LOCUS       sequence-34771-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  TITLE     Cookson Lab Plasmids
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDEST40-DJ1-E18N
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..18)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(28..69)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    95..119
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(717..741)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(770..789)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(771..789)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(834..878)
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
ORIGIN
        1 atggtgatgg tgatgatgac cggtacgcgt agaatcgaga ccgaggagag ggttagggat
       61 aggcttacct tcgaaccgcg ggccctctag atcaaccact ttgtacaaga aagctgggtc
      121 gaattcgccc ttcttgtctt taagaacaag tggagccttc acttgagccg ccacctcctt
      181 gccattcagg gcttcaacaa ttgcaagcgc aaactcgaag ctggtcccag gcccccggct
      241 tgtaagaatc aggccgtctt tttccacacg attctcagag taggtgtaat gacctccatt
      301 catcattttg tctttagcaa gagggtgtgt tgtaacttta cttccaaaac ctatttcatg
      361 agccaacaga gcagtaggac ctgcacagat ggcggctatc aggcccttcc ggttttcctg
      421 ctccttcagt atctccttca cagcagcaga ctcagataaa ttctgtgcgc ccagattacc
      481 tcctggtaga accaccacat catatggtcc ctcttttttt gcatcttcaa ggctggcatc
      541 aggacaaatg accacatcac ggctacactg tactgggtct tttccagcca ggcctgcaac
      601 ggtgacctta atcccagctc gcctcatgac atctacaggg atgaccgtgt tcatttcctc
      661 tgctccttta gccaggatga ccagagctct tttggaagcc ataagggcga attcggagcc
      721 tgcttttttg tacaaacttg ttgatagctt aactagccag cttgggtctc cctatagtga
      781 gtcgtattaa tttcgataag ccagtaagca gtgggttctc tagttagcca gagagctctg
      841 cttatataga cctcccaccg tacacgccta ccgcccat
//
