LOCUS       sequence-34798-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Koushik SV, Blank PS, Vogel SS
  TITLE     Anomalous surplus energy transfer observed with multiple FRET 
            acceptors.
  JOURNAL   PLoS One. 2009 Nov 25;4(11):e8031. doi: 
            10.1371/journal.pone.0008031.
  PUBMED    19946626
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-11-25"; volume: "4(11):e8031. doi"; pages: " 
            10.1371/journal.pone.0008031"
COMMENT     Sequence Label: ACA
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      44..53
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(50..52,53..55,56..766)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGCGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(356..375)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     703..724
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gagctggttt agtgaaccgt cagatccgct agcgctaccg gtcgccacca tggtgagcaa
       61 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
      121 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
      181 cctgaagctc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
      241 cctcggctgc ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt
      301 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
      361 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
      421 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
      481 caactacaac agccacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc
      541 caacttcaag atccgccaca acatcgagga cggcggcgtg cagctcgccg accactacca
      601 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta
      661 ccagtccaag ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
      721 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtccg gactcagatc
      781 tgtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
      841 cgacgtaaac ggccacaagt tcagcgtgtc
//
