LOCUS       sequence-347656-        1100 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasma membrane-targeted variant of improved green-red FRET-based 
            PKA activity reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Mo GCH, Posner C, Rodriguez EA, Sun T, Zhang J
  TITLE     A rationally enhanced red fluorescent protein expands the utility of
            FRET biosensors.
  JOURNAL   Nat Commun. 2020 Apr 15;11(1):1848. doi: 10.1038/s41467-020-15687-x.
  PUBMED    32296061
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-04-15"; volume: "11(1):1848. doi"; pages: " 
            10.1038/s41467-020-15687-x"
COMMENT     Sequence Label: pcDNA3-Lyn-GR-AKAR3
FEATURES             Location/Qualifiers
     source          1..1100
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gap             6..19
                     /estimated_length=14
     primer_bind     complement(874..895)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gggngnnnnn nnnnnnnnnc tcgcnntgag ctcgctgccg ccgtcaacca gcgtcgcgcg
       61 cctcaatccg gaaccaccag cactaccacc agcactacca ccagcactac caccagcact
      121 accaccagca ctgccggaac caccagcact accaccagca ctaccaccag cactaccacc
      181 agcactacca ccagcactgg tactaccacc agcactacca ccagcactgc cggaaccacc
      241 agcactacca ccagcactac caccagcact accaccagca ctaccaccag cactggtact
      301 accaccagca ctaccaccag cactgccgga accaccagca ctaccaccag cactaccacc
      361 agcactacca ccagcactac caccagcact ggtaccgcga tcaactttgt tctgctccag
      421 gcactgctta aatttgtcgt ttatgaaaat gaccagagat aaaatatctg attccacgcc
      481 tacaccaacg gttatttcat caccttggga cagtaactga ttgctgttct tctcgacttt
      541 ttgcccattt aaccaggtcc cattagtgga aatgtcattc aataaaaggt taccgtcttc
      601 tcctagtagt atttggaaat gcttatttga cagtcttgaa atgtttccta aatgatagtc
      661 acaggctggg tttctaccaa atgtccaaac tttctttatg gatcgttttt ccttaagcac
      721 ttgtgaaata tcagctgaca aatctcggat gggaatttga cccgtggtac aaatgaccct
      781 gcacacaatg ttttcgccga tctgttcttg agaaaactta tgcatagatc tcttgtacag
      841 ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga ccatgtgatc
      901 gcgcttctcg ttggggtctt tgctcagggc ggactgggtg ctcaggtagt ggntngtcgg
      961 gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtngncg ncganctgca
     1021 cgctgccgtc ctcgatgnng tngnggatct tgaagtnggc cttgatgccg tnnttctgct
     1081 tgtcggncat gatannnnna
//
