LOCUS       sequence-347946-        4861 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express mCerulean3 fused to the N-terminus of the Bcl-2 family 
            chimera protein, Bcl-2-Cb5: Bcl-2 with its membrane binding region 
            swapped for Cb5 (ER targeting sequence)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4861)
  TITLE     David Andrews Lab plasmids
REFERENCE   2  (bases 1 to 4861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCerulean3-Bcl2-cb5-s2193
FEATURES             Location/Qualifiers
     source          1..4861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    33..154
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(44..63)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     98..117
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      342..929
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     830..849
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        1051..1242
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(1074..1094)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     regulatory      2364..2373
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2370..2372,2373..2375,2376..3086)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2415..2436)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2676..2695)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3023..3044
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3886..4330
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     promoter        4364..4410
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        4402..4420
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cttcgaactc gaggctagct ggccagacat gataagatac attgatgagt ttggacaaac
       61 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
      121 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
      181 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
      241 tggtatggaa atgttaatta actagccatg accaaaatcc cttaacgtga gttttcgttc
      301 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
      361 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
      421 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
      481 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
      541 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
      601 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
      661 acgggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
      721 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
      781 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
      841 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
      901 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
      961 tggccttttg ctggcctttt gctcacatgt tcttaattaa cctgcagggc ctgaaataac
     1021 ctctgaaaga ggaacttggt taggtacctt ctgaggctga aagaaccagc tgtggaatgt
     1081 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     1141 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     1201 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccactagttc cgccagagcg
     1261 cgcgagggcc tccagcggcc gcccctcccc cacagcaggg gcggggtccc gcgcccaccg
     1321 gaaggagcgg gctcggggcg ggcggcgctg attggccggg gcgggcctga cgccgacgcg
     1381 gctataagag accacaagcg acccgcaggg ccagacgttc ttcgccgaag cttgccgtca
     1441 gaacgcaggt gaggggcggg tgtggcttcc gcgggccgcc gagctggagg tcctgctccg
     1501 agcgggccgg gccccgctgt cgtcggcggg gattagctgc gagcattccc gcttcgagtt
     1561 gcgggcggcg cgggaggcag agtgcgaggc ctagcggcaa ccccgtagcc tcgcctcgtg
     1621 tccggcttga ggcctagcgt ggtgtccgcg ccgccgccgc gtgctactcc ggccgcactc
     1681 tggtcttttt tttttttgtt gttgttgccc tgctgccttc gattgccgtt cagcaatagg
     1741 ggctaacaaa gggagggtgc ggggcttgct cgcccggagc ccggagaggt catggttggg
     1801 gaggaatgga gggacaggag tggcggctgg ggcccgcccg ccttcggagc acatgtccga
     1861 cgccacctgg atggggcgag gcctggggtt tttcccgaag caaccaggct ggggttagcg
     1921 tgccgaggcc atgtggcccc agcacccggc acgatctggc ttggcggcgc cgcgttgccc
     1981 tgcctcccta actagggtga ggccatcccg tccggcacca gttgcgtgcg tggaaagatg
     2041 gccgctcccg ggccctgttg caaggagctc aaaatggagg acgcggcagc ccggtggagc
     2101 gggcgggtga gtcacccaca caaaggaaga gggcctggtc cctcaccggc tgctgcttcc
     2161 tgtgaccccg tggtcctatc ggccgcaata gtcacctcgg gcttttgagc acggctagtc
     2221 gcggcggggg gaggggatgt aatggcgttg gagtttgttc acatttggtg ggtggagact
     2281 agtcaggcca gcctggcgct ggaagtcatt tttggaattt gtccccttga gttttgagcg
     2341 gagctaattc aaagcaaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg
     2401 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     2461 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     2521 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgagctgg ggcgtgcagt
     2581 gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     2641 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     2701 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     2761 tcaaggagga cggcaacatc ctggggcaca agctggagta caacgccatc cacggcaacg
     2821 tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcggc ctcaactgca
     2881 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     2941 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccaag ctgagcaaag
     3001 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     3061 ctctcggcat ggacgagctg tacaagtccg gactcagatc cacgcgtatg gcgcacgctg
     3121 ggagaacagg gtacgataac cgggagatag tgatgaagta catccattat aagctgtcgc
     3181 agaggggcta cgagtgggat gcgggagatg tgggcgccgc gcccccgggg gccgcccccg
     3241 caccgggcat cttctcctcc cagcccgggc acacgcccca tccagccgca tcccgggacc
     3301 cggtcgccag gacctcgcca ctgcagaccc cggctgcccc cggcgccgcc gcggggcctg
     3361 cgctcagccc ggtgccacct gtggtccacc tgaccctccg ccaggccggc gacgacttct
     3421 cccgccgcta ccgccgcgac ttcgccgaga tgtccagcca gctgcacctg acgcccttca
     3481 ccgcgcgggg acgctttgcc acggtggtgg aggagctctt cagggacggg gtgaactggg
     3541 ggaggattgt ggccttcttt gagttcggtg gggtcatatg tgtggagagc gtcaaccggg
     3601 agatgtcgcc cctggtggac aacatcgccc tgtggatgac tgagtacctg aaccggcacc
     3661 tgcacacctg gatccaggat aacggaggct gggatgcctt tgtggagctc tacggcccca
     3721 gcatgcggcc tctgtttgat ttctcctggc tgtctcttaa gatcactacc gttgaatcga
     3781 actctagttg gtggactaac tgggttatcc ctgcgatctc tgctctggtt gtagcgctga
     3841 tgtaccgtct gtacatggct gaagattaat gaggtcgacc ctaggagcag gtttccccaa
     3901 tgacacaaaa cgtgcaactt gaaactccgc ctggtctttc caggtctaga ggggtaacac
     3961 tttgtactgc gtttggctcc acgctcgatc cactggcgag tgttagtaac agcactgttg
     4021 cttcgtagcg gagcatgacg gccgtgggaa ctcctccttg gtaacaagga cccacggggc
     4081 caaaagccac gcccacacgg gcccgtcatg tgtgcaaccc cagcacggcg actttactgc
     4141 gaaacccact ttaaagtgac attgaaactg gtacccacac actggtgaca ggctaaggat
     4201 gcccttcagg taccccgagg taacacgcga cactcgggat ctgagaaggg gactggggct
     4261 tctataaaag cgctcggttt aaaaagcttc tatgcctgaa taggtgaccg gaggtcggca
     4321 cctttccttt gcaattactg accctatgaa tacaactgac tgtttgacaa ttaatcatcg
     4381 gcatagtata tcggcatagt ataatacgac tcactatagg agggccacca tgaagacctt
     4441 caacatctct cagcaggatc tggagctggt ggaggtcgcc actgagaaga tcaccatgct
     4501 ctatgaggac aacaagcacc atgtcggggc ggccatcagg accaagactg gggagatcat
     4561 ctctgctgtc cacattgagg cctacattgg cagggtcact gtctgtgctg aagccattgc
     4621 cattgggtct gctgtgagca acgggcagaa ggactttgac accattgtgg ctgtcaggca
     4681 cccctactct gatgaggtgg acagatccat cagggtggtc agcccctgtg gcatgtgcag
     4741 agagctcatc tctgactatg ctcctgactg ctttgtgctc attgagatga atggcaagct
     4801 ggtcaaaacc accattgagg aactcatccc cctcaagtac accaggaact aaacctgaat
     4861 t
//
