LOCUS       sequence-347943-        4811 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express mCerulean3 fused to the N-terminus of the Bcl-2 family 
            chimera protein, Bcl-XL-ActA: Bcl-XL with its membrane binding 
            region swapped for ActA (mitochondrial targeting sequence)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4811)
  TITLE     David Andrews Lab plasmids
REFERENCE   2  (bases 1 to 4811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCerulean3-BclXL-ActA-s2193
FEATURES             Location/Qualifiers
     source          1..4811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    33..154
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(44..63)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     98..117
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      342..929
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     830..849
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        1051..1242
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(1074..1094)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     regulatory      2364..2373
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2370..2372,2373..2375,2376..3086)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2415..2436)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2676..2695)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3023..3044
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3836..4280
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     promoter        4314..4360
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        4352..4370
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cttcgaactc gaggctagct ggccagacat gataagatac attgatgagt ttggacaaac
       61 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
      121 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
      181 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
      241 tggtatggaa atgttaatta actagccatg accaaaatcc cttaacgtga gttttcgttc
      301 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
      361 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
      421 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
      481 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
      541 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
      601 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
      661 acgggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
      721 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
      781 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
      841 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
      901 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
      961 tggccttttg ctggcctttt gctcacatgt tcttaattaa cctgcagggc ctgaaataac
     1021 ctctgaaaga ggaacttggt taggtacctt ctgaggctga aagaaccagc tgtggaatgt
     1081 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     1141 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     1201 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccactagttc cgccagagcg
     1261 cgcgagggcc tccagcggcc gcccctcccc cacagcaggg gcggggtccc gcgcccaccg
     1321 gaaggagcgg gctcggggcg ggcggcgctg attggccggg gcgggcctga cgccgacgcg
     1381 gctataagag accacaagcg acccgcaggg ccagacgttc ttcgccgaag cttgccgtca
     1441 gaacgcaggt gaggggcggg tgtggcttcc gcgggccgcc gagctggagg tcctgctccg
     1501 agcgggccgg gccccgctgt cgtcggcggg gattagctgc gagcattccc gcttcgagtt
     1561 gcgggcggcg cgggaggcag agtgcgaggc ctagcggcaa ccccgtagcc tcgcctcgtg
     1621 tccggcttga ggcctagcgt ggtgtccgcg ccgccgccgc gtgctactcc ggccgcactc
     1681 tggtcttttt tttttttgtt gttgttgccc tgctgccttc gattgccgtt cagcaatagg
     1741 ggctaacaaa gggagggtgc ggggcttgct cgcccggagc ccggagaggt catggttggg
     1801 gaggaatgga gggacaggag tggcggctgg ggcccgcccg ccttcggagc acatgtccga
     1861 cgccacctgg atggggcgag gcctggggtt tttcccgaag caaccaggct ggggttagcg
     1921 tgccgaggcc atgtggcccc agcacccggc acgatctggc ttggcggcgc cgcgttgccc
     1981 tgcctcccta actagggtga ggccatcccg tccggcacca gttgcgtgcg tggaaagatg
     2041 gccgctcccg ggccctgttg caaggagctc aaaatggagg acgcggcagc ccggtggagc
     2101 gggcgggtga gtcacccaca caaaggaaga gggcctggtc cctcaccggc tgctgcttcc
     2161 tgtgaccccg tggtcctatc ggccgcaata gtcacctcgg gcttttgagc acggctagtc
     2221 gcggcggggg gaggggatgt aatggcgttg gagtttgttc acatttggtg ggtggagact
     2281 agtcaggcca gcctggcgct ggaagtcatt tttggaattt gtccccttga gttttgagcg
     2341 gagctaattc aaagcaaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg
     2401 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     2461 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     2521 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgagctgg ggcgtgcagt
     2581 gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     2641 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     2701 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     2761 tcaaggagga cggcaacatc ctggggcaca agctggagta caacgccatc cacggcaacg
     2821 tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcggc ctcaactgca
     2881 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     2941 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccaag ctgagcaaag
     3001 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     3061 ctctcggcat ggacgagctg tacaagtccg gactcagatc cacgcgtatg agccaaagca
     3121 accgggagct ggtggttgac tttctctcct acaagctttc ccagaaagga tacagctgga
     3181 gtcagtttag tgatgtggaa gagaacagga ctgaggcccc agaagggact gaatcggaga
     3241 tggagacccc cagtgccatc aatggcaacc catcctggca cctggcagac agccccgcgg
     3301 tgaatggagc cactggccac agcagcagtt tggatgcccg ggaggtgatc cccatggcag
     3361 cagtaaagca agcgctgagg gaggcaggcg acgagtttga actgcggtac cggcgggcat
     3421 tcagtgacct gacatcccag ctccacatca ccccagggac agcatatcag agctttgaac
     3481 aggtagtgaa tgaactcttc cgggatgggg taaactgggg tcgcattgtg gcctttttct
     3541 ccttcggcgg ggcactgtgc gtggaaagcg tagacaagga gatgcaggta ttggtgagtc
     3601 ggatcgcagc ttggatggcc acttacctga atgaccacct agagccttgg atccaggaga
     3661 acggcggctg ggatactttt gtggaactct atgggaacaa tgcagcagcc gagagccgaa
     3721 agggccagga acgcttcctt aagctgattc tcgccatgct ggcaattggg gtgttctccc
     3781 tcggcgcctt cattaagatc attcagctcc ggaagaacaa ttagtcgacc ctaggagcag
     3841 gtttccccaa tgacacaaaa cgtgcaactt gaaactccgc ctggtctttc caggtctaga
     3901 ggggtaacac tttgtactgc gtttggctcc acgctcgatc cactggcgag tgttagtaac
     3961 agcactgttg cttcgtagcg gagcatgacg gccgtgggaa ctcctccttg gtaacaagga
     4021 cccacggggc caaaagccac gcccacacgg gcccgtcatg tgtgcaaccc cagcacggcg
     4081 actttactgc gaaacccact ttaaagtgac attgaaactg gtacccacac actggtgaca
     4141 ggctaaggat gcccttcagg taccccgagg taacacgcga cactcgggat ctgagaaggg
     4201 gactggggct tctataaaag cgctcggttt aaaaagcttc tatgcctgaa taggtgaccg
     4261 gaggtcggca cctttccttt gcaattactg accctatgaa tacaactgac tgtttgacaa
     4321 ttaatcatcg gcatagtata tcggcatagt ataatacgac tcactatagg agggccacca
     4381 tgaagacctt caacatctct cagcaggatc tggagctggt ggaggtcgcc actgagaaga
     4441 tcaccatgct ctatgaggac aacaagcacc atgtcggggc ggccatcagg accaagactg
     4501 gggagatcat ctctgctgtc cacattgagg cctacattgg cagggtcact gtctgtgctg
     4561 aagccattgc cattgggtct gctgtgagca acgggcagaa ggactttgac accattgtgg
     4621 ctgtcaggca cccctactct gatgaggtgg acagatccat cagggtggtc agcccctgtg
     4681 gcatgtgcag agagctcatc tctgactatg ctcctgactg ctttgtgctc attgagatga
     4741 atggcaagct ggtcaaaacc accattgagg aactcatccc cctcaagtac accaggaact
     4801 aaacctgaat t
//
