LOCUS       sequence-347882-        8539 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gram-Positive Bacterial Shuttle Vector for Nisin-Controlled 
            Inducible Expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8539)
  AUTHORS   Bryan EM, Bae T, Kleerebezem M, Dunny GM
  TITLE     Improved vectors for nisin-controlled expression in gram-positive 
            bacteria.
  JOURNAL   Plasmid. 2000 Sep;44(2):183-90.
  PUBMED    10964628
REFERENCE   2  (bases 1 to 8539)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid."; 
            date: "2000-09"; volume: "44(2)"; pages: "183-90"
COMMENT     Sequence Label: pMSP3545
FEATURES             Location/Qualifiers
     source          1..8539
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      7694..8282
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8183..8202
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8436..8453
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatacgact cactataggg agaccggcct cgagaagctc gcgttatcgg tcctttaatt
       61 ggtggacaaa tttacattag tctcggacat tctgctcccg cccttatggg atttatcttc
      121 cttattctcg ctttgattgt tctatcgaaa gcgaaatcaa acgaataatt tataaataaa
      181 aaaaccacct ctaaaaggtg atttttattt ataaattaca atttttcagc aatatcagta
      241 attgctttat caactgctgc tttttggcta tcaatcaaag caacacgtgc tgtaatttgt
      301 ttaattgcca tttcaattga acgtttcaag ccttggtttt ctaattttgg ttcaaagaaa
      361 gcttgagctc tctagaacta gtggtaccgc atgcctgcag tacccatggt gagtgcctcc
      421 ttataattta ttttgtagtt ccttcgaacg aaatcattgt atctaacaaa cttcagaatt
      481 taatcagagc cgtttattat gctcgcgtta tcgacaataa tattattacc aatactttct
      541 caagatagaa ttaagactgt tttagatttg ttaatgtttc tattgtcagt atagttataa
      601 gactagatct gatccgtagc ggttttcaaa atttgcaacc aggaatgaat tactatccct
      661 tttatcaaga agcgcacaaa aagaaaaacg aaatgataca ccaatcagtg caaaaaaaga
      721 tataatggga gataagacgg ttcgtgttcg tgctgacttg caccatatca taaaaatcga
      781 aacagcaaag aatggcggaa acgtaaaaga agttatggaa ataagactta gaagcaaact
      841 taagagtgtg ttgatagtgc agtatcttaa aattttgtat aataggaatt gaagttaaat
      901 tagatgctaa aaatttgtaa ttaagaagga gtgattacat gaacaaaaat ataaaatatt
      961 ctcaaaactt tttaacgagt gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa
     1021 aagaaaccga taccgtttac gaaattggaa caggtaaagg gcatttaacg acgaaactgg
     1081 ctaaaataag taaacaggta acgtctattg aattagacag tcatctattc aacttatcgt
     1141 cagaaaaatt aaaactgaat actcgtgtca ctttaattca ccaagatatt ctacagtttc
     1201 aattccctaa caaacagagg tataaaattg ttgggagtat tccttaccat ttaagcacac
     1261 aaattattaa aaaagtggtt tttgaaagcc atgcgtctga catctatctg attgttgaag
     1321 aaggattcta caagcgtacc ttggatattc accgaacact agggttgctc ttgcacactc
     1381 aagtctcgat tcagcaattg cttaagctgc cagcggaatg ctttcatcct aaaccaaaag
     1441 taaacagtgt cttaataaaa cttacccgcc ataccacaga tgttccagat aaatattgga
     1501 agctatatac gtactttgtt tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta
     1561 aaaatcagtt tcatcaagca atgaaacacg ccaaagtaaa caatttaagt accgttactt
     1621 atgagcaagt attgtctatt tttaatagtt atctattatt taacgggagg aaataattct
     1681 atgagtcgct tttgtaaatt tggaaagtta cacgttacta aagggaatgt agataaatta
     1741 ttaggtatac tactgacagc ttccaaggag ctaaagaggt ccctagcgct tagaatcgct
     1801 ttaggaaaca cgatccagtc caataatcgt cgataaaaac ttttgaaaaa ggttggtgaa
     1861 attacctact tttggaataa tcacaaatca caagtgatta atcacaaatc acaagtgatt
     1921 aatcacttgt ttattaagat attaaaagct ataatttaaa taaagcgtga attttattac
     1981 acaaaaagag gggggagaaa cttggaacta gcatttagag aaagcttaaa aaagatgaga
     2041 ggtaccaaat caaaagaaaa attctcccaa gaattagaaa tgagtagatc aaattattca
     2101 cgaatagaat caggaaaatc agatccaacc ataaaaacac tagaacaaat tgcaaagtta
     2161 actaactcaa cgctagtagt ggatttaatc ccaaatgagc caacagaacc agaaccagaa
     2221 acagaatcag aacaagtaac attggattta gaaatggaag aagaaaaaag caatgacttc
     2281 gtgtgaataa tgcacgaaat cgttgcttat tttttttaaa agcggtatac tagatataac
     2341 gaaacaacga actgaataga aacgaaaaaa gagccatgac acatttataa aatgtttgac
     2401 gacattttat aaatgcatag cccgataaga ttgccaaacc aacgcttatc agttagtcag
     2461 atgaactctt ccctcgtaag aagttattta attaactttg tttgaagacg gtatataacc
     2521 gtactatcat tatataggga aatcagagag ttttcaagta tctaagctac tgaatttaag
     2581 aattgttaag caatcaatcg gaaatcgttt gattgctttt tttgtattca tttatagaag
     2641 gtggagtttg tatgaatcat gatgaatgta aaacttatat aaaaaatagt ttattggaga
     2701 taagaaaatt agcaaatatc tatacactag aaacgtttaa gaaagagtta gaaaagagaa
     2761 atatctactt agaaacaaaa tcagataagt atttttcttc ggagggggaa gattatatat
     2821 ataagttaat agaaaataac aaaataattt attcgattag tggaaaaaaa ttgacttata
     2881 aaggaaaaaa atctttttca aaacatgcaa tattgaaaca gttgaatgaa aaagcaaacc
     2941 aagttaatta aacaacctat tttataggat ttataggaaa ggagaacagc tgaatgaata
     3001 tcccttttgt tgtagaaact gtgcttcatg acggcttgtt aaagtacaaa tttaaaaata
     3061 gtaaaattcg ctcaatcact accaagccag gtaaaagcaa aggggccatt tttgcgtatc
     3121 gctcaaaatc aagcatgatt ggcggtcgtg gtgttgttct gacttccgag gaagcgattc
     3181 aagaaaatca agatacattt acacattgga cacccaacgt ttatcgttat ggaacgtatg
     3241 cagacgaaaa ccgttcatac acgaaaggac attctgaaaa caatttaaga caaatcaata
     3301 ccttctttat tgattttgat attcacacgg caaaagaaac tatttcagca agcgatattt
     3361 taacaaccgc tattgattta ggttttatgc ctactatgat tatcaaatct gataaaggtt
     3421 atcaagcata ttttgtttta gaaacgccag tctatgtgac ttcaaaatca gaatttaaat
     3481 ctgtcaaagc agccaaaata atttcgcaaa atatccgaga atattttgga aagtctttgc
     3541 cagttgatct aacgtgtaat cattttggta ttgctcgcat accaagaacg gacaatgtag
     3601 aattttttga tcctaattac cgttattctt tcaaagaatg gcaagattgg tctttcaaac
     3661 aaacagataa taagggcctt actcgttcaa gtctaacggt tttaagcggt acagaaggca
     3721 aaaaacaagt agatgaaccc tggtttaatc tcttattgca cgaaacgaaa ttttcaggag
     3781 aaaagggttt aatagggcgt aataacgtca tgtttaccct ctctttagcc tactttagtt
     3841 caggctattc aatcgaaacg tgcgaatata atatgtttga gtttaataat cgattagatc
     3901 aacccttaga agaaaaagaa gtaatcaaaa ttgttagaag tgcctattca gaaaactatc
     3961 aaggggctaa tagggaatac attaccattc tttgcaaagc ttgggtatca agtgatttaa
     4021 ccagtaaaga tttatttgtc cgtcaagggt ggtttaaatt caagaaaaaa agaagcgaac
     4081 gtcaacgtgt tcatttgtca gaatggaaag aagatttaat ggcttatatt agcgaaaaaa
     4141 gcgatgtata caagccttat ttagtgacga ccaaaaaaga gattagagaa gtgctaggca
     4201 ttcctgaacg gacattagat aaattgctga aggtactgaa ggcgaatcag gaaattttct
     4261 ttaagatcaa accaggaaga aatggtggca ttcaacttgc tagtgttaaa tcattgttgc
     4321 tatcgatcat taaagtaaaa aaagaagaaa aagaaagcta tataaaggcg ctgacaaatt
     4381 cttttgactt agagcataca ttcattcaag agactttaaa caagctagca gaacgcccta
     4441 aaacggacac acaactcgat ttgtttagct atgatacagg ctgaaaataa aacccgcact
     4501 atgccattac atttatatct atgatacgtg tttgtttttt ctttgctgtt tagcgaatga
     4561 ttagcagaaa tatacagagt aagattttaa ttaattatta gggggagaag gagagagtag
     4621 cccggaaact tttagttggc ttggactgaa cgaagtgagg gaaaggctac taaaacgtcg
     4681 aggggcagtg agagcgaagc gaacacttga ttttttaatt ttctatcttt tataggtcat
     4741 tagagtatac ttatttgtcc tataaactat ttagcagcat aatagattta ttgaataggt
     4801 catttaagtt gagcatatta gaggaggaaa atcttggaga aatatttgaa gaacccgatt
     4861 acatggattg gattagttct tgtggttacg tggtttttaa ctaaaagtag tgaatttttg
     4921 atttttggtg tgtgtgtctt gttgttagta tttgctagtc aaagtgatta aatagaattc
     4981 tcatgtttga cagcttatca tcggagctcc gatgataagc tgtcaaacat gagaattccc
     5041 ggctttaggt atagtgtgta tctcaatcct tggtatattg aaaagaaaga ctaaaaattg
     5101 atagattata tttcttcaga atgaatggta taatgaagta atgagtacta aacaatcgga
     5161 ggtaaagtgg tgtataaaat tttaatagtt gatgatgatc aggaaatttt aaaattaatg
     5221 aagacagcat tagaaatgag aaactatgaa gttgcgacgc atcaaaacat ttcacttccc
     5281 ttggatatta ctgattttca gggatttgat ttgattttgt tagatatcat gatgtcaaat
     5341 attgaaggga cagaaatttg taaaaggatt cgcagagaaa tatcaactcc aattatcttt
     5401 gttagtgcga aagatacaga agaggatatt ataaacggct taggtattgg tggggatgac
     5461 tatattacta agccttttag ccttaaacag ttggttgcaa aagtggaagc aaatataaag
     5521 cgagaggaac gcaataaaca tgcagttcat gttttttcag agattcgtag agatttagga
     5581 ccaattacat tttatttaga agaaaggcga gtctgtgtca atggtcaaac aattccactg
     5641 acttgtcgcg aatacgatat tcttgaatta ctatcacaac gaacttctaa agtttatacg
     5701 agagaggata tttatgatga cgtatatgat gaatattcta atgcactttt tcggtcaatc
     5761 tcggaatata tttatcagat taggagtaag tttgcaccat acgatattaa tccgataaaa
     5821 acggttcggg gacttgggta tcagtggcat gggtaaaaaa tattcaatgc gtcgacggat
     5881 atggcaagct gtcattgaaa ttatcatagg tacttgtcta cttatcctgt tgttactggg
     5941 cttgactttc tttctacgac aaattggaca aatcagtggt tcagaaacta ttcgtttatc
     6001 tttagattca gataatttaa ctatttctga tatcgaacgt gatatgaaac actacccata
     6061 tgattatatt atttttgaca atgatacaag taaaattttg ggaggacatt atgtcaagtc
     6121 ggatgtacct agttttgtag cttcaaaaca gtcttcacat aatattacag aaggagaaat
     6181 tacttatact tattcaagca ataagcattt ttcagttgtt ttaagacaaa acagtatgcc
     6241 tgaatttaca aatcatacgc ttcgttcaat ttcttataat caatttactt accttttctt
     6301 ttttcttggt gaaataatac tcattatttt ttctgtctat catctcatta gagaattttc
     6361 taagaatttt caagccgttc aaaagattgc attgaagatg ggggaaataa ctacttttcc
     6421 tgaacaagag gaatcaaaaa ttattgaatt tgatcaggtt ctgaataact tatattcgaa
     6481 aagtaaggag ttagctttcc ttattgaagc ggagcgtcat gaaaaacatg atttatcctt
     6541 ccaggttgct gcactttcac atgatgttaa gacaccttta acagtattaa aaggaaatat
     6601 tgaactgcta gagatgactg aagtaaatga acaacaagct gattttattg agtcaatgaa
     6661 aaatagtttg actgtttttg acaagtattt taacacaatg attagttata caaaactttt
     6721 gaatgatgaa aatgattaca aagcgataat ctccctggag gattttttga tagatttatc
     6781 agttgagttg gaagagttgt caacaactta tcaagtggat tatcagctag ttaaaaaaac
     6841 agatttaacc actttttacg gaaatacatt agctttaagt cgagcactta tcaatatctt
     6901 tgttaatgcc tgtcagtatg ctaaagaggg tgaaaaaata gtcggtttga gtatttatga
     6961 tgatgaaaaa tatctctatt ttgaaatctg gaataatggt catccttttt ctgaacaagc
     7021 aaaaaaaaat gctggaaaac tatttttcac agaagatact ggacgtagtg ggaaacacta
     7081 tgggattgga ctatcttttg ctcaaggtgt agctttaaaa catcaaggaa acttaattct
     7141 cagtaatcct caaaaaggtg gggcagaagt tatcctaaaa ataaaaaagt aatttagtaa
     7201 tctctaagga ttactttttt tgtttcggca aactattaac tggcgaacta cttactctag
     7261 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     7321 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     7381 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     7441 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     7501 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     7561 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     7621 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     7681 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     7741 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     7801 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     7861 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     7921 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     7981 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     8041 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     8101 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     8161 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     8221 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     8281 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     8341 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     8401 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     8461 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcaggt
     8521 taacctggct tatcgaaat
//
