LOCUS       sequence-348003-        9456 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of Mma PylRS with nuclear export sequence for amber 
            suppression in mammalian cells; transient or piggy bac mediated 
            integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9456)
  AUTHORS   Elsaesser Lab
  TITLE     Elsaesser plasmids 2021
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9456)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAS_MmaPylT_EF1_NES-MmaPylRS WT
FEATURES             Location/Qualifiers
     source          1..9456
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        2727..2938
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2951..3219
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      3251..3260
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3260..3283
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3293..3319
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     misc_feature    join(4706..5280,5281..5283,5284..5292)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4873..4890)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5100..5119
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5305..5904
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5305..5324)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5801..5821
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(5964..5984)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    6026..6147
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6063..6082)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6117..6136
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   6910..6944
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7435..7451)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7435..7451)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7448..7470)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7459..7475
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7483..7489,7490..7507,7508..7513))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7528..7549
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7666..7683)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7837..8425)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7917..7936)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8596..9387,9388..9456))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9219..9238
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccaact agtgaaaaac cgcacttgtc
     1381 cggaaacccc gggaatctaa cccggctgaa cggatttaga gtccgttcga tctacatgat
     1441 caggtttccg aggtacccag gcggcgcaca agctatataa acctgaagga aatctcaact
     1501 ttacacttag gtcaagttac ttatcgtact agagcttcag caggaaattt aactaaaatc
     1561 taatttaacc agcatagcaa atatcattta ttcccaaaat gctaaagttt gagataaacg
     1621 gacttgattt ccggctgttt tgacactatc cagaatgcct tgcagatggg tggggcatgc
     1681 taaatactgc aggaaaaacc gcacttgtcc ggaaaccccg ggaatctaac ccggctgaac
     1741 ggatttagag tccgttcgat ctacatgatc aggtttccga ggtacccagg cggcgcacaa
     1801 gctatataaa cctgaaggaa atctcaactt tacacttagg tcaagttact tatcgtacta
     1861 gagcttcagc aggaaattta actaaaatct aatttaacca gcatagcaaa tatcatttat
     1921 tcccaaaatg ctaaagtttg agataaacgg acttgatttc cggctgtttt gacactatcc
     1981 agaatgcctt gcagatgggt ggggcatgct aaatactgca ggaaaaaccg cacttgtccg
     2041 gaaaccccgg gaatctaacc cggctgaacg gatttagagt ccgttcgatc tacatgatca
     2101 ggtttccgag gtacccaggc ggcgcacaag ctatataaac ctgaaggaaa tctcaacttt
     2161 acacttaggt caagttactt atcgtactag agcttcagca ggaaatttaa ctaaaatcta
     2221 atttaaccag catagcaaat atcatttatt cccaaaatgc taaagtttga gataaacgga
     2281 cttgatttcc ggctgttttg acactatcca gaatgccttg cagatgggtg gggcatgcta
     2341 aatactgcag gaaaaaccgc acttgtccgg aaaccccggg aatctaaccc ggctgaacgg
     2401 atttagagtc cgttcgatct acatgatcag gtttccgagg tacccaggcg gcgcacaagc
     2461 tatataaacc tgaaggaaat ctcaacttta cacttaggtc aagttactta tcgtactaga
     2521 gcttcagcag gaaatttaac taaaatctaa tttaaccagc atagcaaata tcatttattc
     2581 ccaaaatgct aaagtttgag ataaacggac ttgatttccg gctgttttga cactatccag
     2641 aatgccttgc agatgggtgg ggcatgctaa atactgcaga actagtgctc tagaaaggat
     2701 ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
     2761 gttgggggga ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg
     2821 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     2881 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct
     2941 gaagcttcga ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca
     3001 tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc
     3061 cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg
     3121 gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta
     3181 cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac
     3241 tctagagcta gccaccatgg actacaagga cgacgacgac aagccgctgc agctgccgcc
     3301 gctggaacgc ctgaccctgg atatggacaa gaagcccctg aacaccctga tcagcgccac
     3361 aggactgtgg atgtccagaa ccggcaccat ccacaagatc aagcaccacg aggtgtcccg
     3421 gtccaaaatc tacatcgaga tggcctgcgg cgatcacctg gtcgtcaaca acagcagaag
     3481 cagccggaca gccagagccc tgcggcacca caagtacaga aagacctgca agcggtgcag
     3541 agtgtccgac gaggacctga acaagttcct gaccaaggcc aacgaggacc agaccagcgt
     3601 gaaagtgaag gtggtgtccg cccccacccg gaccaagaaa gccatgccca agagcgtggc
     3661 cagagccccc aagcccctgg aaaacaccga agccgctcag gcccagccca gcggcagcaa
     3721 gttcagcccc gccatccccg tgtctaccca ggaaagcgtc agcgtccccg ccagcgtgtc
     3781 caccagcatc tctagcatct caaccggcgc cacagcttct gccctggtca agggcaacac
     3841 caaccccatc accagcatgt ctgcccctgt gcaggcctct gccccagccc tgaccaagtc
     3901 ccagaccgac cggctggaag tgctcctgaa ccccaaggac gagatcagcc tgaacagcgg
     3961 caagcccttc cgggagctgg aaagcgagct gctgagccgg cggaagaagg acctccagca
     4021 aatctacgcc gaggaacggg agaactacct gggcaagctg gaaagagaga tcacccggtt
     4081 cttcgtggac cggggcttcc tggaaatcaa gagccccatc ctgatccccc tggagtacat
     4141 cgagcggatg ggcatcgaca acgacaccga gctgagcaag cagattttcc gggtggacaa
     4201 gaacttctgc ctgcggccca tgctggcccc caacctgtac aactacctgc ggaaactgga
     4261 tcgcgctctg cccgacccca tcaagatttt cgagatcggc ccctgctacc ggaaagagag
     4321 cgacggcaaa gagcacctgg aagagtttac aatgctgaac ttttgccaga tgggcagcgg
     4381 ctgcaccaga gagaacctgg aatccatcat caccgacttt ctgaaccacc tggggatcga
     4441 cttcaagatc gtgggcgaca gctgcatggt gtacggcgac accctggacg tgatgcacgg
     4501 cgacctggaa ctgtctagcg ccgtcgtggg acccatccct ctggaccggg agtggggcat
     4561 cgataagccc tggatcggag ccggcttcgg cctggaacgg ctgctgaaag tcaagcacga
     4621 ctttaagaac atcaagcggg ctgccagaag cgagagctac tacaacggca tcagcaccaa
     4681 cctgtgatga ggatccgcgg ccgcgcccct ctccctcccc cccccctaac gttactggcc
     4741 gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc
     4801 cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta
     4861 ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag
     4921 ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga
     4981 accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg
     5041 caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat
     5101 ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta
     5161 tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa
     5221 aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat
     5281 atggccacaa ccatggcgtc cggaatgacc gagtacaagc ccacggtgcg cctcgccacc
     5341 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     5401 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     5461 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     5521 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     5581 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     5641 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     5701 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     5761 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     5821 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     5881 atgacccgca agcccggtgc ctgagtcgac aatcaacctc tggattacaa aatttgtgaa
     5941 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     6001 atgcctttgt atcatgcgtt aactaaactt gtttattgca gcttataatg gttacaaata
     6061 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     6121 tttgtccaaa ctcatcaatg tatcttatca tgtctggaat tgactcaaat gatgtcaatt
     6181 agtctatcag aagctcatct ggtctccctt ccgggggaca agacatccct gtttaatatt
     6241 taaacagcag tgttcccaaa ctgggttctt atatcccttg ctctggtcaa ccaggttgca
     6301 gggtttcctg tcctcacagg aacgaagtcc ctaaagaaac agtggcagcc aggtttagcc
     6361 ccggaattga ctggattcct tttttagggc ccattggtat ggctttttcc ccgtatcccc
     6421 ccaggtgtct gcaggctcaa agagcagcga gaagcgttca gaggaaagcg atcccgtgcc
     6481 accttccccg tgcccgggct gtccccgcac gctgccggct cggggatgcg gggggagcgc
     6541 cggaccggag cggagccccg ggcggctcgc tgctgccccc tagcggggga gggacgtaat
     6601 tacatccctg ggggctttgg gggggggctg tccctgatat ctataacaag aaaatatata
     6661 tataataagt tatcacgtaa gtagaacatg aaataacaat ataattatcg tatgagttaa
     6721 atcttaaaag tcacgtaaaa gataatcatg cgtcattttg actcacgcgg tcgttatagt
     6781 tcaaaatcag tgacacttac cgcattgaca agcacgcctc acgggagctc caagcggcga
     6841 ctgagatgtc ctaaatgcac agcgacggat tcgcgctatt tagaaagaga gagcaatatt
     6901 tcaagaatgc atgcgtcaat tttacgcaga ctatctttct agggttaatc tagctgcatc
     6961 aggatcatat cgtcgggtct tttttccggc tcagtcatcg cccaagctgg cgctatctgg
     7021 gcatcgggga ggaagaagcc cgtgcctttt cccgcgaggt tgaagcggca tggaaagagt
     7081 ttgccgagga tgactgctgc tgcattgacg ttgagcgaaa acgcacgttt accatgatga
     7141 ttcgggaagg tgtggccatg cacgccttta acggtgaact gttcgttcag gccacctggg
     7201 ataccagttc gtcgcggctt ttccggacac agttccggat ggtcagcccg aagcgcatca
     7261 gcaacccgaa caataccggc gacagccgga actgccgtgc cggtgtgcag attaatgaca
     7321 gcggtgcggc gctgggatat tacgtcagcg aggacgggta tcctggctgg atgccgcaga
     7381 aatggacatg gataccccgt gagttacccg gcgggcgcgc ttggcgtaat catggtcata
     7441 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     7501 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     7561 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     7621 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     7681 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     7741 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     7801 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     7861 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     7921 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     7981 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     8041 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     8101 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     8161 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     8221 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     8281 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     8341 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     8401 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     8461 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     8521 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     8581 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     8641 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     8701 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     8761 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     8821 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     8881 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     8941 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     9001 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     9061 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     9121 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     9181 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     9241 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9301 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9361 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     9421 gggaataagg gcgacacgga aatgttgaat actcat
//
