LOCUS       sequence-347606-        9098 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral plasmid for GFP-TREX1 V235fs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9098)
  AUTHORS   Mohr L, Toufektchan E, von Morgen P, Chu K, Kapoor A, Maciejowski J
  TITLE     ER-directed TREX1 limits cGAS activation at micronuclei.
  JOURNAL   Mol Cell. 2021 Jan 12. pii: S1097-2765(20)30958-8. doi: 
            10.1016/j.molcel.2020.12.037.
  PUBMED    33476576
REFERENCE   2  (bases 1 to 9098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2021 Jan 12. pii: S1097-2765(20)30958-8. doi: 
            10.1016/j.molcel.2020.12.037."
COMMENT     Sequence Label: pLENTI CMV GFP-TREX1 V235fs BLAST
FEATURES             Location/Qualifiers
     source          1..9098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     238..258
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     misc_feature    309..897
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             415..426
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     824..844
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    complement(1648..1672)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(1682..2392,2393..2395,2396..2398))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(1682..2392,2393..2395,2396..2398))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1724..1745)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2073..2092
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2332..2353
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      2395..2404
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2473..2497)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(2494..2697)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2523..2543)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2698..3001
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    3024..3140
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(3407..3451)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3636..3869
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4362..4487
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4534..4714
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(4715..4941)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(4969..4989)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4969..4987)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5008..5024)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5008..5024)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5021..5043)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5032..5048
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5056..5062,5063..5080,5081..5086))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5101..5122
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5239..5256)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5410..5998)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5490..5509)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6169..6960,6961..7029))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6792..6811
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7030..7134)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7160..7615)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7297..7318)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7509..7528
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7742..7764
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7756..7773
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7757..7773
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7783..7802
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7783..7801
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      7822..7957
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7997..8118
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8008..8027)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8062..8081
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             8190..8423
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(8510..8908)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(8927..8974)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(join(9022..9098,1..253))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(9036..9098,1..73)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag
       61 ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca
      121 tgctttgcat acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact
      181 aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc
      241 ctaactgaca cacattccac agaattaatt ccaaactcat tactaaccgg atatctgcag
      301 aattccaggc ggggaggcgg cccaaaggga gatccgactc gtctgagggc gaaggcgaag
      361 acgcggaaga ggccgcagag ccggcagcag gccgcgggaa ggaaggtccg ctggattgag
      421 ggccgaaggg acgtagcaga aggacgtccc gcgcagaatc caggtggcaa cacaggcgag
      481 cagccatgga aaggacgtca gcttccccga caacaccacg gaattgtcag tgcccaacag
      541 ccgagcccct gtccagcagc gggcaaggca ggcggcgatg agttccgccg tggcaatagg
      601 gagggggaaa gcgaaagtcc cggaaaggag ctgacaggtg gtggcaatgc cccaaccagt
      661 gggggttgcg tcagcaaaca cagtgcacac cacgccacgt tgcctgacaa cgggccacaa
      721 ctcctcataa agagacagca accaggattt atacaaggag gagaaaatga aagccatacg
      781 ggaagcaata gcatgataca aaggcattaa agcagcgtat ccacatagcg taaaaggagc
      841 aacatagtta agaataccag tcaatctttc acaaattttg taatccagag gttgattgtc
      901 gacgcggccg ctttacttca ccggtgtcct agcagaggct gtgaccccca tacatgggcc
      961 tgatggtgcc gaaaggcctg gcgtgagcat ccacccaccg cagcagggcc tgtggtctcc
     1021 actgacagat gctgagcagg gccaggacat caccctcagc cgtgtgcgag tctggagggg
     1081 actgcccata caggcgagtg tagatgctgc ctaggctata gctcttcctt gggccgtgtt
     1141 ctgaggggct gcttgctcgc tccagggcct tcagcgcagt gatgctatcc acacagaagg
     1201 caccatccag agcactggtg aggcccagca tagccagctc tgcttggagc agggggaagt
     1261 cgtagcggtc accattgtgt gccaccaggc accagggctg tggctggcgc cgcaggaagg
     1321 ctaggagcag gttggccagg ttgtcatcaa aacattgacg cccatgcgct gccagcacag
     1381 ctgtgctcag acctgtgatc tcgctggctg cagggctgca ggccttcccc ggagccacac
     1441 acagggagag cttgtctacc acacgcggtg gtggaggaac tgtgggaggt ggcccctgag
     1501 aggtgggggg gctctccagg gcacatctgt ggacagccag caggcacagc tccgtgacct
     1561 tgggctggga gaagggcaag ccagtggcct ccatgtcgaa aaagatgagg gtctgcatgg
     1621 gccccggggg cagggcctgc gagccccagc ctgctttttt gtacaaactt gttgatccgg
     1681 tcttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga
     1741 ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc ggactgggtg ctcaggtagt
     1801 ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg
     1861 cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt
     1921 tcttctgctt gtcggccatg atatagacgt tgtggctgtt gtagttgtac tccagcttgt
     1981 gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca
     2041 gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga
     2101 agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct
     2161 tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg gtcacgaggg
     2221 tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt
     2281 aggtggcatc gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt
     2341 ccagctcgac caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatgg
     2401 tggcgaccgg tggatcctct agagtcggtg tcttctatgg aggtcaaaac agcgtggatg
     2461 gcgtctccag gcgatctgac ggttcactaa acgagctctg cttatataga cctcccaccg
     2521 tacacgccta ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc
     2581 ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga gacttggaaa
     2641 tccccgtgag tcaaaccgct atccacgccc attgatgtac tgccaaaacc gcatcaccat
     2701 ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc ataaggtcat
     2761 gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag ggggcgtact
     2821 tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat actccaccca
     2881 ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca ttattgacgt
     2941 caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac
     3001 gcggaactcc caagcttatc gataaaattt tgaattttgt aatttgtttt tgtaattctt
     3061 tagtttgtat gtctgttgct attatgtcta ctattctttc ccctgcactg taccccccaa
     3121 tccccccttt tcttttaaaa gttaaccgat accgtcgaga tccgttcact aatcgaatgg
     3181 atctgtctct gtctctctct ccaccttctt cttctattcc ttcgggcctg tcgggtcccc
     3241 tcggggttgg gaggtgggtc tgaaacgata atggtgaata tccctgccta actctattca
     3301 ctatagaaag tacagcaaaa actattctta aacctaccaa gcctcctact atcattatga
     3361 ataattttat ataccacagc caatttgtta tgttaaacca attccacaaa cttgcccatt
     3421 tatctaattc caataattct tgttcattct tttcttgctg gttttgcgat tcttcaatta
     3481 aggagtgtat taagcttgtg taattgttaa tttctctgtc ccactccatc caggtcgtgt
     3541 gattccaaat ctgttccaga gatttattac tccaactagc attccaaggc acagcagtgg
     3601 tgcaaatgag ttttccagag caaccccaaa tccccaggag ctgttgatcc tttaggtatc
     3661 tttccacagc caggattctt gcctggagct gcttgatgcc ccagactgtg agttgcaaca
     3721 gatgctgttg cgcctcaata gccctcagca aattgttctg ctgctgcact ataccagaca
     3781 ataattgtct ggcctgtacc gtcagcgtca ttgacgctgc gcccatagtg cttcctgctg
     3841 ctcccaagaa cccaaggaac aaagctccta ttcccactgc tcttttttct ctctgcacca
     3901 ctcttctctt tgccttggtg ggtgctactc ctaatggttc aatttttact actttatatt
     3961 tatataattc acttctccaa ttgtccctca tatctcctcc tccaggtctg aagatcagcg
     4021 gccgcttgct gtgcggtggt cttacttttg ttttgctctt cctctatctt gtctaaagct
     4081 tccttggtgt cttttatctc tatcctttga tgcacacaat agagggttgc tactgtatta
     4141 tataatgatc taagttcttc tgatcctgtc tgaagggatg gttgtagctg tcccagtatt
     4201 tgtctacagc cttctgatgt ttctaacagg ccaggattaa ctgcgaatcg ttctagctcc
     4261 ctgcttgccc atactatatg ttttaattta tattttttct ttccccctgg ccttaaccga
     4321 attttttccc atcgcgatct aattctcccc cgcttaatac tgacgctctc gcacccatct
     4381 ctctccttct agcctccgct agtcaaaatt tttggcgtac tcaccagtcg ccgcccctcg
     4441 cctcttgccg tgcgcgcttc agcaagccga gtcctgcgtc gagagagctc ctctggtttc
     4501 cctttcgctt tcaagtccct gttcgggcgc cactgctaga gattttccac actgactaaa
     4561 agggtctgag ggatctctag ttaccagagt cacacaacag acgggcacac actacttgaa
     4621 gcactcaagg caagctttat tgaggcttaa gcagtgggtt ccctagttag ccagagagct
     4681 cccaggctca gatctggtct aaccagagag acccgtttat gtatcgagct aggcacttaa
     4741 atacaatatc tctgcaatgc ggcaattcag tggttcgtcc aatccatgtc agacccgtct
     4801 gttgccttcc taataaggca cgatcgtacc accttacttc caccaatcgg catgcacggt
     4861 gctttttctc tccttgtaag gcatgttgct aactcatcgt taccatgttg caagactaca
     4921 agagtattgc ataagactac attaagcttg cagctccagc ttttgttccc tttagtgagg
     4981 gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     5041 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     5101 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     5161 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     5221 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     5281 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     5341 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     5401 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     5461 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     5521 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     5581 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     5641 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     5701 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     5761 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     5821 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     5881 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     5941 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     6001 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     6061 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     6121 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     6181 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     6241 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     6301 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     6361 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     6421 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     6481 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     6541 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     6601 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     6661 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     6721 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     6781 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     6841 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     6901 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     6961 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     7021 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     7081 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     7141 tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
     7201 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     7261 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     7321 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     7381 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     7441 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     7501 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     7561 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat
     7621 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     7681 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     7741 tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag
     7801 ggcgaattgg gtacgtccct aggcctccaa aaaagcctcc tcactacttc tggaatagct
     7861 cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg
     7921 gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggatag
     7981 ctagagccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     8041 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     8101 agctgcaata aacaagttcc tctcactctc tgatattcat ttctttgcaa gttataaata
     8161 ctgaataata agatgacatg aactactact gctagagatt ttccacactg actaaaaggg
     8221 tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta cttgaagcac
     8281 tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     8341 ggctcagatc tggtctaacc agagagaccc agtacaagca aaaagcagat cttgtcttcg
     8401 ttgggagtga gctagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     8461 agctgccttg taagtcattg gtcttaaagg taccgagctc gaattgtgct tagccctccc
     8521 acacataacc agagggcagc aattcacgaa tcccaactgc cgtcggctgt ccatcactgt
     8581 ccttcactat ggctttgatc ccaggatgca gatcgagaag cacctgtcgg caccgtccgc
     8641 aggggctcaa gatgcccctg ttctcatttc cgatcgcgac gatacaagtc aggttgccag
     8701 ctgccgcagc agcagcagtg cccagcacca cgagttctgc acaaggtccc ccagtaaaat
     8761 gatatacatt gacaccagtg aagatgcggc cgtcgctaga gagagctgcg ctggcgacgc
     8821 tgtagtcttc agagatgggg atgctgttga ttgtagccgt tgctctttca atgagggtgg
     8881 attcttcttg agacaaaggc ttggccatgg tttagttcct caccttgtcg tattatacta
     8941 tgccgatata ctatgccgat gattaattgt caacacgtgc tgatcagatc cgaaaatgga
     9001 tatacaagct cccgggagct ttttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta
     9061 cttctggaat agctcagagg cagaggcggc ctcggcct
//
