LOCUS       sequence-347877-        7291 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 module containing 35S-deltaPTAC5-cYFP_P3 F.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7291)
  AUTHORS   Velay F, Soula M, Mehrez M, Belbachir C, D'Alessandro S, Laloi C, 
            Crete P, Field B
  TITLE     MoBiFC: development of a modular bimolecular fluorescence 
            complementation toolkit for the analysis of chloroplast 
            protein-protein interactions.
  JOURNAL   Plant Methods. 2022 May 26;18(1):69. doi: 
            10.1186/s13007-022-00902-1.
  PUBMED    35619173
REFERENCE   2  (bases 1 to 7291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2022-05-26"; volume: "18(1):69. doi"; pages: " 
            10.1186/s13007-022-00902-1"
COMMENT     Sequence Label: pLBT0110
FEATURES             Location/Qualifiers
     source          1..7291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1023..1368
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1311..1331
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     2581..2602
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2645..2734
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     polyA_signal    2775..2949
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    2994..3018
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(3174..4322)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(4658..5246)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4738..4757)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5417..6208,6209..6277))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6040..6059
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6278..6382)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6469..7100
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    7192..7216
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tctgtgaaga caaactagaa ttcgagctcg gaggaattcc aatcccacaa aaatctgagc
       61 ttaacagcac agttgctcct ctcagagcag aatcgggtat tcaacaccct catatcaact
      121 actacgttgt gtataacggt ccacatgccg gtatatacga tgactggggt tgtacaaagg
      181 cggcaacaaa cggcgttccc ggagttgcac acaagaaatt tgccactatt acagaggcaa
      241 gagcagcagc tgacgcgtac acaacaagtc agcaaacaga caggttgaac ttcatcccca
      301 aaggagaagc tcaactcaag cccaagagct ttgctaaggc cctaacaagc ccaccaaagc
      361 aaaaagccca ctggctcacg ctaggaacca aaaggcccag cagtgatcca gccccaaaag
      421 agatctcctt tgccccggag attacaatgg acgatttcct ctatctttac gatctaggaa
      481 ggaagttcga aggtgaaggt gacgacacta tgttcaccac tgataatgag aaggttagcc
      541 tcttcaattt cagaaagaat gctgacccac agatggttag agaggcctac gcagcaagtc
      601 tcatcaagac gatctacccg agtaacaatc tccaggagat caaatacctt cccaagaagg
      661 ttaaagatgc agtcaaaaga ttcaggacta attgcatcaa gaacacagag aaagacatat
      721 ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt cataaaccaa
      781 ggcaagtaat agagattgga gtctctaaaa aggtagttcc tactgaatct aaggccatgc
      841 atggagtcta agattcaaat cgaggatcta acagaactcg ccgtcaagac tggcgaacag
      901 ttcatacaga gtcttttacg actcaatgac aagaagaaaa tcttcgtcaa catggtggag
      961 cacgacactc tggtctactc caaaaatgtc aaagatacag tctcagaaga tcaaagggct
     1021 attgagactt ttcaacaaag gataatttcg ggaaacctcc tcggattcca ttgcccagct
     1081 atctgtcact tcatcgaaag gacagtagaa aaggaaggtg gctcctacaa atgccatcat
     1141 tgcgataaag gaaaggctat cattcaagat ctctctgccg acagtggtcc caaagatgga
     1201 cccccaccca cgaggagcat cgtggaaaaa gaagaggttc caaccacgtc tacaaagcaa
     1261 gtggattgat gtgacatctc cactgacgta agggatgacg cacaatccca ctatccttcg
     1321 caagaccctt cctctatata aggaagttca tttcatttgg agaggacacg ctcgagtata
     1381 agagctcatt tttacaacaa ttaccaacaa caacaaacaa caaacaacat tacaattaca
     1441 tttacaatta tcgatacaat ggcttcctct atgctctctt ccgctactat ggttgcctct
     1501 ccggctcagg ccactatggt cgctcctttc aacggactta agtcctccgc tgccttccca
     1561 gccacccgca aggctaacaa cgacattact tccatcacaa gcaacggcgg aagagttaac
     1621 tgcatgcagg tcatttatat ttcttctttc acttttttat tattccatat gatttttttc
     1681 ggttctttct tcgaatctac ataaactaat atcattggaa aaatcgaaaa aataggtgtg
     1741 gcctccgatt ggaaagaaga agtttgagac tctctcttac cttcctgacc ttaccgattc
     1801 cgaaggttgc ttctccactc aaaatcccga ccgcgaagag gtccggtggc tccgggaaga
     1861 gcagagatgg attcgcgagg agcaacgatg gattcgtgaa gaacagagat ggatacgcga
     1921 acgtgaatcg cttctacaag agatttcgga tctacagctc agaattcaat ccctagagtc
     1981 acgaaattcg caattgggga attctattcc cgatacgatt tcgaatatcg ctgctttgct
     2041 tcaggttttg aaggagaaga atcggatttc tgagagtgga ttgagcgcaa cgccgatggt
     2101 attggagagt acgagagaac aaattgttga ggaggtggaa gaagaagaga agcgagtgat
     2161 tattgctgaa gagaaagtta gggtttcgga gccggtgaag aagatcaaga ggaggatatt
     2221 gaaagttgga agcgaaggcg acgatgttca agctttgcag gaagctctgt tgaaattagg
     2281 attctattcg ggcgaagagg atatggagtt ctcgagcttt tcaagtggga ctgcaagtgc
     2341 tgttaagact tggcaagcat cgcttggggt ccgtgaggat ggggtaatga cagcagagct
     2401 tcttcagagg ttgttcatgg atgaggacgt agagacagat aagtcttcgg gcagcgtgca
     2461 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     2521 caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     2581 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     2641 caagtaccca tacgatgttc ctgactatgc gggctatccc tatgacgtcc cggactatgc
     2701 aggatcctat ccatatgacg ttccagatta cgctgcttaa gcttctctag ctagagtcga
     2761 tcgacaagct cgagtttctc cataataatg tgtgagtagt tcccagataa gggaattagg
     2821 gttcctatag ggtttcgctc atgtgttgag catataagaa acccttagta tgtatttgta
     2881 tttgtaaaat acttctatca ataaaatttc taattcctaa aaccaaaatc cagtactaaa
     2941 atccagatcg ctttacttgt cttctgcacg aagtggttta aactatcagt gtttgacagg
     3001 atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa
     3061 agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc
     3121 ccagatcagg cgctggctgc tgaaccccca gccggaactg accccacaag gccctagcgt
     3181 ttgcaatgca ccaggtcatc attgacccag gcgtgttcca ccaggccgct gcctcgcaac
     3241 tcttcgcagg cttcgccgac ctgctcgcgc cacttcttca cgcgggtgga atccgatccg
     3301 cacatgaggc ggaaggtttc cagcttgagc gggtacggct cccggtgcga gctgaaatag
     3361 tcgaacatcc gtcgggccgt cggcgacagc ttgcggtact tctcccatat gaatttcgtg
     3421 tagtggtcgc cagcaaacag cacgacgatt tcctcgtcga tcaggacctg gcaacgggac
     3481 gttttcttgc cacggtccag gacgcggaag cggtgcagca gcgacaccga ttccaggtgc
     3541 ccaacgcggt cggacgtgaa gcccatcgcc gtcgcctgta ggcgcgacag gcattcctcg
     3601 gccttcgtgt aataccggcc attgatcgac cagcccaggt cctggcaaag ctcgtagaac
     3661 gtgaaggtga tcggctcgcc gataggggtg cgcttcgcgt actccaacac ctgctgccac
     3721 accagttcgt catcgtcggc ccgcagctcg acgccggtgt aggtgatctt cacgtccttg
     3781 ttgacgtgga aaatgacctt gttttgcagc gcctcgcgcg ggattttctt gttgcgcgtg
     3841 gtgaacaggg cagagcgggc cgtgtcgttt ggcatcgctc gcatcgtgtc cggccacggc
     3901 gcaatatcga acaaggaaag ctgcatttcc ttgatctgct gcttcgtgtg tttcagcaac
     3961 gcggcctgct tggcctcgct gacctgtttt gccaggtcct cgccggcggt ttttcgcttc
     4021 ttggtcgtca tagttcctcg cgtgtcgatg gtcatcgact tcgccaaacc tgccgcctcc
     4081 tgttcaagac gacgcgaacg ctccacggcg gccgatggcg cgggcagggc agggggagcc
     4141 agttgcacgc tgtcgcgctc gatcttggcc gtagcttgct ggaccatcga gccgacggac
     4201 tggaaggttt cgcggggcgc acgcatgacg gtgcggcttg cgatggtttc ggcatcctcg
     4261 gcggaaaacc ccgcgtcgat cagttcttgc ctgtatgcct tccggtcaaa cgtccgattc
     4321 attcaccctc cttgcgggat tgccccgact cacgccgggg caatgtgccc ttattcctga
     4381 tttgacccgc ctggtgcctt ggtgtccaga taatccacct tatcggcaat gaagtcggtc
     4441 ccgtagaccg tctggccgtc cttctcgtac ttggtattcc gaatcttgcc ctgcacgaat
     4501 accagcgacc ccttgcccaa atacttgccg tgggcctcgg cctgagagcc aaaacacttg
     4561 atgcggaaga agtcggtgcg ctcctgcttg tcgccggcat cgttgcgcca catctaggat
     4621 ctgccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     4681 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     4741 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     4801 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     4861 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     4921 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     4981 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     5041 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     5101 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     5161 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     5221 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     5281 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     5341 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     5401 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     5461 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     5521 gcttaccatc tggccccagt gctgcaatga taccgcgaga accacgctca ccggctccag
     5581 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     5641 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     5701 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     5761 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     5821 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     5881 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     5941 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     6001 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     6061 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     6121 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     6181 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     6241 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     6301 gaagcattta tcaaggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     6361 ataaacaaat aggggttccg cgcacgaatt ggccagcgct gccatttttg gggtgaggcc
     6421 gttcgcggcc gaggggcgca gcccctgggg ggatgggagg cccgcgttag cgggccggga
     6481 gggttcgaga agggggggca ccccccttcg gcgtgcgcgg tcacgcgcac agggcgcagc
     6541 cctggttaaa aacaaggttt ataaatattg gtttaaaagc aggttaaaag acaggttagc
     6601 ggtggccgaa aaacgggcgg aaacccttgc aaatgctgga ttttctgcct gtggacagcc
     6661 cctcaaatgt caataggtgc gcccctcatc tgtcagcact ctgcccctca agtgtcaagg
     6721 atcgcgcccc tcatctgtca gtagtcgcgc ccctcaagtg tcaataccgc agggcactta
     6781 tccccaggct tgtccacatc atctgtggga aactcgcgta aaatcaggcg ttttcgccga
     6841 tttgcgaggc tggccagctc cacgtcgccg gccgaaatcg agcctgcccc tcatctgtca
     6901 acgccgcgcc gggtgagtcg gcccctcaag tgtcaacgtc cgcccctcat ctgtcagtga
     6961 gggccaagtt ttccgcgagg tatccacaac gccggcggcc gcggtgtctc gcacacggct
     7021 tcgacggcgt ttctggcgcg tttgcagggc catagacggc cgccagccca gcggcgaggg
     7081 caaccagccc ggtgagcgtc gcaaaggaga tcctgatctg actgatgggc tgcctgtatc
     7141 gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg gtggcaggat
     7201 atattgtggt gtaaacaaat tgacgcttag acaacttaat aacacattgc ggacgttttt
     7261 aatgtactgg ggtggatgca gtgggcccca c
//
