LOCUS       sequence-34805-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Koushik SV, Blank PS, Vogel SS
  TITLE     Anomalous surplus energy transfer observed with multiple FRET 
            acceptors.
  JOURNAL   PLoS One. 2009 Nov 25;4(11):e8031. doi: 
            10.1371/journal.pone.0008031.
  PUBMED    19946626
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-11-25"; volume: "4(11):e8031. doi"; pages: " 
            10.1371/journal.pone.0008031"
COMMENT     Sequence Label: ACVA
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(94..345)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(136..157)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     485..504
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     745..766
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 aactctacaa tgtggtatgg ctgattatga tcagttatct agatccggtg gatccactag
       61 taacggccgc cagtgtgctg gaattcgccc ttccttgtac agctcgtcca tgccgagagt
      121 gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc
      181 tttgctcagc ttggactggt agctcaggta gtggttgtcg ggcagcagca cggggccgtc
      241 gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgccgccgt cctcgatgtt
      301 gtggcggatc ttgaagttgg ccttgatgcc gttcttctgc ttgtcggcgg tgatatagac
      361 gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa
      421 gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc
      481 gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc
      541 gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc gggcgaagca
      601 ctgcaggccg cagccgaggg tggtcacgag ggtgggccag ggcacgggca gcttgccggt
      661 ggtgcagatg agcttcaggg tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga
      721 cacgctgaaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc
      781 cggtgaacag ctcctcgccc ttgctcaccc gtcgactgca gaattccttg tacagctcgt
      841 ccatgccgag a
//
