LOCUS       sequence-348040-       10827 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mma PylRS expression for amber suppression in mammalian cells; 
            transient or piggy bac mediated integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10827)
  AUTHORS   van Husen LS, Katsori AM, Meineke B, Tjernberg LO, Schedin-Weiss S, 
            Elsasser SJ
  TITLE     Engineered Human Induced Pluripotent Cells Enable Genetic Code 
            Expansion in Brain Organoids.
  JOURNAL   Chembiochem. 2021 Aug 25. doi: 10.1002/cbic.202100399.
  PUBMED    34431592
REFERENCE   2  (bases 1 to 10827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Chembiochem. 2021 Aug 25. doi: 10.1002/cbic.202100399."
COMMENT     Sequence Label: pAS_8xMmaPylT_EF1_FLAG-MmaPylRS
FEATURES             Location/Qualifiers
     source          1..10827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(1448..1696)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        complement(1798..2046)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        complement(2148..2396)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        complement(2498..2746)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        4116..4327
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             4340..4608
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      4661..4670
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4670..4693
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    join(6077..6651,6652..6654,6655..6663)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6244..6261)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6471..6490
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6676..7275
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6676..6695)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7172..7192
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(7335..7355)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    7397..7518
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7434..7453)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7488..7507
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   8281..8315
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8806..8822)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8806..8822)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8819..8841)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8830..8846
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8854..8860,8861..8878,8879..8884))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8899..8920
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9037..9054)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9208..9796)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9288..9307)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9967..10758,10759..10827))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10590..10609
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccacta ggaaaaaccg cacttgtccg
     1381 gaaaccccgg gaatctaacc cggctgaacg gatttagagt ccgttcgatc tacatgatca
     1441 ggtttccggt gtttcgtcct ttccacaaga tatataaagc caagaaatcg aaatactttc
     1501 aagttacggt aagcatatga tagtccattt taaaacataa ttttaaaact gcaaactacc
     1561 caagaaatta ttactttcta cgtcacgtat tttgtactaa tatctttgtg tttacagtca
     1621 aattaattct aattatctct ctaacagcct tgtatcgtat atgcaaatat gaaggaatca
     1681 tgggaaatag gccctcttcc tgcccaacta ggaaaaaccg cacttgtccg gaaaccccgg
     1741 gaatctaacc cggctgaacg gatttagagt ccgttcgatc tacatgatca ggtttccggt
     1801 gtttcgtcct ttccacaaga tatataaagc caagaaatcg aaatactttc aagttacggt
     1861 aagcatatga tagtccattt taaaacataa ttttaaaact gcaaactacc caagaaatta
     1921 ttactttcta cgtcacgtat tttgtactaa tatctttgtg tttacagtca aattaattct
     1981 aattatctct ctaacagcct tgtatcgtat atgcaaatat gaaggaatca tgggaaatag
     2041 gccctcttcc tgcccaacta ggaaaaaccg cacttgtccg gaaaccccgg gaatctaacc
     2101 cggctgaacg gatttagagt ccgttcgatc tacatgatca ggtttccggt gtttcgtcct
     2161 ttccacaaga tatataaagc caagaaatcg aaatactttc aagttacggt aagcatatga
     2221 tagtccattt taaaacataa ttttaaaact gcaaactacc caagaaatta ttactttcta
     2281 cgtcacgtat tttgtactaa tatctttgtg tttacagtca aattaattct aattatctct
     2341 ctaacagcct tgtatcgtat atgcaaatat gaaggaatca tgggaaatag gccctcttcc
     2401 tgcccaacta ggaaaaaccg cacttgtccg gaaaccccgg gaatctaacc cggctgaacg
     2461 gatttagagt ccgttcgatc tacatgatca ggtttccggt gtttcgtcct ttccacaaga
     2521 tatataaagc caagaaatcg aaatactttc aagttacggt aagcatatga tagtccattt
     2581 taaaacataa ttttaaaact gcaaactacc caagaaatta ttactttcta cgtcacgtat
     2641 tttgtactaa tatctttgtg tttacagtca aattaattct aattatctct ctaacagcct
     2701 tgtatcgtat atgcaaatat gaaggaatca tgggaaatag gccctcttcc tgcccaacta
     2761 gtgaaaaacc gcacttgtcc ggaaaccccg ggaatctaac ccggctgaac ggatttagag
     2821 tccgttcgat ctacatgatc aggtttccga ggtacccagg cggcgcacaa gctatataaa
     2881 cctgaaggaa atctcaactt tacacttagg tcaagttact tatcgtacta gagcttcagc
     2941 aggaaattta actaaaatct aatttaacca gcatagcaaa tatcatttat tcccaaaatg
     3001 ctaaagtttg agataaacgg acttgatttc cggctgtttt gacactatcc agaatgcctt
     3061 gcagatgggt ggggcatgct aaatactgca ggaaaaaccg cacttgtccg gaaaccccgg
     3121 gaatctaacc cggctgaacg gatttagagt ccgttcgatc tacatgatca ggtttccgag
     3181 gtacccaggc ggcgcacaag ctatataaac ctgaaggaaa tctcaacttt acacttaggt
     3241 caagttactt atcgtactag agcttcagca ggaaatttaa ctaaaatcta atttaaccag
     3301 catagcaaat atcatttatt cccaaaatgc taaagtttga gataaacgga cttgatttcc
     3361 ggctgttttg acactatcca gaatgccttg cagatgggtg gggcatgcta aatactgcag
     3421 gaaaaaccgc acttgtccgg aaaccccggg aatctaaccc ggctgaacgg atttagagtc
     3481 cgttcgatct acatgatcag gtttccgagg tacccaggcg gcgcacaagc tatataaacc
     3541 tgaaggaaat ctcaacttta cacttaggtc aagttactta tcgtactaga gcttcagcag
     3601 gaaatttaac taaaatctaa tttaaccagc atagcaaata tcatttattc ccaaaatgct
     3661 aaagtttgag ataaacggac ttgatttccg gctgttttga cactatccag aatgccttgc
     3721 agatgggtgg ggcatgctaa atactgcagg aaaaaccgca cttgtccgga aaccccggga
     3781 atctaacccg gctgaacgga tttagagtcc gttcgatcta catgatcagg tttccgaggt
     3841 acccaggcgg cgcacaagct atataaacct gaaggaaatc tcaactttac acttaggtca
     3901 agttacttat cgtactagag cttcagcagg aaatttaact aaaatctaat ttaaccagca
     3961 tagcaaatat catttattcc caaaatgcta aagtttgaga taaacggact tgatttccgg
     4021 ctgttttgac actatccaga atgccttgca gatgggtggg gcatgctaaa tactgcagaa
     4081 ctagtggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
     4141 ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg
     4201 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
     4261 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
     4321 aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg
     4381 aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg
     4441 aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc
     4501 gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc
     4561 tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc
     4621 ggcgcctact ctagagctag cgtttaaact taagaagctt gccaccatgg actacaagga
     4681 cgacgacgac aagatggaca agaagcccct gaacaccctg atcagcgcca caggactgtg
     4741 gatgtccaga accggcacca tccacaagat caagcaccac gaggtgtccc ggtccaaaat
     4801 ctacatcgag atggcctgcg gcgatcacct ggtcgtcaac aacagcagaa gcagccggac
     4861 agccagagcc ctgcggcacc acaagtacag aaagacctgc aagcggtgca gagtgtccga
     4921 cgaggacctg aacaagttcc tgaccaaggc caacgaggac cagaccagcg tgaaagtgaa
     4981 ggtggtgtcc gcccccaccc ggaccaagaa agccatgccc aagagcgtgg ccagagcccc
     5041 caagcccctg gaaaacaccg aagccgctca ggcccagccc agcggcagca agttcagccc
     5101 cgccatcccc gtgtctaccc aggaaagcgt cagcgtcccc gccagcgtgt ccaccagcat
     5161 ctctagcatc tcaaccggcg ccacagcttc tgccctggtc aagggcaaca ccaaccccat
     5221 caccagcatg tctgcccctg tgcaggcctc tgccccagcc ctgaccaagt cccagaccga
     5281 ccggctggaa gtgctcctga accccaagga cgagatcagc ctgaacagcg gcaagccctt
     5341 ccgggagctg gaaagcgagc tgctgagccg gcggaagaag gacctccagc aaatctacgc
     5401 cgaggaacgg gagaactacc tgggcaagct ggaaagagag atcacccggt tcttcgtgga
     5461 ccggggcttc ctggaaatca agagccccat cctgatcccc ctggagtaca tcgagcggat
     5521 gggcatcgac aacgacaccg agctgagcaa gcagattttc cgggtggaca agaacttctg
     5581 cctgcggccc atgctggccc ccaacctgta caactacctg cggaaactgg atcgcgctct
     5641 gcccgacccc atcaagattt tcgagatcgg cccctgctac cggaaagaga gcgacggcaa
     5701 agagcacctg gaagagttta caatgctgaa cttttgccag atgggcagcg gctgcaccag
     5761 agagaacctg gaatccatca tcaccgactt tctgaaccac ctggggatcg acttcaagat
     5821 cgtgggcgac agctgcatgg tgtacggcga caccctggac gtgatgcacg gcgacctgga
     5881 actgtctagc gccgtcgtgg gacccatccc tctggaccgg gagtggggca tcgataagcc
     5941 ctggatcgga gccggcttcg gcctggaacg gctgctgaaa gtcaagcacg actttaagaa
     6001 catcaagcgg gctgccagaa gcgagagcta ctacaacggc atcagcacca acctgtgatg
     6061 aggatccgcg gccgcgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct
     6121 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
     6181 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
     6241 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg
     6301 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac
     6361 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg
     6421 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct
     6481 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg
     6541 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag
     6601 gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca
     6661 accatggcgt ccggaatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     6721 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     6781 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     6841 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     6901 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     6961 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     7021 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     7081 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     7141 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     7201 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     7261 aagcccggtg cctaggtcga caatcaacct ctggattaca aaatttgtga aagattgact
     7321 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     7381 tatcatgcgt taactaaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     7441 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     7501 actcatcaat gtatcttatc atgtctggaa ttgactcaaa tgatgtcaat tagtctatca
     7561 gaagctcatc tggtctccct tccgggggac aagacatccc tgtttaatat ttaaacagca
     7621 gtgttcccaa actgggttct tatatccctt gctctggtca accaggttgc agggtttcct
     7681 gtcctcacag gaacgaagtc cctaaagaaa cagtggcagc caggtttagc cccggaattg
     7741 actggattcc ttttttaggg cccattggta tggctttttc cccgtatccc cccaggtgtc
     7801 tgcaggctca aagagcagcg agaagcgttc agaggaaagc gatcccgtgc caccttcccc
     7861 gtgcccgggc tgtccccgca cgctgccggc tcggggatgc ggggggagcg ccggaccgga
     7921 gcggagcccc gggcggctcg ctgctgcccc ctagcggggg agggacgtaa ttacatccct
     7981 gggggctttg ggggggggct gtccctgata tctataacaa gaaaatatat atataataag
     8041 ttatcacgta agtagaacat gaaataacaa tataattatc gtatgagtta aatcttaaaa
     8101 gtcacgtaaa agataatcat gcgtcatttt gactcacgcg gtcgttatag ttcaaaatca
     8161 gtgacactta ccgcattgac aagcacgcct cacgggagct ccaagcggcg actgagatgt
     8221 cctaaatgca cagcgacgga ttcgcgctat ttagaaagag agagcaatat ttcaagaatg
     8281 catgcgtcaa ttttacgcag actatctttc tagggttaat ctagctgcat caggatcata
     8341 tcgtcgggtc ttttttccgg ctcagtcatc gcccaagctg gcgctatctg ggcatcgggg
     8401 aggaagaagc ccgtgccttt tcccgcgagg ttgaagcggc atggaaagag tttgccgagg
     8461 atgactgctg ctgcattgac gttgagcgaa aacgcacgtt taccatgatg attcgggaag
     8521 gtgtggccat gcacgccttt aacggtgaac tgttcgttca ggccacctgg gataccagtt
     8581 cgtcgcggct tttccggaca cagttccgga tggtcagccc gaagcgcatc agcaacccga
     8641 acaataccgg cgacagccgg aactgccgtg ccggtgtgca gattaatgac agcggtgcgg
     8701 cgctgggata ttacgtcagc gaggacgggt atcctggctg gatgccgcag aaatggacat
     8761 ggataccccg tgagttaccc ggcgggcgcg cttggcgtaa tcatggtcat agctgtttcc
     8821 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     8881 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     8941 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     9001 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     9061 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     9121 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     9181 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     9241 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     9301 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     9361 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     9421 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     9481 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     9541 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     9601 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     9661 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     9721 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     9781 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     9841 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     9901 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     9961 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
    10021 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
    10081 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
    10141 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
    10201 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
    10261 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
    10321 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
    10381 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
    10441 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
    10501 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
    10561 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
    10621 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    10681 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    10741 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    10801 ggcgacacgg aaatgttgaa tactcat
//
