LOCUS       sequence-347669-        9895 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9895)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3151
FEATURES             Location/Qualifiers
     source          1..9895
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2649..2673)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2681..2722
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2833..3421
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2886..2906)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3304..3315)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3493..3726
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3804..3925
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3841..3860)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3895..3914
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3965..4100
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4120..4139)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4121..4139)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4149..4166)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4149..4165)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4158..4180)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4307..4762
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4394..4413)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4604..4625
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4788..4892
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4893..4961,4962..5753)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5111..5130)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5924..6512
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6413..6432
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6666..6683
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6800..6821
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6836..6841,6842..6859,6860..6866)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6874..6890
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6879..6901
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6898..6914
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6898..6914
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6933..6953
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6935..6953
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6981..7207
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7208..7388
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7435..7560
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8053..8286
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8471..8515
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8746..8956
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8768..8964
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8815..8950
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8996..9598
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8996..9015)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9492..9512
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9641..9758
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcaccatgac cacattcaag gaggcagtga ccttcaagga
     1261 tgtggctgtg ttcttcactg aggaggaact ggggctactg gaccctgccc agaggaagct
     1321 gtaccaagac gtgatgcttg agaacttcac gaacctgctg tcagtggggc atcaaccatt
     1381 ccaccctttc cacttcctaa gggaagaaaa gttttggatg atggagacag caacccaaag
     1441 agaaggaaat tcaggcggca agactattgc ggaagcagga ccacatgaag actgcccttg
     1501 ccagcaaatc tgggaacaaa ctgcaagtga cttaacccag tctcaagact ccatcataaa
     1561 taattctcac ttctttgaac aaggtgatgt cccctcccag gttgaggcag gactatctat
     1621 aattcataca ggacagaaac cttcacagaa tgggaagtgt aaacagtcct tcagtgatgt
     1681 tgccatcttt gatcctcctc agcagttcca ctcaggagag aagtctcata catgcaatga
     1741 gtgtggaaaa agcttctgtt acatctcagc tcttcgtatt caccagagag ttcacttgag
     1801 agagaaactc tctaagtgtg acatgcgtgg taaggaattc agtcagagct catgtctgca
     1861 aactcgtgag agagtccaca ctggagagaa accattcaaa tgtgagcaat gtgggaaagg
     1921 cttcagatgt agagcgatac ttcaagttca ctgcaaatta cacacaggag agaaacctta
     1981 tatttgtgag aaatgtggga gggccttcat tcacgatttc cagcttcaga aacatcagat
     2041 aattcatact ggggagaagc cgttcaaatg tgaaatatgt ggtaagagct tctgccttag
     2101 gtcaagtctt aataggcatt gcatggtcca cacagcagag aaactgtaca aatctgagga
     2161 gtgtggaaaa ggcttcactg atagcctaga tttgcataag catcagataa ttcacacagg
     2221 acagaaaccg tacaattgta aagaatgtgg gaagagcttc agatggtcct catatctttt
     2281 gatccatcag cgaatccaca gtggagaaaa accatacaga tgtgaggagt gtgggaaggg
     2341 ctacattagt aagtcaggtc ttaacttgca ccagagggtc catactggag agagacctta
     2401 taattgtaag gaatgtggga agagctttag ccgggcttca agtattttga atcataagaa
     2461 actccactgc cggaaaaaac ccttcaaatg tgaggattgt ggaaagaggc ttgtacaccg
     2521 gtctttctgt aaagaccaac aaggagacca caatggagaa aactcatcca aatgtgagga
     2581 ctgtgggaag cgctacaaga ggcgcttgaa tctggatata attttatcat tatttttaaa
     2641 tgatatgttg ccaactttct tgtacaaagt ggttgatatc ggtaagccta tccctaaccc
     2701 tctcctcggt ctcgattcta cgtagtaatg aactagtccg taacttgaaa gtatttcgat
     2761 ttcttggctt tatatatctt gtggaaagga cgactgcgaa gacggcaaca tcagataacg
     2821 cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     2881 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     2941 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     3001 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     3061 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     3121 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     3181 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     3241 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     3301 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     3361 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     3421 cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     3481 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     3541 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     3601 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     3661 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     3721 ctagcagtac gtatagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     3781 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     3841 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     3901 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     3961 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     4021 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     4081 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
     4141 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     4201 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     4261 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     4321 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     4381 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     4441 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     4501 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     4561 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     4621 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     4681 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     4741 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     4801 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     4861 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     4921 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     4981 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     5041 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     5101 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     5161 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     5221 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     5281 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     5341 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     5401 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     5461 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     5521 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     5581 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     5641 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     5701 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     5761 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     5821 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     5881 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     5941 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     6001 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     6061 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     6121 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     6181 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     6241 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     6301 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     6361 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     6421 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     6481 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     6541 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     6601 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     6661 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     6721 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     6781 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     6841 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     6901 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
     6961 agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt
     7021 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt
     7081 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat
     7141 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata
     7201 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     7261 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     7321 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     7381 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     7441 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     7501 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     7561 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     7621 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     7681 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     7741 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     7801 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     7861 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     7921 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     7981 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     8041 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     8101 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     8161 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     8221 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     8281 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     8341 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     8401 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     8461 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     8521 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     8581 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     8641 atattcacca ttatcgtttc agacccacct cccaaccccg aggggaccca tgcattgcat
     8701 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     8761 caaagcatgc gtctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     8821 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     8881 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     8941 tttggaggcc taggcttttg caaaaagctt tctagaggta ccaccggagc ttaccatgac
     9001 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     9061 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     9121 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     9181 caaggtgtgg gtcgcggacg acggcgccgc cgtggcggtc tggaccacgc cggagagcgt
     9241 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     9301 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     9361 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     9421 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     9481 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     9541 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg ccagttaacg
     9601 aattctagat cttgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     9661 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     9721 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     9781 gagatccact ttggcgccgg ctcgaggcct gcaggtgcaa agatggataa agttttaaac
     9841 agagaggaat ctttgcagct aatggacctt ctaggtcttg aaaggagtgg gaatt
//
