LOCUS       sequence-34775-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  TITLE     Cookson Lab Plasmids
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti6-DJ1-V5-E18N
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(10..51)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    104..128
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(726..750)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 accggtacgc gtagaatcga gaccgaggag agggttaggg ataggcttac cttcgaaccg
       61 cgggccctct agactcgagc ggccgccact gtgctggata tcaaccactt tgtacaagaa
      121 agctgggtcg aattcgccct tcttgtcttt aagaacaagt ggagccttca cttgagccgc
      181 cacctccttg ccattcaggg cttcaacaat tgcaagcgca aactcgaagc tggtcccagg
      241 cccccggctt gtaagaatca ggccgtcttt ttccacacga ttctcagagt aggtgtaatg
      301 acctccattc atcattttgt ctttagcaag agggtgtgtt gtaactttac ttccaaaacc
      361 tatttcatga gccaacagag cagtaggacc tgcacagatg gcggctatca ggcccttccg
      421 gttttcctgc tccttcagta tctccttcac agcagcagac tcagataaat tctgtgcgcc
      481 cagattacct cctggtagaa ccaccacatc atatggtccc tctttttttg catcttcaag
      541 gctggcatca ggacaaatga ccacatcacg gctacactgt actgggtctt ttccagccag
      601 gcctgcaacg gtgaccttaa tcccagctcg cctcatgaca tctacaggga tgaccgtgtt
      661 catttcctct gctcctttag ccaggatgac cagagctctt ttggaagcca taagggcgaa
      721 ttcggagcct gcttttttgt acaaacttgt tgatatctgc agaattccac cacactggac
      781 tagtggatcc tctagagtcg gtgtcttcta tggaggtcaa aacagcgtgg atggcgtctc
      841 caggcgatct gacggttcac taaacga
//
