LOCUS       sequence-347838-        9009 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TetO-Lmx1a-t2a-Pitx3 in a PiggyBac vector with puromycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9009)
  AUTHORS   Ng YH, Chanda S, Janas JA, Yang N, Kokubu Y, Sudhof TC, Wernig M
  TITLE     Efficient generation of dopaminergic induced neuronal cells with 
            midbrain characteristics.
  JOURNAL   Stem Cell Reports. 2021 Jul 13;16(7):1763-1776. doi: 
            10.1016/j.stemcr.2021.05.017. Epub 2021 Jun 24.
  PUBMED    34171286
REFERENCE   2  (bases 1 to 9009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2021.05.017"; journalName: "Stem Cell Reports"; 
            date: "2021-07-13- 13"; volume: "16"; issue: "7"; pages: "1763-1776"
COMMENT     Sequence Label: PiggyBac-TetO-Lmx1a-t2a-Pitx3-puro
FEATURES             Location/Qualifiers
     source          1..9009
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(52..69)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(223..811)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(303..322)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(982..1773,1774..1842))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1605..1624
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1843..1947)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1973..2428)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2110..2131)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2322..2341
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2555..2577
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2569..2586
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2570..2586
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   2716..2778
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    join(3220..3238,3239..3261,3262..3280,3281..3303,3304..3322,
                     3323..3345,3346..3364,3365..3387,3388..3406,3407..3429,
                     3430..3448,3449..3471,3472..3490)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        3523..3560
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3557..3581
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4850..4903
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(5863..5880)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5869..6093
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        6189..6400
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             6413..6681
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             6708..7307
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6708..6727)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7204..7224
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(7367..7387)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    7422..7543
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7459..7478)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7513..7532
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   8305..8339
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8830..8846)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8830..8846)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8843..8865)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8854..8870
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8878..8884,8885..8902,8903..8908))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8923..8944
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
       61 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
      121 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
      181 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
      241 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
      301 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
      361 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
      421 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
      481 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
      541 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
      601 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
      661 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
      721 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
      781 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
      841 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
      901 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
      961 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     1021 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     1081 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     1141 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     1201 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     1261 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     1321 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     1381 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     1441 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     1501 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     1561 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     1621 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     1681 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     1741 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     1801 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     1861 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     1921 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta
     1981 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     2041 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     2101 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     2161 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     2221 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     2281 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     2341 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     2401 gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg
     2461 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     2521 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     2581 gccagtgagc gcgcctcgtt cattcacgtt tttgaacccg tggaggacgg gcagactcgc
     2641 ggtgcaaatg tgttttacag cgtgatggag cagatgaaga tgctcgacac gctgcagaac
     2701 acgcagctag attaacccta gaaagataat catattgtga cgtacgttaa agataatcat
     2761 gcgtaaaatt gacgcatgtg ttttatcggt ctgtatatcg aggtttattt attaatttga
     2821 atagatatta agttttatta tatttacact tacatactaa taataaattc aacaaacaat
     2881 ttatttatgt ttatttattt attaaaaaaa aacaaaaact caaaatttct tctataaagt
     2941 aacaaaactt ttatgaggga cagccccccc ccaaagcccc cagggatgta attacgtccc
     3001 tcccccgcta gggggcagca gcgagccgcc cggggctccg ctccggtccg gcgctccccc
     3061 cgcatccccg agccggcagc gtgcggggac agcccgggca cggggaaggt ggcacgggat
     3121 cgctttcctc tgaacgcttc tcgctgctct ttgagcctgc agacacctgg ggggatacgg
     3181 ggaaaaggcc tccaaggcca ctagtctcga gtttaccact ccctatcagt gatagagaaa
     3241 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
     3301 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
     3361 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
     3421 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     3481 gtgatagaga aaagtgaaag tcgagctcgg tacccgggtc gagtaggcgt gtacggtggg
     3541 aggcctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac
     3601 gctgttttga cctccataga agacaccggg accgatccag cctccgcggc cccgaattcg
     3661 aagactagga tccgccacca tgctggacgg cctaaagatg gaggagaact tccaaagcgc
     3721 gatcgacacc tcggcctcct tctcctcgct gctgggcaga gcggtgagcc ccaagtctgt
     3781 ctgcgagggc tgtcagcggg tcatcttgga caggtttctg ctgcggctca acgacagctt
     3841 ctggcatgag cagtgcgtgc agtgcgcctc ctgcaaagag cccctggaga ccacctgctt
     3901 ctaccgggac aagaagctgt actgcaagta tgactacgag aagctgtttg ctgttaaatg
     3961 tgggggctgc ttcgaggcca tcgctcccaa tgagtttgtt atgcgggccc agaagagtgt
     4021 ataccacctg agctgcttct gctgctgtgt ctgcgagcga cagcttcaga agggtgatga
     4081 gtttgtcctg aaggaggggc agctgctctg caaaggggac tatgagaagg agcgggagct
     4141 gctcagcctg gtgagcccag cagcctcaga ctcaggtaaa agtgatgatg aagaaagtct
     4201 ctgcaagtca gcccatgggg cagggaaagg aactgctgag gaaggcaagg accataagcg
     4261 ccccaaacgt ccgagaacca tcttgacaac tcaacagagg cgagcattca aggcctcatt
     4321 tgaagtatcc tccaagccct gcaggaaggt gagagagact ctggctgcag agacagggct
     4381 gagtgtccgt gtcgtccagg tgtggttcca aaaccagaga gcgaagatga agaagctggc
     4441 caggcgacag cagcagcagc agcaagatca gcagaacacc cagaggctga gctctgctca
     4501 gacaaacggt ggtgggagtg ctgggatgga aggaatcatg aacccctaca cggctctgcc
     4561 caccccacag cagctcctgg ccatcgagca gagtgtctac agctcagatc ccttccgaca
     4621 gggtctcacc ccaccccaga tgcctggaga ccacatgcac ccttatggtg ccgagcccct
     4681 tttccatgac ctggatagcg acgacacctc cctcagtaac ctgggtgact gtttcctagc
     4741 aacctcagaa gctgggcctc tgcagtccag agtgggaaac cccattgacc atctgtactc
     4801 catgcagaat tcttacttca catctagatc catggctagc ggctccggcg agggcagggg
     4861 aagtcttcta acatgcgggg acgtggagga aaatcccggc ccagcatgct ctagaatgga
     4921 gttcggcctg ctcagcgagg cagaggcccg gagccctgcc ctgtcgctgt cagacgctgg
     4981 cactccgcac ccccagctcc cagagcacgg ctgcaagggc caggagcaca gcgactcaga
     5041 aaaggcctcg gcttcgctgc ccggcggctc cccagaggac ggttcgctga aaaagaagca
     5101 gcggcggcag cgcacgcact tcaccagcca gcagctacag gagctagagg cgaccttcca
     5161 gaggaaccgc taccccgaca tgagcacgcg cgaggagatc gccgtgtgga ccaacctcac
     5221 cgaggcccgc gtgcgggtgt ggttcaagaa ccggcgcgcc aaatggcgga agcgcgagcg
     5281 cagccagcag gccgagctat gcaaaggcag cttcgcggcg ccgctcgggg ggctggtgcc
     5341 gccctacgag gaggtgtacc ccggctactc gtacggcaac tggccgccca aggctcttgc
     5401 cccgccgctc gccgccaaga cctttccatt cgccttcaac tcggtcaacg tggggcctct
     5461 ggcttcgcag cccgtcttct cgccacccag ctccatcgcc gcctccatgg tgccctccgc
     5521 cgcggctgcc ccgggcaccg tgccagggcc tggggccctg cagggcctgg gcgggggccc
     5581 ccccgggctg gctccggccg ccgtgtcctc cggggccgtg tcctgccctt atgcctcggc
     5641 cgccgccgcc gccgcggctg ccgcctcttc cccctacgtc tatcgggacc cgtgtaactc
     5701 gagcctggcc agcctgcggc tcaaagccaa acagcacgcc tccttcagct accccgctgt
     5761 gcacgggccg cccccggcag ccaaccttag tccgtgccag tacgccgtgg aaaggcccgt
     5821 atgattaatt aagctagccc taggctagag ctcgctgatc agcctcgact gtgccttcta
     5881 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     5941 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     6001 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     6061 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     6121 gctcgactag agcttgcgga acccttcgcg gccgcgaagg atctgcgatc gctccggtgc
     6181 ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg
     6241 caattgaacg ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta
     6301 ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt
     6361 gaacgttctt tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg
     6421 catctctcct tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg
     6481 cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt
     6541 aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag
     6601 ccggctctcc acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc
     6661 tgttctgcgc cgttacagat ccaagctgtg accggcgcct acgctagatg accgagtaca
     6721 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     6781 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     6841 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     6901 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     6961 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     7021 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     7081 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     7141 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     7201 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     7261 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgagtc gacaatcaac
     7321 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     7381 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgt taacttgttt attgcagctt
     7441 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     7501 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggaattgac
     7561 tcaaatgatg tcaattagtc tatcagaagc tatctggtct cccttccggg ggacaagaca
     7621 tccctgttta atatttaaac agcagtgttc ccaaactggg ttcttatatc ccttgctctg
     7681 gtcaaccagg ttgcagggtt tcctgtcctc acaggaacga agtccctaaa gaaacagtgg
     7741 cagccaggtt tagccccgga attgactgga ttcctttttt agggcccatt ggtatggctt
     7801 tttccccgta tccccccagg tgtctgcagg ctcaaagagc agcgagaagc gttcagagga
     7861 aagcgatccc gtgccacctt ccccgtgccc gggctgtccc cgcacgctgc cggctcgggg
     7921 atgcgggggg agcgccggac cggagcggag ccccgggcgg ctcgctgctg ccccctagcg
     7981 ggggagggac gtaattacat ccctgggggc tttggggggg ggctgtccct gatatctata
     8041 acaagaaaat atatatataa taagttatca cgtaagtaga acatgaaata acaatataat
     8101 tatcgtatga gttaaatctt aaaagtcacg taaaagataa tcatgcgtca ttttgactca
     8161 cgcggtcgtt atagttcaaa atcagtgaca cttaccgcat tgacaagcac gcctcacggg
     8221 agctccaagc ggcgactgag atgtcctaaa tgcacagcga cggattcgcg ctatttagaa
     8281 agagagagca atatttcaag aatgcatgcg tcaattttac gcagactatc tttctagggt
     8341 taatctagct gcatcaggat catatcgtcg ggtctttttt ccggctcagt catcgcccaa
     8401 gctggcgcta tctgggcatc ggggaggaag aagcccgtgc cttttcccgc gaggttgaag
     8461 cggcatggaa agagtttgcc gaggatgact gctgctgcat tgacgttgag cgaaaacgca
     8521 cgtttaccat gatgattcgg gaaggtgtgg ccatgcacgc ctttaacggt gaactgttcg
     8581 ttcaggccac ctgggatacc agttcgtcgc ggcttttccg gacacagttc cggatggtca
     8641 gcccgaagcg catcagcaac ccgaacaata ccggcgacag ccggaactgc cgtgccggtg
     8701 tgcagattaa tgacagcggt gcggcgctgg gatattacgt cagcgaggac gggtatcctg
     8761 gctggatgcc gcagaaatgg acatggatac cccgtgagtt acccggcggg cgcgcttggc
     8821 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     8881 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     8941 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     9001 ttaatgaat
//
