LOCUS       sequence-34795-e         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Koushik SV, Blank PS, Vogel SS
  TITLE     Anomalous surplus energy transfer observed with multiple FRET 
            acceptors.
  JOURNAL   PLoS One. 2009 Nov 25;4(11):e8031. doi: 
            10.1371/journal.pone.0008031.
  PUBMED    19946626
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-11-25"; volume: "4(11):e8031. doi"; pages: " 
            10.1371/journal.pone.0008031"
COMMENT     Sequence Label: VCVV
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(59..769,770..772))
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(101..122)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     450..469
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     709..730
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 acataactca caatgtggta tggctgatta tgatcagtta tctagatccg gtggatccct
       61 tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca
      121 tgtgatcgcg cttctcgttg gggtctttgc tcagcttgga ctggtagctc aggtagtggt
      181 tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga
      241 gctgcacgcc gccgtcctcg atgttgtggc ggatcttgaa gttggccttg atgccgttct
      301 tctgcttgtc ggcggtgata tagacgttgt ggctgttgta gttgtactcc agcttgtgcc
      361 ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg
      421 tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga
      481 tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca
      541 tgtggtcggg gtagcgggcg aagcactgca ggccgtagcc gagggtggtc acgagggtgg
      601 gccagggcac gggcagcttg ccggtggtgc agatgagctt cagggtcagc ttgccgtagg
      661 tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca
      721 gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc acccgtcgac
      781 tgcagaattc c
//
