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     6301 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta
     6361 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     6421 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     6481 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     6541 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     6601 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     6661 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     6721 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     6781 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     6841 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     6901 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     6961 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     7021 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     7081 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     7141 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     7201 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     7261 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     7321 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     7381 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     7441 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     7501 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     7561 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     7621 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     7681 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     7741 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     7801 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     7861 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     7921 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     7981 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     8041 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     8101 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     8161 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     8221 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     8281 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     8341 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     8401 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     8461 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     8521 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     8581 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     8641 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     8701 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     8761 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     8821 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     8881 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     8941 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     9001 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     9061 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     9121 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
     9181 aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg
     9241 taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg
     9301 tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga
     9361 ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat
     9421 acataaac
//
