LOCUS       sequence-347663-        9280 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bicistronic vector with GFP reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9280)
  AUTHORS   Hussmann JA, Ling J, Ravisankar P, Yan J, Cirincione A, Xu A, 
            Simpson D, Yang D, Bothmer A, Cotta-Ramusino C, Weissman JS, Adamson
            B
  TITLE     Mapping the genetic landscape of DNA double-strand break repair.
  JOURNAL   Cell. 2021 Oct 28;184(22):5653-5669.e25. doi: 
            10.1016/j.cell.2021.10.002. Epub 2021 Oct 20.
  PUBMED    34672952
REFERENCE   2  (bases 1 to 9280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.10.002"; journalName: "Cell"; date: 
            "2021-10-28- 28"; volume: "184"; issue: "22"; pages: "5653-5669.e25"
COMMENT     Sequence Label: pJL059
FEATURES             Location/Qualifiers
     source          1..9280
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             124..757
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    804..929
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1426..1659
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1844..1888
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2181..2298
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2362..3540
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2593..3531
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3488..3508
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3575..3601
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        3687..4898
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4735..4754
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(4912..4914,4915..5628)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             4912..5625
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(4942..4970)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     5565..5585
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     misc_feature    5793..6381
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5846..5866)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6264..6275)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6456..6689
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6808..6826)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6894..6998
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6999..7067,7068..7859)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7217..7236)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8030..8618
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8519..8538
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8772..8789
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9029..9050
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9065..9070,9071..9088,9089..9095)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9103..9119
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9108..9130
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9127..9143
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9127..9143
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9193..9212
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc
       61 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt
      121 agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc
      181 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc
      241 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg
      301 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc
      361 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc
      421 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt
      481 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca
      541 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc
      601 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      661 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      721 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      781 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      841 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      901 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      961 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     1021 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     1081 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     1141 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     1201 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     1261 agcaagcggc cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     1321 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     1381 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     1441 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     1501 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     1561 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     1621 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     1681 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     1741 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     1801 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     1861 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     1921 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     1981 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     2041 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     2101 agacagagac agatccattc gattagtgaa cggatctcga cggtatcgcc gaattcacaa
     2161 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     2221 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     2281 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt ttggactagg
     2341 atccccagtt tggttagtac cgggcccggt gcccgtcagt gggcagagcg cacatcgccc
     2401 acagtccccg agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg
     2461 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
     2521 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
     2581 gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat
     2641 ggcccttgcg tgccttgaat tacttccacc tggctgcagt acgtgattct tgatcccgag
     2701 cttcgggttg gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct
     2761 cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac
     2821 cttcgcgcct gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct
     2881 gctgcgacgc tttttttctg gcaagatagt cttgtaaatg cgggccaaga tctgcacact
     2941 ggtatttcgg tttttggggc cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt
     3001 tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg gacgggggta gtctcaagct
     3061 ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca
     3121 aggctggccc ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc cggccctgct
     3181 gcagggagct caaaatggag gacgcggcgc tcgggagagc gggcgggtga gtcacccaca
     3241 caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg
     3301 gcgccgtcca ggcacctcga ttagttctcg cgcttttgga gtacgtcgtc tttaggttgg
     3361 ggggaggggt tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg
     3421 ccagcttggc acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg
     3481 ttcattctca agcctcagac agtggttcaa agtttttttc ttccatttca ggtgtcgtga
     3541 gttaacctcg agtctagagg gcccgcggtt cgaataccca tacgacgtcc cagactacgc
     3601 ttagtaatga ttaattaaac tagaaattct accgggtagg ggaggcgctt ttcccaaggc
     3661 agtctggagt aacccaccca agatctggcc tccgcgccgg gttttggcgc ctcccgcggg
     3721 cgcccccctc ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga
     3781 tccttccgcc cggacgctca ggacagcggc ccgctgctca taagactcgg ccttagaacc
     3841 ccagtatcag cagaaggaca ttttaggacg ggacttgggt gactctaggg cactggtttt
     3901 ctttccagag agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg
     3961 atctccgtgg ggcggtgaac gccgatgatt atataaggac gcgccgggtg tggcacagct
     4021 agttccgtcg cagccgggat ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca
     4081 cttggtgagt agcgggctgc tgggctggcc ggggctttcg tggccgccgg gccgctcggt
     4141 gggacggaag cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg agcaaggttg
     4201 ccctgaactg ggggttgggg ggagcgcagc aaaatggcgg ctgttcccga gtcttgaatg
     4261 gaagacgctt gtgaggcggg ctgtgaggtc gttgaaacaa ggtggggggc atggtgggcg
     4321 gcaagaaccc aaggtcttga ggccttcgct aatgcgggaa agctcttatt cgggtgagat
     4381 gggctggggc accatctggg gaccctgacg tgaagtttgt cactgactgg agaactcggt
     4441 ttgtcgtctg ttgcgggggc ggcagttatg gcggtgccgt tgggcagtgc acccgtacct
     4501 ttgggagcgc gcgccctcgt cgtgtcgtga cgtcacccgt tctgttggct tataatgcag
     4561 ggtggggcca cctgccggta ggtgtgcggt aggcttttct ccgtcgcagg acgcagggtt
     4621 cgggcctagg gtaggctctc ctgaatcgac aggcgccgga cctctggtga ggggagggat
     4681 aagtgaggcg tcagtttctt tggtcggttt tatgtaccta tcttcttaag tagctgaagc
     4741 tccggttttg aactatgcgc tcggggttgg cgagtgtgtt ttgtgaagtt ttttaggcac
     4801 cttttgaaat gtaatcattt gggtcaatat gtaattttca gtgttagact agtaaattgt
     4861 ccgctaaatt ctggccgttt ttggcttttt tgttagacga agcttggtac catgagcaaa
     4921 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat
     4981 gggcacaaat tttctgtccg tggagagggt gaaggtgatg ctacaaacgg aaaactcacc
     5041 cttaaattta tttgcactac tggaaaacta cctgttccgt ggccaacact tgtcactact
     5101 ctgacctatg gtgttcaatg cttttcccgt tatccggatc acatgaaacg gcatgacttt
     5161 ttcaagagtg ccatgcccga aggttatgta caggaacgca ctatatcttt caaagatgac
     5221 gggacctaca agacgcgtgc tgaagtcaag tttgaaggtg atacccttgt taatcgtatc
     5281 gagttaaagg gtattgattt taaagaagat ggaaacattc ttggacacaa actcgagtac
     5341 aactttaact cacacaatgt atacatcacg gcagacaaac aaaagaatgg aatcaaagct
     5401 aacttcaaaa ttcgccacaa cgttgaagat ggttccgttc aactagcaga ccattatcaa
     5461 caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta cctgtcgaca
     5521 caatctgtcc tttcgaaaga tcccaacgaa aagcgtgacc acatggtcct tcttgagttt
     5581 gtaactgctg ctgggattac acatggcatg gatgagctct acaaataagc ggccgcgact
     5641 ctagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     5701 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgttt agtccctccc
     5761 aattcgatat caagcttatc gcgatagatc ctaatcaacc tctggattac aaaatttgtg
     5821 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     5881 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     5941 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     6001 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     6061 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     6121 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     6181 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     6241 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     6301 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     6361 ccctttgggc cgcctccccg cctgagatcc tttaagacca atgacttaca aggcagctgt
     6421 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     6481 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     6541 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     6601 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     6661 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     6721 tttataactt gcaaagaaat gaatatcaga gagtgagagg cccgggttaa ttaaggaaag
     6781 ggctagatca ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     6841 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     6901 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     6961 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     7021 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     7081 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     7141 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     7201 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc
     7261 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     7321 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     7381 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     7441 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     7501 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     7561 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     7621 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     7681 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     7741 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     7801 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     7861 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     7921 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     7981 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     8041 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     8101 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     8161 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     8221 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     8281 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     8341 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     8401 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     8461 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     8521 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     8581 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     8641 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     8701 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     8761 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     8821 ctctccccgc gcgttggccg attcattaat gcagcaagct catggctgac taattttttt
     8881 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     8941 cttttttgga ggcctaggct tttgcaaaaa gctccccgtg gcacgacagg tttcccgact
     9001 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     9061 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     9121 ttcacacagg aaacagctat gacatgatta cgaatttcac aaataaagca tttttttcac
     9181 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact
     9241 ggataactca agctaaccaa aatcatccca aacttcccac
//
