LOCUS       sequence-348116-        9934 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9934)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3155
FEATURES             Location/Qualifiers
     source          1..9934
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2688..2712)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2720..2761
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2872..3460
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2925..2945)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3343..3354)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3532..3765
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3843..3964
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3880..3899)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3934..3953
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4004..4139
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4159..4178)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4160..4178)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4188..4205)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4188..4204)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4197..4219)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4346..4801
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4433..4452)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4643..4664
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4827..4931
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4932..5000,5001..5792)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5150..5169)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5963..6551
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6452..6471
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6705..6722
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6839..6860
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6875..6880,6881..6898,6899..6905)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6913..6929
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6918..6940
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6937..6953
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6937..6953
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6972..6992
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6974..6992
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7020..7246
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7247..7427
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7474..7599
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8092..8325
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8510..8554
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8785..8995
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8807..9003
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8854..8989
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9035..9637
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9035..9054)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9531..9551
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9680..9797
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcatgttcgc agccgggctg gctcccttct acgcctccaa
     1261 cttcagcctc tggtcggccg cttactgctc ctcggccggc ccaggcggct gctccttccc
     1321 cttggacccc gccgccgtca aaaagccctc cttctgcatc gcagacattc tgcacgccgg
     1381 cgtgggggat ctgggggcgg ccccggaggg cctggcaggg gcctcggccg ccgccctcac
     1441 cgcgcacttg ggctcggttc acccgcacgc ctctttccaa gcggcggcca gatccccgct
     1501 tcgacccacc ccagtggtgg cgccctccga agtcccggct ggcttcccgc agcggctgtc
     1561 tccgctctca gccgcctacc accaccatca cccgcaacaa caacagcagc agcaacagcc
     1621 gcagcagcaa cagcctccgc ctccgccccg ggctggcgcc ctgcagcccc cggcctcggg
     1681 gacgcgagtg gttccgaacc cccaccacag tggctctgcc ccggccccct ccagcaaaga
     1741 cctcaaattt ggaattgacc gcattttatc tgcagaattt gacccaaaag tcaaagaagg
     1801 caacacgctg agagatctca cttccctgct aaccggtggg cggcccgccg gggtgcacct
     1861 ctcaggcctg cagccctcgg ccggccagtt cttcgcatct ctagatccca ttaacgaggc
     1921 ttctgcaatc ctgagtccct taaactcgaa cccaagaaat tcagttcagc atcagttcca
     1981 agacacgttt ccaggtccct atgctgtgct cacgaaggac accatgccgc agacgtacaa
     2041 aaggaagcgt tcatggtcgc gcgctgtgtt ctccaacctg cagaggaaag gcctggagaa
     2101 aaggtttgag attcagaagt acgtgaccaa gccggaccga aagcagctgg cggcgatgct
     2161 gggcctcacg gacgcacagg tgaaggtgtg gttccagaac cggcggatga agtggcggca
     2221 ctccaaggag gcccaggccc aaaaggacaa ggacaaggag gctggcgaga agccatcagg
     2281 tggagccccg gctgcggatg gcgagcagga cgagaggagc cccagccgtt ctgaaggcga
     2341 ggctgagagc gagagcagcg actccgagtc cctggacatg gcccccagcg acacggagcg
     2401 gactgagggg agtgagcgtt ctctgcacca aacaacagtt attaaggccc cggtcactgg
     2461 cgccctcatt accgccagca gtgctgggag tggtgggagc agcggcggcg gcggcaatag
     2521 tttcagcttc agcagcgcca gcagtcttag tagcagcagc accagtgcgg gttgcgccag
     2581 cagccttggc ggcggcggcg cctcggagct tctccctgca acacagccca cagccagcag
     2641 cgctcccaaa agccccgagc cagcccaagg cgcgcttggc tgcttatacc caactttctt
     2701 gtacaaagtg gttgatatcg gtaagcctat ccctaaccct ctcctcggtc tcgattctac
     2761 gtagtaatga actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
     2821 tggaaaggac gaagtttgga gaatactcca tggcacacgc gttaagtcga caatcaacct
     2881 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     2941 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     3001 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     3061 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     3121 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     3181 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     3241 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     3301 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     3361 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     3421 cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga
     3481 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     3541 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     3601 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     3661 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     3721 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt
     3781 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     3841 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     3901 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     3961 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     4021 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     4081 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     4141 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     4201 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     4261 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     4321 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     4381 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     4441 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     4501 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     4561 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     4621 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     4681 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     4741 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     4801 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     4861 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     4921 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     4981 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     5041 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     5101 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     5161 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     5221 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     5281 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     5341 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     5401 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     5461 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     5521 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     5581 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     5641 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     5701 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     5761 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     5821 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     5881 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     5941 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     6001 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     6061 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     6121 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     6181 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     6241 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6301 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     6361 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     6421 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     6481 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     6541 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     6601 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     6661 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     6721 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     6781 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     6841 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     6901 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     6961 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     7021 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     7081 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     7141 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     7201 gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt
     7261 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     7321 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     7381 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     7441 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     7501 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     7561 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     7621 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     7681 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     7741 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     7801 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     7861 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     7921 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     7981 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     8041 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     8101 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     8161 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     8221 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     8281 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     8341 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     8401 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     8461 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     8521 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     8581 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     8641 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     8701 gacccacctc ccaaccccga ggggacccat gcattgcatc tcaattagtc agcaaccagg
     8761 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgcg tctcaattag
     8821 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     8881 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     8941 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     9001 aaaaagcttt ctagaggtac caccggagct taccatgacc gagtacaagc ccacggtgcg
     9061 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     9121 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     9181 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     9241 cggcgccgcc gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     9301 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     9361 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     9421 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     9481 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     9541 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     9601 cacctggtgc atgacccgca agcccggtgc cagttaacga attctagatc ttgagacaaa
     9661 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
     9721 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     9781 caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt tggcgccggc
     9841 tcgaggcctg caggtgcaaa gatggataaa gttttaaaca gagaggaatc tttgcagcta
     9901 atggaccttc taggtcttga aaggagtggg aatt
//
