LOCUS       sequence-34807-e         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Koushik SV, Blank PS, Vogel SS
  TITLE     Anomalous surplus energy transfer observed with multiple FRET 
            acceptors.
  JOURNAL   PLoS One. 2009 Nov 25;4(11):e8031. doi: 
            10.1371/journal.pone.0008031.
  PUBMED    19946626
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-11-25"; volume: "4(11):e8031. doi"; pages: " 
            10.1371/journal.pone.0008031"
COMMENT     Sequence Label: VCAA
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(99..809,810..812))
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGCGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(141..162)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     490..509
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     749..770
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 acagtaactc tacaatgtgg tatggctgat tatgatcagt tatctagatc cggtggatcc
       61 actagtaacg gccgccagtg tgctggaatt cgcccttcct tgtacagctc gtccatgccg
      121 agagtgatcc cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg
      181 gggtctttgc tcagcttgga ctggtagctc aggtagtggt tgtcgggcag cagcacgggg
      241 ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga gctgcacgcc gccgtcctcg
      301 atgttgtggc ggatcttgaa gttggccttg atgccgttct tctgcttgtc ggcggtgata
      361 tagacgttgt ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc
      421 ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc
      481 tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag
      541 ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcgggcg
      601 aagcactgca ggccgcagcc gagggtggtc acgagggtgg gccagggcac gggcagcttg
      661 ccggtggtgc agatgagctt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg
      721 ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc
      781 accccggtga acagctcctc gcccttgctc acccgtcgac tgcaa
//
