LOCUS       sequence-34772-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  TITLE     Cookson Lab Plasmids
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDEST40-DJ1-C106A
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(4..21)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(31..72)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    98..122
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(720..744)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(773..792)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(774..792)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctcatggtga tggtgatgat gaccggtacg cgtagaatcg agaccgagga gagggttagg
       61 gataggctta ccttcgaacc gcgggccctc tagatcaacc actttgtaca agaaagctgg
      121 gtcgaattcg cccttcttgt ctttaagaac aagtggagcc ttcacttgag ccgccacctc
      181 cttgccattc agggcttcaa caattgcaag cgcaaactcg aagctggtcc caggcccccg
      241 gcttgtaaga atcaggccgt ctttttccac acgattctca gagtaggtgt aatgacctcc
      301 attcatcatt ttgtctttag caagagggtg tgttgtaact ttacttccaa aacctatttc
      361 atgagccaac agagcagtag gacctgcagc gatggcggct atcaggccct tccggttttc
      421 ctgctccttc agtatctcct tcacagcagc agactcagat aaattctgtg cgcccagatt
      481 acctcctggt agaaccacca catcatatgg tccctctttt tttgcatctt caaggctggc
      541 atcaggacaa atgaccacat cacggctaca ctgtactggg tcttttccag ccaggcctgc
      601 aacggtgacc ttaatcccag ctcgcctcat gacatctaca gggatgaccg tctccatttc
      661 ctctgctcct ttagccagga tgaccagagc tcttttggaa gccataaggg cgaattcgga
      721 gcctgctttt ttgtacaaac ttgttgatag cttaactagc cagcttgggt ctccctatag
      781 tgagtcgtat taatttcgat aagccagtaa gcagtgggtt ctctagttag ccagagagct
      841 ctgcttatat agacctccca ccgtaca
//
