LOCUS       sequence-348058-       11042 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Small-molecule controlled base editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11042)
  AUTHORS   Berrios KN, Evitt NH, DeWeerd RA, Ren D, Luo M, Barka A, Wang T, 
            Bartman CR, Lan Y, Green AM, Shi J, Kohli RM
  TITLE     Controllable genome editing with split-engineered base editors.
  JOURNAL   Nat Chem Biol. 2021 Dec;17(12):1262-1270. doi: 
            10.1038/s41589-021-00880-w. Epub 2021 Oct 18.
  PUBMED    34663942
REFERENCE   2  (bases 1 to 11042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-021-00880-w"; journalName: "Nat Chem Biol"; date: 
            "2021-12"; volume: "17"; issue: "12"; pages: "1262-1270"
COMMENT     Sequence Label: Lenti-evoA1-seBEN_empty
FEATURES             Location/Qualifiers
     source          1..11042
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    211..763
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(355..372)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     582..601
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      778..787
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             784..1491
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(929..947)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1197..1216
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1501..1557
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1558..2352
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MLEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1612..1631)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2222..2241
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    2359..2947
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2412..2432)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2830..2841)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3019..3252
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3330..3451
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3367..3386)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3421..3440
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3491..3626
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3646..3665)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3647..3665)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3675..3692)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3675..3691)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3684..3706)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3833..4288
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3920..3939)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4130..4151
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4314..4418
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4419..4487,4488..5279)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4637..4656)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5450..6038
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5939..5958
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6192..6209
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6326..6347
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6362..6367,6368..6385,6386..6392)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6400..6416
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6405..6427
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6424..6440
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6424..6440
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6459..6479
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6461..6479
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6507..6733
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6734..6914
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6961..7086
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7579..7812
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     promoter        complement(9851..10099)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(9909..9928)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(10079..10099)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    10106..10223
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        10292..10503
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      10523..10532
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10532..10555
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      10556..10565
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10565..10594
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             10595..10642
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ttttagaacc gttgcacgct atgatggaga gaggtccaca gactctaaag gagacttcct
       61 tcaaccaagc ttatggaagg gacctaatgg aggctcaaga atggtgtaga aaatacatga
      121 aaagtggaaa tgtaaaggac cttacacaag cttgggatct ctactaccat gtttttagga
      181 gaatatctaa ataatgaccc ccccccccta acgttactgg ccgaagccgc ttggaataag
      241 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
      301 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
      361 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
      421 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
      481 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
      541 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
      601 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
      661 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
      721 accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggcccc caagcttgcc
      781 accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
      841 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
      901 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
      961 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
     1021 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
     1081 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
     1141 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
     1201 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
     1261 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
     1321 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
     1381 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     1441 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gggcagcggc
     1501 gccaccaact tcagcctgct gaagcaggcc ggcgacgtgg aggagaaccc cggccccatg
     1561 cttgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     1621 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     1681 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     1741 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     1801 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     1861 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     1921 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     1981 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     2041 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     2101 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     2161 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     2221 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     2281 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     2341 gagttcttct gagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     2401 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     2461 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     2521 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     2581 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     2641 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     2701 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     2761 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     2821 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     2881 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     2941 tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     3001 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     3061 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     3121 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     3181 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     3241 aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg
     3301 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     3361 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     3421 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     3481 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     3541 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     3601 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     3661 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     3721 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     3781 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     3841 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     3901 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     3961 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     4021 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     4081 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     4141 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     4201 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     4261 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     4321 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     4381 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     4441 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     4501 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     4561 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     4621 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     4681 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     4741 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     4801 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     4861 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     4921 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     4981 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     5041 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     5101 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     5161 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     5221 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     5281 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     5341 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     5401 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     5461 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     5521 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     5581 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     5641 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     5701 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     5761 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     5821 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     5881 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     5941 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6001 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6061 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     6121 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     6181 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     6241 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     6301 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     6361 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     6421 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     6481 aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa
     6541 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
     6601 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
     6661 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc
     6721 tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     6781 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     6841 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     6901 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     6961 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     7021 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     7081 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     7141 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     7201 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     7261 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     7321 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     7381 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     7441 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     7501 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     7561 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     7621 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     7681 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     7741 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     7801 tcaacagctc ctggggattt ggggttgctc tggaaaactc gccatttgtc tcaagatcta
     7861 gaattcaaaa aaagcaccga ctcggtgcca ctttgctgtt tccagcaaag ttgataacgg
     7921 actagcctta tttaaacttg ctatgctgtt tccagcatag ctcttaaaca gagacgtaca
     7981 aaaaagagca agaagctaaa aaagatttaa aaattatttt tagcgcagtt aatggaacag
     8041 gaactaaatt taccccaaaa atattacgtg aatcaggata taacgttatt gaggttgaag
     8101 agcatgcatt tgaagatgaa acatttaaaa atgttgtaaa tccaaatcca gaatttgatc
     8161 ctgcatgaaa aataccgctt gaatatggta ttaaacatga tgcagatatt attattatga
     8221 atgacccaga tgctgacaga tttggaatgg caataaaaca tgatggtcat tttgtaagat
     8281 tagatggaaa tcaaacagga ccaattttaa ttgattgaaa attatcaaat ctaaaacgct
     8341 taaatagcat tccaaaaaat ccggctctat attcaagttt tgtaacaagt gatttgggtg
     8401 atagaatcgc tcatgaaaaa tatggagtta atattgtaaa aactttaact ggatttaaat
     8461 gaatgggtag agaaattgct aaagaagaag ataacggatt aaattttgtt tttgcttatg
     8521 aagaaagtta tggatatgta attgatgact cagctagaga taaagatgga atacaagctt
     8581 ctatattaat agcagaggct gcttgatttt ataaaaaaca aaataaaaca ttagtagact
     8641 atttagaaga tttatttaaa gaaatgggtg catattacac tttcacttta aacttgaatt
     8701 ttaaaccaga agaaaagaaa ttaaaaattg aaccattaat gaaatcattg agagcaacac
     8761 ccttaactca aattgctgga cttaaagttg ttaatgttga agactacatc gatggaatgt
     8821 ataatatgcc aggacaagac ttactaaaat tttatttaga agataagtca tgatttgctg
     8881 ttcgcccaag tggaactgaa cctaaactaa aaatttattt tataggtgtt ggtgaatctg
     8941 ttcaaaacgc taaagttaaa gtagacgaaa ttattaaaga attaaaatta aaaatgaata
     9001 tataggagaa aaaatgaaac taaacaaata tatagatcac acattattaa aacaagatgc
     9061 tacgaaagct gaaattaaac aattatgtga tgaagcaatt gaatttgatt ttgcaacagt
     9121 ttgtgttaat tcatattgaa caagctattg taaagaatta ttaaaaggca caaatgtagg
     9181 aataacaaat gttgtaggtt ttcctctagg tgcatgcaca acagctacaa aagcattcga
     9241 agtttctgaa gcaattaaag atggtgcaac agaaattgat atggtattaa atattggtgc
     9301 attaaaagac aaaaattatg aattagtttt agaagacatg aaagctgtaa aaaaagcagc
     9361 tggatcacat gttgttaaat gtattatgga aaattgttta ttaacaaaag aagaaatcat
     9421 gaaagcttgt gaaatagctg ttgaagctgg attagaattt gttaaaacat caacaggatt
     9481 ttcaaaatca ggtgcaacat ttgaagatgt taaactaatg aagtcagttg ttaaagacaa
     9541 tgctttagtt aaagcagctg gtggagttag aacatttgaa gatgctcaaa aaatgattga
     9601 agcaggagct gaccgcttag gaacaagtgg tggagtagct attattaaag gtgaagaaaa
     9661 caacgcgagt tactaaaact agcgtttttt tattttgctc atttttatta aaagtttgca
     9721 aaaaggaaca taaaaattct aattattgat actaaagtta ttaaaaagaa gattttggtt
     9781 gattttataa aggtcataga atataatatt ttagcatgtg tattttgtgt gctcatttac
     9841 aaccgtctcc ggtgtttcgt cctttccaca agatatataa agccaagaaa tcgaaatact
     9901 ttcaagttac ggtaagcata tgatagtcca ttttaaaaca taattttaaa actgcaaact
     9961 acccaagaaa ttattacttt ctacgtcacg tattttgtac taatatcttt gtgtttacag
    10021 tcaaattaat tctaattatc tctctaacag ccttgtatcg tatatgcaaa tatgaaggaa
    10081 tcatgggaaa taggccctcc cacaatttta aaagaaaagg ggggattggg gggtacagtg
    10141 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
    10201 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc
    10261 gccggctcga gtggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
    10321 gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta
    10381 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
    10441 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
    10501 caggtgtcgt gacgcgggat ccgccaccat ggattacaaa gacgatgacg ataaggccac
    10561 catggaacaa aaacttattt ctgaagaaga tctgaaaagg ccggcggcca cgaaaaaggc
    10621 cggccaggca aaaaagaaaa agggaggttc cgctagcgga ggttcgatga gttcaaagac
    10681 tgggcctgtc gccgtcgatc caaccctgcg ccgccggatt gaacctcacg agtttgaagt
    10741 gttctttgac ccccgggagc tgagaaagga gacatgcctg ctgtacgaga tcaactgggg
    10801 aggcaggcac tccatctgga ggcacacctc tcagaacaca aataagcacg tggaggtgaa
    10861 cttcatcgag aagtttacca cagagcggta cttctgcccc ggcgcgccgg gaggtggtgg
    10921 cagcggtgga ggaggttctg ggggcggtgg ctcaatttta tggcatgaga tgtggcatga
    10981 gggtttggaa gaggcatcta gattgtattt cggtgaaaga aatgtcaagg gaatgttcga
    11041 ag
//
