LOCUS       sequence-34789-e         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  TITLE     Cookson Lab Plasmids
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti6-DJ1-V5-D149A
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(13..54)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    107..131
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(849..873)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(870..921)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(899..919)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ctaaccggta cgcgtagaat cgagaccgag gagagggtta gggataggct taccttcgaa
       61 ccgcgggccc tctagactcg agcggccgcc actgtgctgg atatcaacca ctttgtacaa
      121 gaaagctggg tcgaattcgc ccttcttgtc tttaagaaca agtggagcct tcacttgagc
      181 cgccacctcc ttgccattca gggcttcaac aattgcaagc gcaaactcga agctggtccc
      241 aggcccccgg cttgtaagaa tcaggccggc tttttccaca cgattctcag agtaggtgta
      301 atgacctcca ttcatcattt tgtctttagc aagagggtgt gttgtaactt tacttccaaa
      361 acctatttca tgagccaaca gagcagtagg acctgcacag atggcggcta tcaggccctt
      421 ccggttttcc tgctccttca gtatctcctt cacagcagca gactcagata aattctgtgc
      481 gcccagatta cctcctggta gaaccaccac atcatatggt ccctcttttt ttgcatcttc
      541 aaggctggca tcaggacaaa tgaccacatc acggctacac tgtactgggt cttttccagc
      601 caggcctgca acggtgacct taatcccagc tcgcctcatg acatctacag ggatgaccgt
      661 ctccatttcc tctgctcctt tagccaggat gaccagagct cttttggaag ccataagggc
      721 gaattcggag cctgcttttt tgtacaaact tgttgatatc tgcagaattc caccacactg
      781 gactagtgga tcctctagag tcggtgtctt ctatggaggt caaaacagcg tggatggcgt
      841 ctccaggcga tctgacggtt cactaaacga gctctgctta tatagacctc ccaccgtaca
      901 cgcctaccgc ccatttgcgt caatggggc
//
