LOCUS       sequence-347866-        8435 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8435)
  AUTHORS   Ohishi H, Shimada S, Uchino S, Li J, Sato Y, Shintani M, Owada H, 
            Ohkawa Y, Pertsinidis A, Yamamoto T, Kimura H, Ochiai H
  TITLE     STREAMING-tag system reveals spatiotemporal relationships between 
            transcriptional regulatory factors and transcriptional activity.
  JOURNAL   Nat Commun. 2022 Dec 20;13(1):7672. doi: 10.1038/s41467-022-35286-2.
  PUBMED    36539402
REFERENCE   2  (bases 1 to 8435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-20"; volume: "13(1):7672. doi"; pages: " 
            10.1038/s41467-022-35286-2"
COMMENT     Sequence Label: eSpCas9-EF
FEATURES             Location/Qualifiers
     source          1..8435
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             790..837
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(865..882)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    871..1078
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   1087..1227
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   1087..1216
                     /label=AAV2 ITR
     rep_origin      1302..1757
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1389..1408)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1599..1620
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1774..1793)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1893..1915
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1953..1971)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2039..2143
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2144..2212,2213..3004)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2362..2381)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3175..3763
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3664..3683
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3825..4073
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     3825..3845
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     3996..4015
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        4274..4559
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     intron          4759..4986
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      4998..5007
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5007..5072
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             5079..5099
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(5124..7661,7662..7664,7665..8126,8127..8129,8130..8297,
                     8298..8300,8301..8435,1..789)
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus pyogenes
                     Type II CRISPR/Cas system, mutated to improve targeting 
                     specificity (Slaymaker et al., 2016)"
                     /label=eSpCas9(1.1)
                     /note="carries the mutations K848A, K1003A, and R1060A"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLADDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPALESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKAPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 agcaaagagt ctatcctgcc caagaggaac agcgataagc tgatcgccag aaagaaggac
       61 tgggacccta agaagtacgg cggcttcgac agccccaccg tggcctattc tgtgctggtg
      121 gtggccaaag tggaaaaggg caagtccaag aaactgaaga gtgtgaaaga gctgctgggg
      181 atcaccatca tggaaagaag cagcttcgag aagaatccca tcgactttct ggaagccaag
      241 ggctacaaag aagtgaaaaa ggacctgatc atcaagctgc ctaagtactc cctgttcgag
      301 ctggaaaacg gccggaagag aatgctggcc tctgccggcg aactgcagaa gggaaacgaa
      361 ctggccctgc cctccaaata tgtgaacttc ctgtacctgg ccagccacta tgagaagctg
      421 aagggctccc ccgaggataa tgagcagaaa cagctgtttg tggaacagca caagcactac
      481 ctggacgaga tcatcgagca gatcagcgag ttctccaaga gagtgatcct ggccgacgct
      541 aatctggaca aagtgctgtc cgcctacaac aagcaccggg ataagcccat cagagagcag
      601 gccgagaata tcatccacct gtttaccctg accaatctgg gagcccctgc cgccttcaag
      661 tactttgaca ccaccatcga ccggaagagg tacaccagca ccaaagaggt gctggacgcc
      721 accctgatcc accagagcat caccggcctg tacgagacac ggatcgacct gtctcagctg
      781 ggaggcgaca aaaggccggc ggccacgaaa aaggccggcc aggcaaaaaa gaaaaagtaa
      841 gaattcctag agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
      901 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
      961 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     1021 gggtggggca ggacagcaag ggggaggatt gggaagagaa tagcaggcat gctggggagc
     1081 ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca
     1141 ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga
     1201 gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc
     1261 tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc
     1321 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgcctt
     1381 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     1441 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     1501 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     1561 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     1621 aacaacactc aactctatct cgggctattc ttttgattta taagggattt tgccgatttc
     1681 ggtctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     1741 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     1801 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     1861 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     1921 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     1981 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     2041 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     2101 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     2161 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     2221 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     2281 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     2341 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     2401 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     2461 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     2521 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     2581 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     2641 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     2701 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     2761 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     2821 ctggtttatt gctgataaat ctggagccgg tgagcgtgga agccgcggta tcattgcagc
     2881 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     2941 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     3001 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     3061 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     3121 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     3181 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     3241 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     3301 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     3361 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     3421 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     3481 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     3541 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     3601 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     3661 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     3721 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     3781 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtgagggc ctatttccca
     3841 tgattccttc atatttgcat atacgataca aggctgttag agagataatt ggaattaatt
     3901 tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg
     3961 ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt
     4021 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac accgggtctt
     4081 cgagaagacc tgtttaagag ctatgctgga aacagcatag caagtttaaa taaggctagt
     4141 ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt tttgttttag agctagaaat
     4201 agcaagttaa aataaggcta gtccgttttt agcgcgtgcg ccaattctgc agacaaatgg
     4261 ctctagaggt acccgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     4321 acccccgccc attgacgtca atagtaacgc caatagggac tttccattga cgtcaatggg
     4381 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     4441 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattgtgcc cagtacatga
     4501 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     4561 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
     4621 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gaatcagagc
     4681 ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag
     4741 cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc
     4801 gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca ggtgagcggg
     4861 cgggacggcc cttctcctcc gggctgtaat tagctgagca agaggtaagg gtttaaggga
     4921 tggttggttg gtggggtatt aatgtttaat tacctggagc acctgcctga aatcactttt
     4981 tttcaggttg gaccggtgcc accatggact ataaggacca cgacggagac tacaaggatc
     5041 atgatattga ttacaaagac gatgacgata agatggcccc aaagaagaag cggaaggtcg
     5101 gtatccacgg agtcccagca gccgacaaga agtacagcat cggcctggac atcggcacca
     5161 actctgtggg ctgggccgtg atcaccgacg agtacaaggt gcccagcaag aaattcaagg
     5221 tgctgggcaa caccgaccgg cacagcatca agaagaacct gatcggagcc ctgctgttcg
     5281 acagcggcga aacagccgag gccacccggc tgaagagaac cgccagaaga agatacacca
     5341 gacggaagaa ccggatctgc tatctgcaag agatcttcag caacgagatg gccaaggtgg
     5401 acgacagctt cttccacaga ctggaagagt ccttcctggt ggaagaggat aagaagcacg
     5461 agcggcaccc catcttcggc aacatcgtgg acgaggtggc ctaccacgag aagtacccca
     5521 ccatctacca cctgagaaag aaactggtgg acagcaccga caaggccgac ctgcggctga
     5581 tctatctggc cctggcccac atgatcaagt tccggggcca cttcctgatc gagggcgacc
     5641 tgaaccccga caacagcgac gtggacaagc tgttcatcca gctggtgcag acctacaacc
     5701 agctgttcga ggaaaacccc atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg
     5761 ccagactgag caagagcaga cggctggaaa atctgatcgc ccagctgccc ggcgagaaga
     5821 agaatggcct gttcggaaac ctgattgccc tgagcctggg cctgaccccc aacttcaaga
     5881 gcaacttcga cctggccgag gatgccaaac tgcagctgag caaggacacc tacgacgacg
     5941 acctggacaa cctgctggcc cagatcggcg accagtacgc cgacctgttt ctggccgcca
     6001 agaacctgtc cgacgccatc ctgctgagcg acatcctgag agtgaacacc gagatcacca
     6061 aggcccccct gagcgcctct atgatcaaga gatacgacga gcaccaccag gacctgaccc
     6121 tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta caaagagatt ttcttcgacc
     6181 agagcaagaa cggctacgcc ggctacattg acggcggagc cagccaggaa gagttctaca
     6241 agttcatcaa gcccatcctg gaaaagatgg acggcaccga ggaactgctc gtgaagctga
     6301 acagagagga cctgctgcgg aagcagcgga ccttcgacaa cggcagcatc ccccaccaga
     6361 tccacctggg agagctgcac gccattctgc ggcggcagga agatttttac ccattcctga
     6421 aggacaaccg ggaaaagatc gagaagatcc tgaccttccg catcccctac tacgtgggcc
     6481 ctctggccag gggaaacagc agattcgcct ggatgaccag aaagagcgag gaaaccatca
     6541 ccccctggaa cttcgaggaa gtggtggaca agggcgcttc cgcccagagc ttcatcgagc
     6601 ggatgaccaa cttcgataag aacctgccca acgagaaggt gctgcccaag cacagcctgc
     6661 tgtacgagta cttcaccgtg tataacgagc tgaccaaagt gaaatacgtg accgagggaa
     6721 tgagaaagcc cgccttcctg agcggcgagc agaaaaaggc catcgtggac ctgctgttca
     6781 agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga ctacttcaag aaaatcgagt
     6841 gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt caacgcctcc ctgggcacat
     6901 accacgatct gctgaaaatt atcaaggaca aggacttcct ggacaatgag gaaaacgagg
     6961 acattctgga agatatcgtg ctgaccctga cactgtttga ggacagagag atgatcgagg
     7021 aacggctgaa aacctatgcc cacctgttcg acgacaaagt gatgaagcag ctgaagcggc
     7081 ggagatacac cggctggggc aggctgagcc ggaagctgat caacggcatc cgggacaagc
     7141 agtccggcaa gacaatcctg gatttcctga agtccgacgg cttcgccaac agaaacttca
     7201 tgcagctgat ccacgacgac agcctgacct ttaaagagga catccagaaa gcccaggtgt
     7261 ccggccaggg cgatagcctg cacgagcaca ttgccaatct ggccggcagc cccgccatta
     7321 agaagggcat cctgcagaca gtgaaggtgg tggacgagct cgtgaaagtg atgggccggc
     7381 acaagcccga gaacatcgtg atcgaaatgg ccagagagaa ccagaccacc cagaagggac
     7441 agaagaacag ccgcgagaga atgaagcgga tcgaagaggg catcaaagag ctgggcagcc
     7501 agatcctgaa agaacacccc gtggaaaaca cccagctgca gaacgagaag ctgtacctgt
     7561 actacctgca gaatgggcgg gatatgtacg tggaccagga actggacatc aaccggctgt
     7621 ccgactacga tgtggaccat atcgtgcctc agagctttct ggccgacgac tccatcgaca
     7681 acaaggtgct gaccagaagc gacaagaacc ggggcaagag cgacaacgtg ccctccgaag
     7741 aggtcgtgaa gaagatgaag aactactggc ggcagctgct gaacgccaag ctgattaccc
     7801 agagaaagtt cgacaatctg accaaggccg agagaggcgg cctgagcgaa ctggataagg
     7861 ccggcttcat caagagacag ctggtggaaa cccggcagat cacaaagcac gtggcacaga
     7921 tcctggactc ccggatgaac actaagtacg acgagaatga caagctgatc cgggaagtga
     7981 aagtgatcac cctgaagtcc aagctggtgt ccgatttccg gaaggatttc cagttttaca
     8041 aagtgcgcga gatcaacaac taccaccacg cccacgacgc ctacctgaac gccgtcgtgg
     8101 gaaccgccct gatcaaaaag taccctgcgc tggaaagcga gttcgtgtac ggcgactaca
     8161 aggtgtacga cgtgcggaag atgatcgcca agagcgagca ggaaatcggc aaggctaccg
     8221 ccaagtactt cttctacagc aacatcatga actttttcaa gaccgagatt accctggcca
     8281 acggcgagat ccggaaggcg cctctgatcg agacaaacgg cgaaaccggg gagatcgtgt
     8341 gggataaggg ccgggatttt gccaccgtgc ggaaagtgct gagcatgccc caagtgaata
     8401 tcgtgaaaaa gaccgaggtg cagacaggcg gcttc
//
