LOCUS       sequence-347673-        9856 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9856)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3154
FEATURES             Location/Qualifiers
     source          1..9856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2610..2634)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2642..2683
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2794..3382
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2847..2867)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3265..3276)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3454..3687
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3765..3886
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3802..3821)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3856..3875
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3926..4061
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4081..4100)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4082..4100)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4110..4127)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4110..4126)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4119..4141)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4268..4723
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4355..4374)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4565..4586
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4749..4853
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4854..4922,4923..5714)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5072..5091)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5885..6473
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6374..6393
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6627..6644
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6761..6782
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6797..6802,6803..6820,6821..6827)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6835..6851
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6840..6862
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6859..6875
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6859..6875
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6894..6914
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6896..6914
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6942..7168
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7169..7349
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7396..7521
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8014..8247
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8432..8476
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8707..8917
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8729..8925
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8776..8911
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8957..9559
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8957..8976)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9453..9473
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9602..9719
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcaccatgga aagggaaggt atctggcatt ctactctagg
     1261 ggaaacctgg gaacctaata attggttaga gggacaacag gatagtcatc tgagccaagt
     1321 gggagttacc cataaggaaa ccttcactga gatgagagta tgtggaggta atgaatttga
     1381 aagatgttcc agtcaggatt caatccttga tacacagcaa agcattccta tggtaaaaag
     1441 gccccataac tgtaattcac atggagaaga tgccacacaa aattctgagt taattaaaac
     1501 tcaaagaatg tttgtaggaa agaagatcta tgaatgtaat cagtgcagca aaaccttcag
     1561 tcagagctca tcccttctta agcaccagag gattcatact ggggagaaac cctataagtg
     1621 taatgtatgt gggaaacact tcattgaacg atcctccctt actgtacatc aaagaattca
     1681 tactggagag aaaccctata aatgtaatga atgtgggaaa gcctttagtc agagcatgaa
     1741 tcttactgtc catcaacgaa ctcacaccgg agagaaaccc tatcagtgta aagagtgtgg
     1801 caaagccttc cataagaatt catctcttat tcagcatgaa aggattcata ctggagagaa
     1861 accctacaaa tgtaatgaat gtggtaaagc ttttacccaa agcatgaatt tgacagttca
     1921 tcagagaact catacaggag aaaaacccta tgaatgtaat gaatgtggaa aagccttcag
     1981 tcaaagcatg catcttattg tacatcagag aagccatact ggagaaaaac cctatgagtg
     2041 tagtcagtgt ggaaaagcct ttagtaagag ctcaactctt accctacatc agcgaaatca
     2101 cactggagaa aaaccttaca aatgtaacaa atgcgggaaa tcctttagcc aaagtacata
     2161 tcttatagaa catcagagac ttcattctgg agtaaaacct tttgaatgta acgagtgtgg
     2221 aaaagctttc agtaagaatt catctctaac tcaacatcgg agaattcaca ctggagagaa
     2281 accttatgag tgtatggtgt gtggaaaaca tttcactgga cgatcatccc ttaccgtgca
     2341 tcaggtcatt cacactggag agaaacctta tgagtgcaat gaatgtggaa aggcattcag
     2401 ccagagtgct taccttattg aacatcaaag aattcatact ggtgagaaac cctatgaatg
     2461 tgatcagtgt ggaaaagcct tcattaagaa ttcatccctt acagtgcatc agagaactca
     2521 tacaggagag aaaccctatc agtgtaatga atgcggaaaa gccttcagcc ggagtacaaa
     2581 ccttacacga catcaaagaa ctcatacgtt gccaactttc ttgtacaaag tggttgatat
     2641 cggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat gaactagccc
     2701 gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acgaccaaat
     2761 cacgatattt tgagtataac gcgttaagtc gacaatcaac ctctggatta caaaatttgt
     2821 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     2881 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     2941 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     3001 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     3061 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     3121 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     3181 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     3241 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     3301 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     3361 tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag
     3421 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     3481 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     3541 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     3601 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     3661 tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc
     3721 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     3781 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     3841 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     3901 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     3961 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     4021 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     4081 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     4141 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     4201 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     4261 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     4321 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     4381 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     4441 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     4501 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     4561 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     4621 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     4681 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     4741 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     4801 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     4861 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     4921 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     4981 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     5041 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     5101 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     5161 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     5221 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     5281 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     5341 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     5401 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     5461 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     5521 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     5581 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     5641 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     5701 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     5761 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     5821 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     5881 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     5941 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     6001 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     6061 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     6121 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     6181 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     6241 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     6301 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     6361 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     6421 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     6481 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     6541 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     6601 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     6661 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     6721 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     6781 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     6841 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     6901 accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact
     6961 cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa
     7021 gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca
     7081 acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt
     7141 atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg
     7201 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     7261 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     7321 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     7381 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     7441 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     7501 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     7561 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     7621 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     7681 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     7741 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     7801 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     7861 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     7921 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     7981 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     8041 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     8101 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     8161 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     8221 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     8281 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     8341 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     8401 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     8461 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     8521 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     8581 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     8641 gaggggaccc atgcattgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     8701 tccccagcag gcagaagtat gcaaagcatg cgtctcaatt agtcagcaac catagtcccg
     8761 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     8821 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     8881 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ttctagaggt
     8941 accaccggag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     9001 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     9061 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     9121 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg ccgtggcggt
     9181 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     9241 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     9301 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     9361 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     9421 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     9481 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     9541 caagcccggt gccagttaac gaattctaga tcttgagaca aatggcagta ttcatccaca
     9601 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     9661 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
     9721 gggtttatta cagggacagc agagatccac tttggcgccg gctcgaggcc tgcaggtgca
     9781 aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct tctaggtctt
     9841 gaaaggagtg ggaatt
//
