LOCUS       sequence-347643-       10312 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses 3X-HA-AGO1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10312)
  AUTHORS   Muller M, Fah T, Schaefer M, Hermes V, Luitz J, Stalder P, Arora R, 
            Ngondo RP, Ciaudo C
  TITLE     AGO1 regulates pericentromeric regions in mouse embryonic stem cells
  JOURNAL   Life Sci Alliance. 2022 Mar 2;5(6). pii: 5/6/e202101277. doi: 
            10.26508/lsa.202101277. Print 2022 Jun.
  PUBMED    35236760
REFERENCE   2  (bases 1 to 10312)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2022-03-2"; volume: "5(6). pii: 5/6/e202101277. 
            doi"; pages: " 10.26508/lsa.202101277. Print 2022 Jun"
COMMENT     Sequence Label: pMSCV_PIG_3XHA-Ago1
FEATURES             Location/Qualifiers
     source          1..10312
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     37..56
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             125..724
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(125..144)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     621..641
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    785..1352
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    802..1354
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(946..963)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1173..1192
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(1366..1368,1369..1371,1372..2085)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1411..1432)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1672..1691)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2019..2040
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             2187..2701
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    2863..2879
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2887..2893,2894..2911,2912..2917))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2932..2953
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3070..3087)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3241..3829)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3321..3340)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4000..4791,4792..4860))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4623..4642
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4861..4965)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5033..5051
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5089..5111)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5211..5230
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5424..5446
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5574..5593
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             5793..6309
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    6373..6714
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             6781..7197
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     7127..7149
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     7165..7181
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             7235..7324
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAASYPYDVPDYA"
     promoter        join(9917..10312,1..104)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9952..9975)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca
       61 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcccaagct
      121 taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
      181 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
      241 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
      301 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
      361 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
      421 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
      481 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
      541 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
      601 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
      661 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
      721 ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc
      781 caattccgcc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
      841 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
      901 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
      961 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
     1021 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
     1081 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
     1141 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
     1201 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca
     1261 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg
     1321 acgtggtttt cctttgaaaa acacgatgat aatatggcca caaccatggt gagcaagggc
     1381 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     1441 cacaagttca gcgtgtctgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     1501 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     1561 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     1621 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     1681 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     1741 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     1801 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggcgaac
     1861 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     1921 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     1981 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     2041 accgccgccg ggatcactct cggcatggac gagctgtaca agtaatgaat taattaagaa
     2101 ttatcaagct tatcgatacc gtcgacctgc agccaagctt atcgataaaa taaaagattt
     2161 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag
     2221 cttaagtaac gccattttgc aaggcatgga aaatacataa ctgagaatag agaagttcag
     2281 atcaaggtta ggaacagaga gacagcagaa tatgggccaa acaggatatc tgtggtaagc
     2341 agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc ccgccctcag
     2401 cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt
     2461 gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc
     2521 cgagctcaat aaaagagccc acaacccctc actcggcgcg ccagtcctcc gatagactgc
     2581 gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct
     2641 cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca
     2701 tgggtaacag tttcttgaag ttggagaaca acattctgag ggtaggagtc gaatattaag
     2761 taatcctgac tcaattagcc actgttttga atccacatac tccaatactc ctgaaatagt
     2821 tcattatgga cagcgcagaa agagctgggg agaattgtga aattgttatc cgctcacaat
     2881 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     2941 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     3001 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     3061 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     3121 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     3181 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     3241 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     3301 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     3361 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     3421 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     3481 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     3541 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     3601 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     3661 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     3721 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     3781 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     3841 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     3901 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     3961 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     4021 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     4081 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     4141 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     4201 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     4261 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     4321 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     4381 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     4441 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     4501 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     4561 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     4621 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     4681 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     4741 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     4801 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     4861 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     4921 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     4981 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     5041 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     5101 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     5161 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
     5221 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
     5281 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
     5341 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
     5401 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg cgcaaggaat
     5461 ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc
     5521 acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg
     5581 tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt
     5641 ccggcgtaga ggcgattagt ccaatttgtt aaagacagga tatcagtggt ccaggctcta
     5701 gttttgactc aacaatatca ccagctgaag cctatagagt acgagccata gataaaataa
     5761 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
     5821 aagctagctt aagtaacgcc attttgcaag gcatggaaaa tacataactg agaatagaga
     5881 agttcagatc aaggttagga acagagagac agcagaatat gggccaaaca ggatatctgt
     5941 ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtcccg
     6001 ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg
     6061 accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc
     6121 tgctccccga gctcaataaa agagcccaca acccctcact cggcgcgcca gtcctccgat
     6181 agactgcgtc gcccgggtac ccgtattccc aataaagcct cttgctgttt gcatccgaat
     6241 cgtggactcg ctgatccttg ggagggtctc ctcagattga ttgactgccc acctcggggg
     6301 tctttcattt ggaggttcca ccgagatttg gagacccctg cccagggacc accgaccccc
     6361 ccgccgggag gtaagctggc cagcggtcgt ttcgtgtctg tctctgtctt tgtgcgtgtt
     6421 tgtgccggca tctaatgttt gcgcctgcgt ctgtactagt tagctaacta gctctgtatc
     6481 tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc tgggagacgt
     6541 cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc gatgtggaat
     6601 ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca gttcccgcct
     6661 ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct gctgcagcgc
     6721 tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc tgaaaattag
     6781 ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga tgtcgagcgg
     6841 atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt ctgctctgca
     6901 gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg agacctcatc
     6961 acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga ccaggtcccc
     7021 tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa gccctttgta
     7081 caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct tgaacctcct
     7141 cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct aggcgccgga
     7201 attagatctc tcgaggttaa cgaattccac catgtaccca tacgatgttc ctgactatgc
     7261 gggctatccc tatgacgtcc cggactatgc agcatcctat ccatatgacg ttccagatta
     7321 cgctggatcc gaagcgggac cctcgggagc agcggcaggt gcctacctgc ctcccctgca
     7381 gcaggtgttt caggcacccc gccggcctgg cattggcact gtgggcaaac caatcaagct
     7441 tctggccaat tactttgagg tggacattcc taagattgac gtttaccatt acgaggtgga
     7501 catcaagccg gataagtgtc ctcgcagagt caaccgggag gtggtggaat acatggtcca
     7561 gcatttcaaa ccgcagatct ttggggatcg caagcctgtg tatgatggaa agaagaatat
     7621 ttacacggtc acagcactgc ccattggcaa tgagagggtt gactttgagg tgacaatccc
     7681 cggggagggg aaggatagaa tttttaaggt ctccatcaag tggctagcca ttgtgagctg
     7741 gcgcatgctg catgaagcct tggtcagtgg ccagatccct gtgcccctgg agtctgtgca
     7801 agccctggat gtggccatga ggcacctggc atctatgagg tacacccctg tgggccgctc
     7861 cttcttctca ccgcctgagg gctactacca cccgctgggg ggtgggcgcg aggtctggtt
     7921 cggctttcac cagtctgtgc gccctgccat gtggaagatg atgctcaaca ttgatgtctc
     7981 agccactgcc ttctacaaag cacagccagt gattgagttc atgtgtgagg tcctggacat
     8041 caggaacata gacgaacaac ccaagcccct cacggattcc cagcgtgttc ggtttaccaa
     8101 ggagataaaa ggcctgaagg tggaagtgac ccactgtgga cagatgaaga ggaaataccg
     8161 cgtgtgtaat gttacccgcc gccctgctag ccatcagacg ttccccttgc agctggagag
     8221 tggacagact gtggagtgca ctgtggcaca gtatttcaag cagaaatata accttcagct
     8281 caagtatcct cacctgccct gcctacaagt tgggcaagaa cagaagcata cctatttgcc
     8341 cctcgaggtc tgtaacattg tggctgggca gcggtgcatt aagaagctga ctgacaacca
     8401 gacttcaacc atgataaagg ctacagctag gtcggcccca gacagacagg aggagatcag
     8461 ccgcctgatg aagaatgcta gctacaacct ggatccctac atccaggaat ttggaatcaa
     8521 agtgaaggat gacatgacgg aggtgacagg acgtgtgctg ccggcgccca tcttgcagta
     8581 tggcggccgg aaccgggcca ttgctacgcc caaccaaggt gtctgggaca tgcgcgggaa
     8641 acagttctac aatgggattg agatcaaagt ctgggccatc gcctgcttcg caccccagaa
     8701 acagtgtcga gaagaggtgc tcaagaactt cacagaccag cttcggaaga tttccaagga
     8761 tgcagggatg cccatccagg gtcagccatg cttctgcaaa tacgcacagg gggcagacag
     8821 tgtggagccc atgttccggc atctcaagaa cacctactca ggactgcagc tcattatcgt
     8881 catcctgccc gggaagacgc cagtgtatgc tgaagtgaaa cgtgtcggag atacactctt
     8941 gggaatggca acacagtgtg tgcaggtgaa gaatgtggtc aagacgtcac cgcagactct
     9001 gtccaacctc tgcctcaaga tcaatgtcaa actcggtggc attaacaaca tcctagtccc
     9061 acaccagcgc tctgctgttt ttcaacagcc agtgattttc ctgggagcag atgttacaca
     9121 tcccccagct ggggatggga agaaaccatc tatcacagca gtggtaggca gcatggacgc
     9181 acaccccagc cgatactgtg ccactgtgcg tgtgcagcgc ccacggcagg agatcattga
     9241 agacttgtcc tatatggtgc gcgagctgct tatccagttc tacaagtcca cccgcttcaa
     9301 gcccacccgc atcatcttct accgcgacgg cgtccccgag ggccagctgc cccagattct
     9361 tcactatgag ctgcttgcca ttcgagatgc atgcatcaaa ctggaaaaag actaccagcc
     9421 tgggatcaca tatattgtgg tgcagaagcg ccatcacacc cgcctgttct gtgctgacaa
     9481 gaatgagcgg attgggaaga gtggtaacat cccagcaggg accactgtgg acaccaacat
     9541 cacccaccca tttgagttcg acttctatct gtgcagccat gcaggcatcc agggtaccag
     9601 ccgaccatcc cattattatg tcctttggga tgacaaccgt ttcacagcag atgaactcca
     9661 gatcttgaca taccagctgt gccacactta cgtacgatgc acacgttctg tctctatccc
     9721 agcacctgcc tactatgctc gcttggtggc tttccgggca cgataccacc tggtggacaa
     9781 ggaacatgac agtggagagg ggagccacat atcagggcaa agcaatgggc gagaccccca
     9841 ggccctggcc aaagctgtgc aggttcacca ggatactcta cgcaccatgt acttcgcttg
     9901 aggatccaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct
     9961 ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc
    10021 acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct
    10081 actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga
    10141 caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg
    10201 aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct
    10261 cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gg
//
