LOCUS       sequence-347661-       11404 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses wildtype MRE11 in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11404)
  AUTHORS   Hussmann JA, Ling J, Ravisankar P, Yan J, Cirincione A, Xu A, 
            Simpson D, Yang D, Bothmer A, Cotta-Ramusino C, Weissman JS, Adamson
            B
  TITLE     Mapping the genetic landscape of DNA double-strand break repair.
  JOURNAL   Cell. 2021 Oct 28;184(22):5653-5669.e25. doi: 
            10.1016/j.cell.2021.10.002. Epub 2021 Oct 20.
  PUBMED    34672952
REFERENCE   2  (bases 1 to 11404)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.10.002"; journalName: "Cell"; date: 
            "2021-10-28- 28"; volume: "184"; issue: "22"; pages: "5653-5669.e25"
COMMENT     Sequence Label: pJL056
FEATURES             Location/Qualifiers
     source          1..11404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             89..722
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    769..894
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1391..1624
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1809..1853
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2146..2263
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2327..3505
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2558..3496
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3453..3473
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5664..5690
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        5776..6987
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     6824..6843
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(7001..7003,7004..7717)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             7001..7714
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(7031..7059)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     7654..7674
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     misc_feature    7882..8470
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7935..7955)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8353..8364)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8545..8778
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8897..8915)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8983..9087
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9088..9156,9157..9948)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9306..9325)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10119..10707
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10608..10627
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10861..10878
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11118..11139
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11154..11159,11160..11177,11178..11184)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11192..11208
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11197..11219
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11216..11232
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11216..11232
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11282..11301
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
       61 gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa
      121 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac
      181 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca
      241 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct
      301 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc
      361 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga
      421 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc
      481 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac
      541 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
      601 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
      661 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
      721 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
      781 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
      841 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
      901 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
      961 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     1021 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     1081 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     1141 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     1201 caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag
     1261 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     1321 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     1381 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     1441 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     1501 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     1561 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     1621 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     1681 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     1741 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     1801 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
     1861 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
     1921 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
     1981 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
     2041 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
     2101 ctcgacggta tcgccgaatt cacaaatggc agtattcatc cacaatttta aaagaaaagg
     2161 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2221 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2281 cagcagagat ccagtttgga ctaggatccc cagtttggtt agtaccgggc ccggtgcccg
     2341 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
     2401 ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
     2461 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
     2521 cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg
     2581 cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct
     2641 gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc
     2701 ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg
     2761 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
     2821 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt
     2881 aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg
     2941 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
     3001 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc
     3061 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
     3121 aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg
     3181 agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc
     3241 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgcgctt
     3301 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
     3361 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
     3421 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
     3481 ttttcttcca tttcaggtgt cgtgagttaa ccatgagtac tgcagatgca cttgatgatg
     3541 aaaacacatt taaaatatta gttgcaacag atattcatct tggatttatg gagaaagatg
     3601 cagtcagagg aaatgatacg tttgtaacac tcgatgaaat tttaagactt gcccaggaaa
     3661 atgaagtgga ttttattttg ttaggtggtg atctttttca tgaaaataag ccctcaagga
     3721 aaacattaca tacctgcctc gagttattaa gaaaatattg tatgggtgat cggcctgtcc
     3781 agtttgaaat tctcagtgat cagtcagtca actttggttt tagtaagttt ccatgggtga
     3841 actatcaaga tggcaacctc aacatttcaa ttccagtgtt tagtattcat ggcaatcatg
     3901 acgatcccac aggggcagat gcactttgtg ccttggacat tttaagttgt gctggatttg
     3961 taaatcactt tggacgttca atgtctgtgg agaagataga cattagtccg gttttgcttc
     4021 aaaaaggaag cacaaagatt gcgctatatg gtttaggatc cattccagat gaaaggctct
     4081 atcgaatgtt tgtcaataaa aaagtaacaa tgttgagacc aaaggaagat gagaactctt
     4141 ggtttaactt atttgtgatt catcagaaca ggagtaaaca tggaagtact aacttcattc
     4201 cagaacaatt tttggatgac ttcattgatc ttgttatctg gggccatgaa catgagtgta
     4261 aaatagctcc aaccaaaaat gaacaacagc tgttttatat ctcacaacct ggaagctcag
     4321 tggttacttc tctttcccca ggagaagctg taaagaaaca tgttggtttg ctgcgtatta
     4381 aagggaggaa gatgaatatg cataaaattc ctcttcacac agtgcggcag tttttcatgg
     4441 aggatattgt tctagctaat catccagaca tttttaaccc agataatcct aaagtaaccc
     4501 aagccataca aagcttctgt ttggagaaga ttgaagaaat gcttgaaaat gctgaacggg
     4561 aacgtctggg taattctcac cagccagaga agcctcttgt acgactgcga gtggactata
     4621 gtggaggttt tgaacctttc agtgttcttc gctttagcca gaaatttgtg gatcgggtag
     4681 ctaatccaaa agacattatc cattttttca ggcatagaga acaaaaggaa aaaacaggag
     4741 aagagatcaa ctttgggaaa cttatcacaa agccttcaga aggaacaact ttaagggtag
     4801 aagatcttgt aaaacagtac tttcaaaccg cagagaagaa tgtgcagctc tcactgctaa
     4861 cagaaagagg gatgggtgaa gcagtacaag aatttgtgga caaggaggag aaagatgcca
     4921 ttgaggaatt agtgaaatac cagttggaaa aaacacagcg atttcttaaa gaacgtcaca
     4981 ttgatgccct cgaagacaaa atcgatgagg aggtacgtcg tttcagagaa accagacaaa
     5041 aaaatactaa tgaagaagat gatgaagtcc gtgaggctat gaccagggcc agagcactca
     5101 gatctcagtc agaggagtct gcttctgcct ttagtgctga tgaccttatg agtatagatt
     5161 tagcagaaca gatggctaat gactctgatg atagcatctc agcagcaacc aacaaaggaa
     5221 gaggccgagg aagaggtcga agaggtggaa gagggcagaa ttcagcatcg agaggagggt
     5281 ctcaaagagg aagagcagac actggtctgg agacttctac ccgtagcagg aactcaaaga
     5341 ctgctgtgtc agcatctaga aatatgtcta ttatagatgc ctttaaatct acaagacagc
     5401 agccttcccg aaatgtcact actaagaatt attcagaggt gattgaggta gatgaatcag
     5461 atgtggaaga agacattttt cctaccactt caaagacaga tcaaaggtgg tccagcacat
     5521 catccagcaa aatcatgtcc cagagtcaag tatcgaaagg ggttgatttt gaatcaagtg
     5581 aggatgatga tgatgatcct tttatgaaca ctagttcttt aagaagaaat agaagataaa
     5641 atctagaggg cccgcggttc gaatacccat acgacgtccc agactacgct tagtaatgat
     5701 taattaaact agaaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagta
     5761 acccacccaa gatctggcct ccgcgccggg ttttggcgcc tcccgcgggc gcccccctcc
     5821 tcacggcgag cgctgccacg tcagacgaag ggcgcagcga gcgtcctgat ccttccgccc
     5881 ggacgctcag gacagcggcc cgctgctcat aagactcggc cttagaaccc cagtatcagc
     5941 agaaggacat tttaggacgg gacttgggtg actctagggc actggttttc tttccagaga
     6001 gcggaacagg cgaggaaaag tagtcccttc tcggcgattc tgcggaggga tctccgtggg
     6061 gcggtgaacg ccgatgatta tataaggacg cgccgggtgt ggcacagcta gttccgtcgc
     6121 agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg tgatcgtcac ttggtgagta
     6181 gcgggctgct gggctggccg gggctttcgt ggccgccggg ccgctcggtg ggacggaagc
     6241 gtgtggagag accgccaagg gctgtagtct gggtccgcga gcaaggttgc cctgaactgg
     6301 gggttggggg gagcgcagca aaatggcggc tgttcccgag tcttgaatgg aagacgcttg
     6361 tgaggcgggc tgtgaggtcg ttgaaacaag gtggggggca tggtgggcgg caagaaccca
     6421 aggtcttgag gccttcgcta atgcgggaaa gctcttattc gggtgagatg ggctggggca
     6481 ccatctgggg accctgacgt gaagtttgtc actgactgga gaactcggtt tgtcgtctgt
     6541 tgcgggggcg gcagttatgg cggtgccgtt gggcagtgca cccgtacctt tgggagcgcg
     6601 cgccctcgtc gtgtcgtgac gtcacccgtt ctgttggctt ataatgcagg gtggggccac
     6661 ctgccggtag gtgtgcggta ggcttttctc cgtcgcagga cgcagggttc gggcctaggg
     6721 taggctctcc tgaatcgaca ggcgccggac ctctggtgag gggagggata agtgaggcgt
     6781 cagtttcttt ggtcggtttt atgtacctat cttcttaagt agctgaagct ccggttttga
     6841 actatgcgct cggggttggc gagtgtgttt tgtgaagttt tttaggcacc ttttgaaatg
     6901 taatcatttg ggtcaatatg taattttcag tgttagacta gtaaattgtc cgctaaattc
     6961 tggccgtttt tggctttttt gttagacgaa gcttggtacc atgagcaaag gagaagaact
     7021 tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt
     7081 ttctgtccgt ggagagggtg aaggtgatgc tacaaacgga aaactcaccc ttaaatttat
     7141 ttgcactact ggaaaactac ctgttccgtg gccaacactt gtcactactc tgacctatgg
     7201 tgttcaatgc ttttcccgtt atccggatca catgaaacgg catgactttt tcaagagtgc
     7261 catgcccgaa ggttatgtac aggaacgcac tatatctttc aaagatgacg ggacctacaa
     7321 gacgcgtgct gaagtcaagt ttgaaggtga tacccttgtt aatcgtatcg agttaaaggg
     7381 tattgatttt aaagaagatg gaaacattct tggacacaaa ctcgagtaca actttaactc
     7441 acacaatgta tacatcacgg cagacaaaca aaagaatgga atcaaagcta acttcaaaat
     7501 tcgccacaac gttgaagatg gttccgttca actagcagac cattatcaac aaaatactcc
     7561 aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtcgacac aatctgtcct
     7621 ttcgaaagat cccaacgaaa agcgtgacca catggtcctt cttgagtttg taactgctgc
     7681 tgggattaca catggcatgg atgagctcta caaataagcg gccgcgactc tagatcataa
     7741 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     7801 tgaacctgaa acataaaatg aatgcaattg ttgttgttta gtccctccca attcgatatc
     7861 aagcttatcg cgatagatcc taatcaacct ctggattaca aaatttgtga aagattgact
     7921 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     7981 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     8041 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     8101 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     8161 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     8221 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     8281 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     8341 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     8401 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     8461 gcctccccgc ctgagatcct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     8521 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     8581 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     8641 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     8701 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     8761 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     8821 caaagaaatg aatatcagag agtgagaggc ccgggttaat taaggaaagg gctagatcat
     8881 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     8941 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     9001 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     9061 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     9121 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     9181 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     9241 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     9301 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     9361 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     9421 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     9481 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     9541 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     9601 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     9661 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     9721 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     9781 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     9841 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     9901 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     9961 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
    10021 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
    10081 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
    10141 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
    10201 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
    10261 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
    10321 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
    10381 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
    10441 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
    10501 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
    10561 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
    10621 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
    10681 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
    10741 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
    10801 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
    10861 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
    10921 cgttggccga ttcattaatg cagcaagctc atggctgact aatttttttt atttatgcag
    10981 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
    11041 gcctaggctt ttgcaaaaag ctccccgtgg cacgacaggt ttcccgactg gaaagcgggc
    11101 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
    11161 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
    11221 aacagctatg acatgattac gaatttcaca aataaagcat ttttttcact gcattctagt
    11281 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa
    11341 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg
    11401 tggt
//
