LOCUS       sequence-348027-       11775 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast plasmid encoding the third-generation CRISPR base editor BE3 
            (Komor et al., Nature 2016) under a galactose-inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11775)
  AUTHORS   Schubert OT, Bloom JS, Sadhu MJ, Kruglyak L
  TITLE     Genome-wide base editor screen identifies regulators of protein 
            abundance in yeast.
  JOURNAL   Elife. 2022 Nov 3;11. pii: 79525. doi: 10.7554/eLife.79525.
  PUBMED    36326816
REFERENCE   2  (bases 1 to 11775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-11-3"; volume: "11. pii: 79525. doi"; pages: " 
            10.7554/eLife.79525"
COMMENT     Sequence Label: pGal-BE3
FEATURES             Location/Qualifiers
     source          1..11775
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        519..623
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(624..692,693..1484)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(842..861)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1655..2243
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2144..2163
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2397..2414
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2531..2552
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2567..2572,2573..2590,2591..2597)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2605..2621
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2610..2632
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2629..2645
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2629..2645
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2664..2684
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2666..2684
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     3093..3116
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     3137..3153
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3137..3153
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             3167..3853
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             join(3902..7300,7301..7303,7304..7900,7901,7902..7904,
                     7905..7906,7907..7909,7910..8002)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(3902..3925,3926..3928,3929..8002)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             8015..8263
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             8276..8296
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      8312..8559
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(8328..8346)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(8577..8596)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8578..8596)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8606..8623)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8606..8622)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8615..8637)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8763..9218
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8850..8869)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9060..9081
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9519..9926
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             9927..11021
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(11515..11534)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11634..11656
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(11694..11712)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    join(11754..11775,1..482)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
ORIGIN
        1 cgcgcctcgt atcttttaat gatggaataa tttgggaatt tactctgtgt ttatttattt
       61 ttatgttttg tatttggatt ttagaaagta aataaagaag gtagaagagt tacggaatga
      121 agaaaaaaaa ataaacaaag gtttaaaaaa tttcaacaaa aagcgtactt tacatatata
      181 tttattagac aagaaaagca gattaaatag atatacattc gattaacgat aagtaaaatg
      241 taaaatcaca ggattttcgt gtgtggtctt ctacacagac aagatgaaac aattcggcat
      301 taatacctga gagcaggaag agcaagataa aaggtagtat ttgttggcga tccccctaga
      361 gtcttttaca tcttcggaaa acaaaaacta ttttttcttt aatttctttt tttactttct
      421 atttttaatt tatatattta tattaaaaaa tttaaattat aattattttt atagcacgtg
      481 atgaaaagga cccaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
      541 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
      601 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
      661 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
      721 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
      781 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
      841 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
      901 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
      961 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     1021 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     1081 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     1141 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     1201 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     1261 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     1321 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     1381 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     1441 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     1501 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     1561 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     1621 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     1681 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     1741 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     1801 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     1861 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     1921 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     1981 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     2041 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     2101 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     2161 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     2221 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     2281 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     2341 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     2401 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     2461 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     2521 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     2581 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     2641 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
     2701 tcgtgacagc cctccgaagg aagactctcc tccgtgcgtc ctcgtcttca ccggtcgcgt
     2761 tcctgaaacg cagatgtgcc tcgcgccgca ctgctccgaa caataaagat tctacaatac
     2821 tagcttttat ggttatgaag aggaaaaatt ggcagtaacc tggccccaca aaccttcaaa
     2881 tgaacgaatc aaattaacaa ccataggatg ataatgcgat tagtttttta gccttatttc
     2941 tggggtaatt aatcagcgaa gcgatgattt ttgatctatt aacggatata taaatgcaaa
     3001 aactgcataa ccactttaac taatactttc aacattttcg gtttgtatta cttcttattc
     3061 aaatgtaata aaagtatcaa caaaaaattg ttaatatacc tctatacttt aacgtcaagg
     3121 agaaaaaacc ccggattcta gaactagtgg atcccccggg aaaaaaatga gctcagagac
     3181 tggcccagtg gctgtggacc ccacattgag acggcggatc gagccccatg agtttgaggt
     3241 attcttcgat ccgagagagc tccgcaagga gacctgcctg ctttacgaaa ttaattgggg
     3301 gggccggcac tccatttggc gacatacatc acagaacact aacaagcacg tcgaagtcaa
     3361 cttcatcgag aagttcacga cagaaagata tttctgtccg aacacaaggt gcagcattac
     3421 ctggtttctc agctggagcc catgcggcga atgtagtagg gccatcactg aattcctgtc
     3481 aaggtatccc cacgtcactc tgtttattta catcgcaagg ctgtaccacc acgctgaccc
     3541 ccgcaatcga caaggcctgc gggatttgat ctcttcaggt gtgactatcc aaattatgac
     3601 tgagcaggag tcaggatact gctggagaaa ctttgtgaat tatagcccga gtaatgaagc
     3661 ccactggcct aggtatcccc atctgtgggt acgactgtac gttcttgaac tgtactgcat
     3721 catactgggc ctgcctcctt gtctcaacat tctgagaagg aagcagccac agctgacatt
     3781 ctttaccatc gctcttcagt cttgtcatta ccagcgactg cccccacaca ttctctgggc
     3841 caccgggttg aaaagcggca gcgagactcc cgggacctca gagtccgcca cacccgaaag
     3901 tgataaaaag tattctattg gtttagccat cggcactaat tccgttggat gggctgtcat
     3961 aaccgatgaa tacaaagtac cttcaaagaa atttaaggtg ttggggaaca cagaccgtca
     4021 ttcgattaaa aagaatctta tcggtgccct cctattcgat agtggcgaaa cggcagaggc
     4081 gactcgcctg aaacgaaccg ctcggagaag gtatacacgt cgcaagaacc gaatatgtta
     4141 cttacaagaa atttttagca atgagatggc caaagttgac gattctttct ttcaccgttt
     4201 ggaagagtcc ttccttgtcg aagaggacaa gaaacatgaa cggcacccca tctttggaaa
     4261 catagtagat gaggtggcat atcatgaaaa gtacccaacg atttatcacc tcagaaaaaa
     4321 gctagttgac tcaactgata aagcggacct gaggttaatc tacttggctc ttgcccatat
     4381 gataaagttc cgtgggcact ttctcattga gggtgatcta aatccggaca actcggatgt
     4441 cgacaaactg ttcatccagt tagtacaaac ctataatcag ttgtttgaag agaaccctat
     4501 aaatgcaagt ggcgtggatg cgaaggctat tcttagcgcc cgcctctcta aatcccgacg
     4561 gctagaaaac ctgatcgcac aattacccgg agagaagaaa aatgggttgt tcggtaacct
     4621 tatagcgctc tcactaggcc tgacaccaaa ttttaagtcg aacttcgact tagctgaaga
     4681 tgccaaattg cagcttagta aggacacgta cgatgacgat ctcgacaatc tactggcaca
     4741 aattggagat cagtatgcgg acttattttt ggctgccaaa aaccttagcg atgcaatcct
     4801 cctatctgac atactgagag ttaatactga gattaccaag gcgccgttat ccgcttcaat
     4861 gatcaaaagg tacgatgaac atcaccaaga cttgacactt ctcaaggccc tagtccgtca
     4921 gcaactgcct gagaaatata aggaaatatt ctttgatcag tcgaaaaacg ggtacgcagg
     4981 ttatattgac ggcggagcga gtcaagagga attctacaag tttatcaaac ccatattaga
     5041 gaagatggat gggacggaag agttgcttgt aaaactcaat cgcgaagatc tactgcgaaa
     5101 gcagcggact ttcgacaacg gtagcattcc acatcaaatc cacttaggcg aattgcatgc
     5161 tatacttaga aggcaggagg atttttatcc gttcctcaaa gacaatcgtg aaaagattga
     5221 gaaaatccta acctttcgca taccttacta tgtgggaccc ctggcccgag ggaactctcg
     5281 gttcgcatgg atgacaagaa agtccgaaga aacgattact ccatggaatt ttgaggaagt
     5341 tgtcgataaa ggtgcgtcag ctcaatcgtt catcgagagg atgaccaact ttgacaagaa
     5401 tttaccgaac gaaaaagtat tgcctaagca cagtttactt tacgagtatt tcacagtgta
     5461 caatgaactc acgaaagtta agtatgtcac tgagggcatg cgtaaacccg cctttctaag
     5521 cggagaacag aagaaagcaa tagtagatct gttattcaag accaaccgca aagtgacagt
     5581 taagcaattg aaagaggact actttaagaa aattgaatgc ttcgattctg tcgagatctc
     5641 cggggtagaa gatcgattta atgcgtcact tggtacgtat catgacctcc taaagataat
     5701 taaagataag gacttcctgg ataacgaaga gaatgaagat atcttagaag atatagtgtt
     5761 gactcttacc ctctttgaag atcgggaaat gattgaggaa agactaaaaa catacgctca
     5821 cctgttcgac gataaggtta tgaaacagtt aaagaggcgt cgctatacgg gctggggacg
     5881 attgtcgcgg aaacttatca acgggataag agacaagcaa agtggtaaaa ctattctcga
     5941 ttttctaaag agcgacggct tcgccaatag gaactttatg cagctgatcc atgatgactc
     6001 tttaaccttc aaagaggata tacaaaaggc acaggtttcc ggacaagggg actcattgca
     6061 cgaacatatt gcgaatcttg ctggttcgcc agccatcaaa aagggcatac tccagacagt
     6121 caaagtagtg gatgagctag ttaaggtcat gggacgtcac aaaccggaaa acattgtaat
     6181 cgagatggca cgcgaaaatc aaacgactca gaaggggcaa aaaaacagtc gagagcggat
     6241 gaagagaata gaagagggta ttaaagaact gggcagccag atcttaaagg agcatcctgt
     6301 ggaaaatacc caattgcaga acgagaaact ttacctctat tacctacaaa atggaaggga
     6361 catgtatgtt gatcaggaac tggacataaa ccgtttatct gattacgacg tcgatcacat
     6421 tgtaccccaa tcctttttga aggacgattc aatcgacaat aaagtgctta cacgctcgga
     6481 taagaaccga gggaaaagtg acaatgttcc aagcgaggaa gtcgtaaaga aaatgaagaa
     6541 ctattggcgg cagctcctaa atgcgaaact gataacgcaa agaaagttcg ataacttaac
     6601 taaagctgag aggggtggct tgtctgaact tgacaaggcc ggatttatta aacgtcagct
     6661 cgtggaaacc cgccaaatca caaagcatgt tgcacagata ctagattccc gaatgaatac
     6721 gaaatacgac gagaacgata agctgattcg ggaagtcaaa gtaatcactt taaagtcaaa
     6781 attggtgtcg gacttcagaa aggattttca attctataaa gttagggaga taaataacta
     6841 ccaccatgcg cacgacgctt atcttaatgc cgtcgtaggg accgcactca ttaagaaata
     6901 cccgaagcta gaaagtgagt ttgtgtatgg tgattacaaa gtttatgacg tccgtaagat
     6961 gatcgcgaaa agcgaacagg agataggcaa ggctacagcc aaatacttct tttattctaa
     7021 cattatgaat ttctttaaga cggaaatcac tctggcaaac ggagagatac gcaaacgacc
     7081 tttaattgaa accaatgggg agacaggtga aatcgtatgg gataagggcc gggacttcgc
     7141 gacggtgaga aaagttttgt ccatgcccca agtcaacata gtaaagaaaa ctgaggtgca
     7201 gaccggaggg ttttcaaagg aatcgattct tccaaaaagg aatagtgata agctcatcgc
     7261 tcgtaaaaag gactgggacc cgaaaaagta cggtggcttc gatagcccta cagttgccta
     7321 ttctgtccta gtagtggcaa aagttgagaa gggaaaatcc aagaaactga agtcagtcaa
     7381 agaattattg gggataacga ttatggagcg ctcgtctttt gaaaagaacc ccatcgactt
     7441 ccttgaggcg aaaggttaca aggaagtaaa aaaggatctc ataattaaac taccaaagta
     7501 tagtctgttt gagttagaaa atggccgaaa acggatgttg gctagcgccg gagagcttca
     7561 aaaggggaac gaactcgcac taccgtctaa atacgtgaat ttcctgtatt tagcgtccca
     7621 ttacgagaag ttgaaaggtt cacctgaaga taacgaacag aagcaacttt ttgttgagca
     7681 gcacaaacat tatctcgacg aaatcataga gcaaatttcg gaattcagta agagagtcat
     7741 cctagctgat gccaatctgg acaaagtatt aagcgcatac aacaagcaca gggataaacc
     7801 catacgtgag caggcggaaa atattatcca tttgtttact cttaccaacc tcggcgctcc
     7861 agccgcattc aagtattttg acacaacgat agatcgcaaa cgatacactt ctaccaagga
     7921 ggtgctagac gcgacactga ttcaccaatc catcacggga ttatatgaaa ctcggataga
     7981 tttgtcacag cttgggggtg actctggtgg ttctactaat ctgtcagata ttattgaaaa
     8041 ggagaccggt aagcaactgg ttatccagga atccatcctc atgctcccag aggaggtgga
     8101 agaagtcatt gggaacaagc cggaaagcga tatactcgtg cacaccgcct acgacgagag
     8161 caccgacgag aatgtcatgc ttctgactag cgacgcccct gaatacaagc cttgggctct
     8221 ggtcatacag gatagcaacg gtgagaacaa gattaagatg ctctctggtg gttctcccaa
     8281 gaagaagagg aaagtctaat ctcttctcga gtcatgtaat tagttatgtc acgcttacat
     8341 tcacgccctc cccccacatc cgctctaacc gaaaaggaag gagttagaca acctgaagtc
     8401 taggtcccta tttatttttt tatagttatg ttagtattaa gaacgttatt tatatttcaa
     8461 atttttcttt tttttctgta cagacgcgtg tacgcatgta acattatact gaaaaccttg
     8521 cttgagaagg ttttgggacg ctcgaaggct ttaatttgcg gccggtaccc aattcgccct
     8581 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     8641 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     8701 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     8761 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     8821 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     8881 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     8941 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     9001 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     9061 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     9121 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     9181 taacgcgaat tttaacaaaa tattaacgct tacaatttcc tgatgcggta ttttctcctt
     9241 acgcatctgt gcggtatttc acaccgcata tcgaccggtc gaggagaact tctagtatat
     9301 ctacatacct aatattattg ccttattaaa aatggaatcc caacaattac atcaaaatcc
     9361 acattctctt caaaatcaat tgtcctgtac ttccttgttc atgtgtgttc aaaaacgtta
     9421 tatttatagg ataattatac tctatttctc aacaagtaat tggttgtttg gccgagcggt
     9481 ctaaggcgcc tgattcaaga aatatcttga ccgcagttaa ctgtgggaat actcaggtat
     9541 cgtaagatgc aagagttcga atctcttagc aaccattatt tttttcctca acataacgag
     9601 aacacacagg ggcgctatcg cacagaatca aattcgatga ctggaaattt tttgttaatt
     9661 tcagaggtcg cctgacgcat ataccttttt caactgaaaa attgggagaa aaaggaaagg
     9721 tgagagcgcc ggaaccggct tttcatatag aatagagaag cgttcatgac taaatgcttg
     9781 catcacaata cttgaagttg acaatattat ttaaggacct attgtttttt ccaataggtg
     9841 gttagcaatc gtcttacttt ctaacttttc ttacctttta catttcagca atatatatat
     9901 atatatttca aggatatacc attctaatgt ctgcccctaa gaagatcgtc gttttgccag
     9961 gtgaccacgt tggtcaagaa atcacagccg aagccattaa ggttcttaaa gctatttctg
    10021 atgttcgttc caatgtcaag ttcgatttcg aaaatcattt aattggtggt gctgctatcg
    10081 atgctacagg tgttccactt ccagatgagg cgctggaagc ctccaagaag gctgatgccg
    10141 ttttgttagg tgctgtgggt ggtcctaaat ggggtaccgg tagtgttaga cctgaacaag
    10201 gtttactaaa aatccgtaaa gaacttcaat tgtacgccaa cttaagacca tgtaactttg
    10261 catccgactc tcttttagac ttatctccaa tcaagccaca atttgctaaa ggtactgact
    10321 tcgttgttgt cagagaatta gtgggaggta tttactttgg taagagaaag gaagacgatg
    10381 gtgatggtgt cgcttgggat agtgaacaat acaccgttcc agaagtgcaa agaatcacaa
    10441 gaatggccgc tttcatggcc ctacaacatg agccaccatt gcctatttgg tccttggata
    10501 aagctaatgt tttggcctct tcaagattat ggagaaaaac tgtggaggaa accatcaaga
    10561 acgaattccc tacattgaag gttcaacatc aattgattga ttctgccgcc atgatcctag
    10621 ttaagaaccc aacccaccta aatggtatta taatcaccag caacatgttt ggtgatatca
    10681 tctccgatga agcctccgtt atcccaggtt ccttgggttt gttgccatct gcgtccttgg
    10741 cctctttgcc agacaagaac accgcatttg gtttgtacga accatgccac ggttctgctc
    10801 cagatttgcc aaagaataag gtcaacccta tcgccactat cttgtctgct gcaatgatgt
    10861 tgaaattgtc attgaacttg cctgaagaag gtaaggccat tgaagatgca gttaaaaagg
    10921 ttttggatgc aggtatcaga actggtgatt taggtggttc caacagtacc accgaagtcg
    10981 gtgatgctgt cgccgaagaa gttaagaaaa tccttgctta aaaagattct ctttttttat
    11041 gatatttgta cataaacttt ataaatgaaa ttcataatag aaacgacacg aaattacaaa
    11101 atggaatatg ttcatagggt agacgaaact atatacgcaa tctacataca tttatcaaga
    11161 aggagaaaaa ggaggatgta aaggaataca ggtaagcaaa ttgatactaa tggctcaacg
    11221 tgataaggaa aaagaattgc actttaacat taatattgac aaggaggagg gcaccacaca
    11281 aaaagttagg tgtaacagaa aatcatgaaa ctatgattcc taatttatat attggaggat
    11341 tttctctaaa aaaaaaaaaa tacaacaaat aaaaaacact caatgacctg accatttgat
    11401 ggagtttaag tcaatacctt cttgaaccat ttcccataat ggtgaaagtt ccctcaagaa
    11461 ttttactctg tcagaaacgg ccttaacgac gtagtcgata tggtgcactc tcagtacaat
    11521 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
    11581 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
    11641 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
    11701 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttagg acggatcgct
    11761 tgcctgtaac ttaca
//
