LOCUS       sequence-347662-       11404 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a nuclease dead MRE11 in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11404)
  AUTHORS   Hussmann JA, Ling J, Ravisankar P, Yan J, Cirincione A, Xu A, 
            Simpson D, Yang D, Bothmer A, Cotta-Ramusino C, Weissman JS, Adamson
            B
  TITLE     Mapping the genetic landscape of DNA double-strand break repair.
  JOURNAL   Cell. 2021 Oct 28;184(22):5653-5669.e25. doi: 
            10.1016/j.cell.2021.10.002. Epub 2021 Oct 20.
  PUBMED    34672952
REFERENCE   2  (bases 1 to 11404)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.10.002"; journalName: "Cell"; date: 
            "2021-10-28- 28"; volume: "184"; issue: "22"; pages: "5653-5669.e25"
COMMENT     Sequence Label: pJL057
FEATURES             Location/Qualifiers
     source          1..11404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    664..789
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1286..1519
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1704..1748
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2041..2158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2222..3400
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2453..3391
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3348..3368
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5559..5585
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        5671..6882
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     6719..6738
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(6896..6898,6899..7612)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             6896..7609
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(6926..6954)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     7549..7569
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     misc_feature    7777..8365
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7830..7850)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8248..8259)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8440..8673
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8792..8810)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8878..8982
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8983..9051,9052..9843)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9201..9220)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10014..10602
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10503..10522
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10756..10773
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11013..11034
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11049..11054,11055..11072,11073..11079)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11087..11103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11092..11114
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11111..11127
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11111..11127
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11177..11196
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(11388..11404,1..617)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tcccaaagaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
       61 gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc
      121 tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc
      181 agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag
      241 tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac
      301 ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg
      361 aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct
      421 gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
      481 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
      541 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
      601 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
      661 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
      721 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
      781 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
      841 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
      901 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
      961 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     1021 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     1081 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggccgctga
     1141 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     1201 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     1261 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     1321 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     1381 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     1441 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     1501 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     1561 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     1621 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     1681 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     1741 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     1801 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     1861 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     1921 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
     1981 gattagtgaa cggatctcga cggtatcgcc gaattcacaa atggcagtat tcatccacaa
     2041 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     2101 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
     2161 ggtttattac agggacagca gagatccagt ttggactagg atccccagtt tggttagtac
     2221 cgggcccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
     2281 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
     2341 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
     2401 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
     2461 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
     2521 tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg
     2581 ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg
     2641 gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc
     2701 tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg
     2761 gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc
     2821 cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg
     2881 agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc
     2941 tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc
     3001 agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag
     3061 gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc
     3121 gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga
     3181 ttagttctcg cgcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat
     3241 ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta
     3301 attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac
     3361 agtggttcaa agtttttttc ttccatttca ggtgtcgtga gttaaccatg agtactgcag
     3421 atgcacttga tgatgaaaac acatttaaaa tattagttgc aacagatatt catcttggat
     3481 ttatggagaa agatgcagtc agaggaaatg atacgtttgt aacactcgat gaaattttaa
     3541 gacttgccca ggaaaatgaa gtggatttta ttttgttagg tggtgatctt tttcatgaaa
     3601 ataagccctc aaggaaaaca ttacatacct gcctcgagtt attaagaaaa tattgtatgg
     3661 gtgatcggcc tgtccagttt gaaattctca gtgatcagtc agtcaacttt ggttttagta
     3721 agtttccatg ggtgaactat caagatggca acctcaacat ttcaattcca gtgtttagta
     3781 ttcatggcaa taatgacgat cccacagggg cagatgcact ttgtgccttg gacattttaa
     3841 gttgtgctgg atttgtaaat cactttggac gttcaatgtc tgtggagaag atagacatta
     3901 gtccggtttt gcttcaaaaa ggaagcacaa agattgcgct atatggttta ggatccattc
     3961 cagatgaaag gctctatcga atgtttgtca ataaaaaagt aacaatgttg agaccaaagg
     4021 aagatgagaa ctcttggttt aacttatttg tgattcatca gaacaggagt aaacatggaa
     4081 gtactaactt cattccagaa caatttttgg atgacttcat tgatcttgtt atctggggcc
     4141 atgaacatga gtgtaaaata gctccaacca aaaatgaaca acagctgttt tatatctcac
     4201 aacctggaag ctcagtggtt acttctcttt ccccaggaga agctgtaaag aaacatgttg
     4261 gtttgctgcg tattaaaggg aggaagatga atatgcataa aattcctctt cacacagtgc
     4321 ggcagttttt catggaggat attgttctag ctaatcatcc agacattttt aacccagata
     4381 atcctaaagt aacccaagcc atacaaagct tctgtttgga gaagattgaa gaaatgcttg
     4441 aaaatgctga acgggaacgt ctgggtaatt ctcaccagcc agagaagcct cttgtacgac
     4501 tgcgagtgga ctatagtgga ggttttgaac ctttcagtgt tcttcgcttt agccagaaat
     4561 ttgtggatcg ggtagctaat ccaaaagaca ttatccattt tttcaggcat agagaacaaa
     4621 aggaaaaaac aggagaagag atcaactttg ggaaacttat cacaaagcct tcagaaggaa
     4681 caactttaag ggtagaagat cttgtaaaac agtactttca aaccgcagag aagaatgtgc
     4741 agctctcact gctaacagaa agagggatgg gtgaagcagt acaagaattt gtggacaagg
     4801 aggagaaaga tgccattgag gaattagtga aataccagtt ggaaaaaaca cagcgatttc
     4861 ttaaagaacg tcacattgat gccctcgaag acaaaatcga tgaggaggta cgtcgtttca
     4921 gagaaaccag acaaaaaaat actaatgaag aagatgatga agtccgtgag gctatgacca
     4981 gggccagagc actcagatct cagtcagagg agtctgcttc tgcctttagt gctgatgacc
     5041 ttatgagtat agatttagca gaacagatgg ctaatgactc tgatgatagc atctcagcag
     5101 caaccaacaa aggaagaggc cgaggaagag gtcgaagagg tggaagaggg cagaattcag
     5161 catcgagagg agggtctcaa agaggaagag cagacactgg tctggagact tctacccgta
     5221 gcaggaactc aaagactgct gtgtcagcat ctagaaatat gtctattata gatgccttta
     5281 aatctacaag acagcagcct tcccgaaatg tcactactaa gaattattca gaggtgattg
     5341 aggtagatga atcagatgtg gaagaagaca tttttcctac cacttcaaag acagatcaaa
     5401 ggtggtccag cacatcatcc agcaaaatca tgtcccagag tcaagtatcg aaaggggttg
     5461 attttgaatc aagtgaggat gatgatgatg atccttttat gaacactagt tctttaagaa
     5521 gaaatagaag ataaaatcta gagggcccgc ggttcgaata cccatacgac gtcccagact
     5581 acgcttagta atgattaatt aaactagaaa ttctaccggg taggggaggc gcttttccca
     5641 aggcagtctg gagtaaccca cccaagatct ggcctccgcg ccgggttttg gcgcctcccg
     5701 cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga cgaagggcgc agcgagcgtc
     5761 ctgatccttc cgcccggacg ctcaggacag cggcccgctg ctcataagac tcggccttag
     5821 aaccccagta tcagcagaag gacattttag gacgggactt gggtgactct agggcactgg
     5881 ttttctttcc agagagcgga acaggcgagg aaaagtagtc ccttctcggc gattctgcgg
     5941 agggatctcc gtggggcggt gaacgccgat gattatataa ggacgcgccg ggtgtggcac
     6001 agctagttcc gtcgcagccg ggatttgggt cgcggttctt gtttgtggat cgctgtgatc
     6061 gtcacttggt gagtagcggg ctgctgggct ggccggggct ttcgtggccg ccgggccgct
     6121 cggtgggacg gaagcgtgtg gagagaccgc caagggctgt agtctgggtc cgcgagcaag
     6181 gttgccctga actgggggtt ggggggagcg cagcaaaatg gcggctgttc ccgagtcttg
     6241 aatggaagac gcttgtgagg cgggctgtga ggtcgttgaa acaaggtggg gggcatggtg
     6301 ggcggcaaga acccaaggtc ttgaggcctt cgctaatgcg ggaaagctct tattcgggtg
     6361 agatgggctg gggcaccatc tggggaccct gacgtgaagt ttgtcactga ctggagaact
     6421 cggtttgtcg tctgttgcgg gggcggcagt tatggcggtg ccgttgggca gtgcacccgt
     6481 acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac ccgttctgtt ggcttataat
     6541 gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt ttctccgtcg caggacgcag
     6601 ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc cggacctctg gtgaggggag
     6661 ggataagtga ggcgtcagtt tctttggtcg gttttatgta cctatcttct taagtagctg
     6721 aagctccggt tttgaactat gcgctcgggg ttggcgagtg tgttttgtga agttttttag
     6781 gcaccttttg aaatgtaatc atttgggtca atatgtaatt ttcagtgtta gactagtaaa
     6841 ttgtccgcta aattctggcc gtttttggct tttttgttag acgaagcttg gtaccatgag
     6901 caaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt
     6961 taatgggcac aaattttctg tccgtggaga gggtgaaggt gatgctacaa acggaaaact
     7021 cacccttaaa tttatttgca ctactggaaa actacctgtt ccgtggccaa cacttgtcac
     7081 tactctgacc tatggtgttc aatgcttttc ccgttatccg gatcacatga aacggcatga
     7141 ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa cgcactatat ctttcaaaga
     7201 tgacgggacc tacaagacgc gtgctgaagt caagtttgaa ggtgataccc ttgttaatcg
     7261 tatcgagtta aagggtattg attttaaaga agatggaaac attcttggac acaaactcga
     7321 gtacaacttt aactcacaca atgtatacat cacggcagac aaacaaaaga atggaatcaa
     7381 agctaacttc aaaattcgcc acaacgttga agatggttcc gttcaactag cagaccatta
     7441 tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc
     7501 gacacaatct gtcctttcga aagatcccaa cgaaaagcgt gaccacatgg tccttcttga
     7561 gtttgtaact gctgctggga ttacacatgg catggatgag ctctacaaat aagcggccgc
     7621 gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     7681 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gtttagtccc
     7741 tcccaattcg atatcaagct tatcgcgata gatcctaatc aacctctgga ttacaaaatt
     7801 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     7861 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     7921 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     7981 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     8041 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     8101 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     8161 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     8221 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     8281 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     8341 atctcccttt gggccgcctc cccgcctgag atcctttaag accaatgact tacaaggcag
     8401 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     8461 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     8521 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     8581 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     8641 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     8701 agtatttata acttgcaaag aaatgaatat cagagagtga gaggcccggg ttaattaagg
     8761 aaagggctag atcattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt
     8821 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     8881 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     8941 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     9001 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     9061 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     9121 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     9181 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa
     9241 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     9301 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     9361 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     9421 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     9481 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     9541 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     9601 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     9661 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     9721 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     9781 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     9841 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     9901 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     9961 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
    10021 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
    10081 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
    10141 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
    10201 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
    10261 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
    10321 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
    10381 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
    10441 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
    10501 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
    10561 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
    10621 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
    10681 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
    10741 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
    10801 ccgcctctcc ccgcgcgttg gccgattcat taatgcagca agctcatggc tgactaattt
    10861 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
    10921 gaggcttttt tggaggccta ggcttttgca aaaagctccc cgtggcacga caggtttccc
    10981 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
    11041 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
    11101 caatttcaca caggaaacag ctatgacatg attacgaatt tcacaaataa agcatttttt
    11161 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
    11221 aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc
    11281 actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga attattttca
    11341 ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagttgg aagggctaat
    11401 tcac
//
