LOCUS       sequence-34765-e         681 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 681)
  TITLE     Cookson Lab Plasmids
REFERENCE   2  (bases 1 to 681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDEST51-LRRK2-G2019S
FEATURES             Location/Qualifiers
     source          1..681
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(2..19)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(29..70)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    96..120
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 catggtgatg gtgatgatga ccggtacgcg tagaatcgag accgaggaga gggttaggga
       61 taggcttacc ttcgaaccgc gggccctcta gatcaaccac tttgtacaag aaagctgggt
      121 cgaattcgcc ctttttctca acagatgttc gtctcatttt ttcagctaat tcttttctca
      181 cttcaatgtg tttttctaaa ttttgcactt catgtggaag attgatgtcc caaacggtca
      241 agcaagattg tatctctttc tgcttttgtg taccttcagt atttttccgg ttgtagccca
      301 ataccagcat gacattttta aggcttccta gctgtgctgt catcatgact ctgaccgaat
      361 tacaaaagtt gtaaattaca cgtataagtc gacgagttga aagatccagg agtaaaatat
      421 ggcctcctcc agttcctatc caaagagcag tgttcttctg aaggcagagg gttttcactc
      481 tcccagaata agacattttg tgttttgatt ccttgttttc ttttaccatt acctccctta
      541 aaaagtgcac gcagtctatt agtccacaga gtttttcagt tttcttatcc cacacttcca
      601 caacagggct attttgctta gcaatataga gagcagtgtc taccaccact gttatgatgt
      661 tggaatcact gaaagctgca t
//
