LOCUS       sequence-347659-        9248 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Custom vector for sgRNA library construction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9248)
  AUTHORS   Hussmann JA, Ling J, Ravisankar P, Yan J, Cirincione A, Xu A, 
            Simpson D, Yang D, Bothmer A, Cotta-Ramusino C, Weissman JS, Adamson
            B
  TITLE     Mapping the genetic landscape of DNA double-strand break repair.
  JOURNAL   Cell. 2021 Oct 28;184(22):5653-5669.e25. doi: 
            10.1016/j.cell.2021.10.002. Epub 2021 Oct 20.
  PUBMED    34672952
REFERENCE   2  (bases 1 to 9248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.10.002"; journalName: "Cell"; date: 
            "2021-10-28- 28"; volume: "184"; issue: "22"; pages: "5653-5669.e25"
COMMENT     Sequence Label: pAX198
FEATURES             Location/Qualifiers
     source          1..9248
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(37..625)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(117..136)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(796..1587,1588..1656))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1419..1438
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1657..1761)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1836..1855)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2027..2406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2408..2606
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2561..2581
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2624..2804
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2851..2976
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3473..3706
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3891..3935
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4233..4350
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(4603..4636)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     4640..4660
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        5276..5487
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          5488..6426
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6383..6403
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             6447..7043
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6447..6466)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6943..6963
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             7053..7106
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7119..7817
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     protein_bind    complement(7837..7870)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    7926..8514
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7979..7999)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8397..8408)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8517..8533)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8518..8534)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9043..9223
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
       61 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
      121 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
      181 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
      241 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
      301 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
      361 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
      421 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
      481 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
      541 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
      601 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
      661 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
      721 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
      781 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
      841 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
      901 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
      961 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     1021 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     1081 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     1141 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     1201 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     1261 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     1321 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     1381 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     1441 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     1501 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     1561 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     1621 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     1681 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     1741 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
     1801 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
     1861 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
     1921 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
     1981 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg
     2041 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     2101 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     2161 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     2221 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     2281 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     2341 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     2401 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     2461 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     2521 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     2581 gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga
     2641 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     2701 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     2761 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     2821 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     2881 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     2941 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     3001 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     3061 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     3121 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     3181 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     3241 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     3301 accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     3361 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     3421 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     3481 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     3541 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     3601 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     3661 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     3721 gctctggaaa actcatttgc accactgcgg ccgcttggaa tgctagttgg agtaataaat
     3781 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     3841 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     3901 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     3961 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     4021 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     4081 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     4141 gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa
     4201 ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     4261 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     4321 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     4381 gtttggttag taccgggccc gctctagaga tccgacgcgc catctctagg cccgcgccgg
     4441 ccccctcgca cggacttgtg ggagaagctc ggctactccc ctgccccggt taatttgcat
     4501 ataatatttc ctagtaacta tagaggctta atgtgcgata aaagacagat aatctgttct
     4561 ttttaatact agctacattt tacatgatag gcttggattt ctataacttc gtatagcata
     4621 cattatacga agttataaac agcacaaaag gaaactcacc ctaactgtaa agtaattgtg
     4681 tgttttgaga ctataagtat cccttggaga accaccttgt tggaccagga tgggcaccac
     4741 ccgtttaaga gctaagctgg aaacagcata gcaagtttaa ataaggctag tccgttatca
     4801 acttgaaaaa gtggcaccga gtcggtgctt ttttctcgag cactcctgat gctgttatgg
     4861 gcaaccctaa ggtgaaggct catggcaaga aagtgctcgg tgcctttagt gatggcctgg
     4921 ctcacctgga caacctcaag ggcacctttg ccacactgag tgagctgcac tgtgacaagc
     4981 tgcacgtaga tcctgagaac ttcaggctcc tgggcaacgt gctggtctgt gtgctggcct
     5041 atcactttgg caaagaattc accccaccag tgcaggctgc ctatcagaaa gtggtggctg
     5101 gtgtggctaa tgccctggcc cacaagtatc actaagctcg ctttcttgct gtccaatttc
     5161 tattaaaggt tcctttgttg catgctacgt gcagcggccg cctaggatcc atgccgatag
     5221 cgttggttga gtggataacc gtattaccgc caagcttatg catgtgcccg tcagtgggca
     5281 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt
     5341 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
     5401 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
     5461 cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc
     5521 ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg
     5581 attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa
     5641 ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg
     5701 cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa
     5761 aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc
     5821 caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg
     5881 tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg
     5941 gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc
     6001 ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg
     6061 cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg
     6121 ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac
     6181 tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgcgctt ttggagtacg
     6241 tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg
     6301 gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt
     6361 gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca
     6421 tttcaggtgt cgtgagctag cccaccatga ccgagtacaa gcccacggtg cgcctcgcca
     6481 cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     6541 ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     6601 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     6661 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     6721 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     6781 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     6841 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     6901 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     6961 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     7021 gcatgacccg caagcccggt gccggatcgg gagagggcag aggaagtctg ctaacatgcg
     7081 gtgacgtcga ggagaatcct ggcccaccgg tcgccaccag cgagctgatt aaggagaaca
     7141 tgcacatgaa gctgtacatg gagggcaccg tggacaacca tcacttcaag tgcacatccg
     7201 agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggtg gtcgagggcg
     7261 gccctctccc cttcgccttc gacatcctgg ctactagctt cctctacggc agcaagacct
     7321 tcatcaacca cacccagggc atccccgact tcttcaagca gtccttccct gagggcttca
     7381 catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca
     7441 gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac ttcacatcca
     7501 acggccctgt gatgcagaag aaaacactcg gctgggaggc cttcaccgag acgctgtacc
     7561 ccgctgacgg cggcctggaa ggcagaaacg acatggccct gaagctcgtg ggcgggagcc
     7621 atctgatcgc aaacatcaag accacatata gatccaagaa acccgctaag aacctcaaga
     7681 tgcctggcgt ctactatgtg gactacagac tggaaagaat caaggaggcc aacaacgaga
     7741 cctacgtcga gcagcacgag gtggcagtgg ccagatactg cgacctccct agcaaactgg
     7801 ggcacaagct taattaagaa ttcgtcgagg gacctaataa cttcgtatag catacattat
     7861 acgaagttat acatgtttaa gggttccggt tccactaggt acaattcgat atcaagctta
     7921 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     7981 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     8041 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     8101 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     8161 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     8221 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     8281 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     8341 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     8401 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     8461 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
     8521 accgtcgacc tcgatcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca
     8581 gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca
     8641 gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     8701 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatatc
     8761 cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa ctacacacca
     8821 gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
     8881 caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca ccctgtgagc
     8941 ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga cagccgccta
     9001 gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct ctggttagac
     9061 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     9121 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     9181 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagcatgtg agcaaaaggc
     9241 cagcaaaa
//
