LOCUS       sequence-34799-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Koushik SV, Blank PS, Vogel SS
  TITLE     Anomalous surplus energy transfer observed with multiple FRET 
            acceptors.
  JOURNAL   PLoS One. 2009 Nov 25;4(11):e8031. doi: 
            10.1371/journal.pone.0008031.
  PUBMED    19946626
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-11-25"; volume: "4(11):e8031. doi"; pages: " 
            10.1371/journal.pone.0008031"
COMMENT     Sequence Label: ACV
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(56..307)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(98..119)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     447..466
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     706..727
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 aactctacaa tgtggtatgg ctgattatga tcagttatct agatccggtg gatcccttgt
       61 acagctcgtc catgccgaga gtgatcccgg cggcggtcac gaactccagc aggaccatgt
      121 gatcgcgctt ctcgttgggg tctttgctca gcttggactg gtagctcagg tagtggttgt
      181 cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct
      241 gcacgccgcc gtcctcgatg ttgtggcgga tcttgaagtt ggccttgatg ccgttcttct
      301 gcttgtcggc ggtgatatag acgttgtggc tgttgtagtt gtactccagc ttgtgcccca
      361 ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc gatgcggttc accagggtgt
      421 cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg
      481 tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt
      541 ggtcggggta gcgggcgaag cactgcaggc cgtagccgag ggtggtcacg agggtgggcc
      601 agggcacggg cagcttgccg gtggtgcaga tgagcttcag ggtcagcttg ccgtaggtgg
      661 catcgccctc gccctcgccg gacacgctga acttgtggcc gtttacgtcg ccgtccagct
      721 cgaccaggat gggcaccacc cccggtgaac agctcctcgc ccttgctcac ccgtcgactg
      781 cagaattcct tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactca
//
