LOCUS       sequence-34790-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  TITLE     Cookson Lab Plasmids
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti6-DJ1-V5-D149A
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    73..97
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(695..719)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             772..813
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 ttttgacctc catagaagac accgactcta gaggatccac tagtccagtg tggtggaatt
       61 ctgcagatat caacaagttt gtacaaaaaa gcaggctccg aattcgccct tatggcttcc
      121 aaaagagctc tggtcatcct ggctaaagga gcagaggaaa tggagacggt catccctgta
      181 gatgtcatga ggcgagctgg gattaaggtc accgttgcag gcctggctgg aaaagaccca
      241 gtacagtgta gccgtgatgt ggtcatttgt cctgatgcca gccttgaaga tgcaaaaaaa
      301 gagggaccat atgatgtggt ggttctacca ggaggtaatc tgggcgcaca gaatttatct
      361 gagtctgctg ctgtgaagga gatactgaag gagcaggaaa accggaaggg cctgatagcc
      421 gccatctgtg caggtcctac tgctctgttg gctcatgaaa taggttttgg aagtaaagtt
      481 acaacacacc ctcttgctaa agacaaaatg atgaatggag gtcattacac ctactctgag
      541 aatcgtgtgg aaaaagccgg cctgattctt acaagccggg ggcctgggac cagcttcgag
      601 tttgcgcttg caattgttga agccctgaat ggcaaggagg tggcggctca agtgaaggct
      661 ccacttgttc ttaaagacaa gaagggcgaa ttcgacccag ctttcttgta caaagtggtt
      721 gatatccagc acagtggcgg ccgctcgagt ctagagggcc cgcggttcga aggtaagcct
      781 atccctaacc ctctcctcgg tctcgattct acgcgtaccg gtttagtaat gagtttggaa
//
