LOCUS       sequence-348020-        2840 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  miR218 nt5 point mutation in SLIT3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Liu J, Li Y, Lyu L, Xiao L, Memon AA, Yu X, Halim A, Patel S, Osman 
            A, Yin W, Jiang J, Naini S, Lim K, Zhang A, Williams JD, Koester R, 
            Qi KZ, Fucci QA, Ding L, Chang S, Patel A, Mori Y, Chaudhari A, Bao 
            A, Liu J, Lu TS, Siedlecki A
  TITLE     Integrin alpha5 Is Regulated by miR-218-5p in Endothelial Progenitor
            Cells.
  JOURNAL   J Am Soc Nephrol. 2022 Mar;33(3):565-582. doi: 
            10.1681/ASN.2021020140. Epub 2022 Jan 28.
  PUBMED    35091451
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1681/ASN.2021020140"; journalName: "J Am Soc Nephrol"; date: 
            "2022-03"; volume: "33"; issue: "3"; pages: "565-582"
COMMENT     Sequence Label: pMIR218-2_nt5G>U
FEATURES             Location/Qualifiers
     source          1..2840
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(155..743)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(235..254)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(914..1729)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRFAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1637..1656
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(1730..1834)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1902..1920
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1958..1980)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2080..2099
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2293..2315
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2307..2324
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2308..2324
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2593..2609)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2593..2609)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2606..2628)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2617..2633
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2641..2647,2648..2665,2666..2671))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2686..2707
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(join(2824..2840,1))
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
       61 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
      121 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      181 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      241 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      301 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
      361 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      421 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      481 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
      541 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
      601 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      661 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
      721 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
      781 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
      841 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
      901 cttggtctga cagttagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat
      961 caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac
     1021 cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa
     1081 catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac
     1141 catgagtgac gactgaatcc ggtgagaatg gcaaaagttt atgcatttct ttccagactt
     1201 gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat
     1261 tcattcgtga ttgcgcctga gcgaaacgaa atacgcgatc gctgttaaaa ggacaattac
     1321 aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac
     1381 ctgaatcagg atattcttct aatacctgga atgctgtttt cccagggatc gcagtggtga
     1441 gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt
     1501 ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc
     1561 catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac
     1621 ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg
     1681 aatttaatcg cggcctagag caagacgttt cccgttgaat atggctcata ctcttccttt
     1741 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     1801 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     1861 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     1921 ccttttgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     1981 agactgtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     2041 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
     2101 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     2161 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     2221 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
     2281 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattg acgcgtattg
     2341 ggatgaaccc agaacgtctg tagcttttct cctccttccc tccattttcc tcttggtctt
     2401 acctttggcc tagtggttgg tgtagtgata atgtagcgag attttctgtt gttcttgatc
     2461 taaccatgtg gttgcgaggt atgagtaaaa catggttccg tcaagcacca tggaacgtca
     2521 cgcagctttc tacagcatga caagctgctg agggttccat cccaatggcg cgccgagctt
     2581 ggctcgagca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     2641 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     2701 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     2761 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctgttccgc
     2821 ttcctcgctc actgactcgc
//
