LOCUS       sequence-348118-       10000 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10000)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3156
FEATURES             Location/Qualifiers
     source          1..10000
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2754..2778)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2786..2827
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2938..3526
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2991..3011)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3409..3420)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3598..3831
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3909..4030
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3946..3965)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4000..4019
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4070..4205
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4225..4244)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4226..4244)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4254..4271)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4254..4270)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4263..4285)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4412..4867
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4499..4518)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4709..4730
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4893..4997
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4998..5066,5067..5858)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5216..5235)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6029..6617
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6518..6537
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6771..6788
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6905..6926
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6941..6946,6947..6964,6965..6971)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6979..6995
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6984..7006
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7003..7019
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7003..7019
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7038..7058
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7040..7058
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7086..7312
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7313..7493
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7540..7665
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8158..8391
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8576..8620
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8851..9061
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8873..9069
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8920..9055
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9101..9703
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9101..9120)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9597..9617
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9746..9863
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcatggacgg gttttatgat cagcaagtcc cttttatggt
     1261 cccagggaaa tctcgatctg aggaatgcag agggcggcct gtgattgaca gaaagaggaa
     1321 gtttttggac acagatctgg ctcacgattc tgaagagcta tttcaggatc tcagtcaact
     1381 tcaagaggct tggttagctg aagcacaagt tcctgatgat gaacagtttg tcccagattt
     1441 tcagtctgat aacctggtgc ttcatgcccc acctccaacc aagatcaaac gggagctgca
     1501 cagcccctcc tctgagctgt cgtcttgtag ccatgagcag gctcttggtg ctaactatgg
     1561 agaaaagtgc ctctacaact attgtgccta tgataggaag cctccctctg ggttcaagcc
     1621 attaacccct cctacaaccc ccctctcacc cacccatcag aatcccctat ttcccccacc
     1681 tcaggcaact ctgcccacct cagggcatgc ccctgcagct ggcccagttc aaggtgtggg
     1741 ccccgccccc gccccccatt cgcttccaga gcctggacca cagcagcaaa catttgcggt
     1801 cccccgacca ccacatcagc ccctgcagat gccaaagatg atgcctgaaa accagtatcc
     1861 atcagaacag agatttcaga gacaactgtc tgaaccctgc caccccttcc ctcctcagcc
     1921 aggagttcct ggagataatc gccccagtta ccatcggcaa atgtcagaac ctattgtccc
     1981 tgcagctccc ccgccccctc agggattcaa acaagaatac catgacccac tctatgaaca
     2041 tggggtcccg ggcatgccag ggcccccagc acacgggttc cagtcaccaa tgggaatcaa
     2101 gcaggagcct cgggattact gcgtcgattc agaagtgcct aactgccagt catcctacat
     2161 gagagggggt tatttctcca gcagccatga aggtttttca tatgaaaaag atccccgatt
     2221 atactttgac gacacttgtg ttgtgcctga gagactggaa ggcaaagtca aacaggagcc
     2281 taccatgtat cgagaggggc ccccttacca gaggcgaggt tcccttcagc tgtggcagtt
     2341 cctggtcacc cttcttgatg acccagccaa tgcccacttc attgcctgga caggtcgagg
     2401 catggagttc aagctgatag aaccggaaga ggttgctcgg cgctggggca tccagaagaa
     2461 ccggccagcc atgaactatg acaagctgag ccgctctctc cgctattact atgaaaaggg
     2521 catcatgcag aaggtggctg gagagcgata cgtctacaaa tttgtctgtg acccagatgc
     2581 cctcttctcc atggctttcc cggataacca gcgtccgttc ctgaaggcag agtccgagtg
     2641 ccacctcagc gaggaggaca ccctgccgct gacccacttt gaagacagcc ccgcttacct
     2701 cctggacatg gaccgctgca gcagcctccc ctatgccgaa ggctttgctt actacccaac
     2761 tttcttgtac aaagtggttg atatcggtaa gcctatccct aaccctctcc tcggtctcga
     2821 ttctacgtag taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat
     2881 atcttgtgga aaggacgatt cgagcctgtg gtcagaatca ctacgcgtta agtcgacaat
     2941 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     3001 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     3061 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     3121 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     3181 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     3241 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     3301 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     3361 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     3421 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     3481 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac
     3541 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     3601 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     3661 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     3721 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     3781 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata
     3841 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     3901 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     3961 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     4021 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     4081 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     4141 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     4201 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     4261 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     4321 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     4381 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     4441 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4501 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4561 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4621 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4681 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     4741 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     4801 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     4861 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     4921 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     4981 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     5041 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     5101 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     5161 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     5221 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     5281 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     5341 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     5401 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     5461 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     5521 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     5581 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     5641 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     5701 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     5761 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     5821 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     5881 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     5941 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     6001 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     6061 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     6121 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     6181 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     6241 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     6301 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     6361 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     6421 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     6481 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     6541 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     6601 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     6661 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     6721 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     6781 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     6841 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     6901 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     6961 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     7021 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
     7081 cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
     7141 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
     7201 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
     7261 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
     7321 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     7381 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     7441 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     7501 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
     7561 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
     7621 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
     7681 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     7741 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
     7801 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
     7861 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
     7921 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
     7981 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
     8041 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     8101 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     8161 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     8221 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     8281 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     8341 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     8401 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     8461 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     8521 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     8581 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     8641 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     8701 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     8761 gtttcagacc cacctcccaa ccccgagggg acccatgcat tgcatctcaa ttagtcagca
     8821 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcgtctc
     8881 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     8941 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     9001 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     9061 ttttgcaaaa agctttctag aggtaccacc ggagcttacc atgaccgagt acaagcccac
     9121 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     9181 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     9241 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     9301 ggacgacggc gccgccgtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     9361 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     9421 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     9481 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     9541 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     9601 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     9661 accgcgcacc tggtgcatga cccgcaagcc cggtgccagt taacgaattc tagatcttga
     9721 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     9781 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     9841 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc
     9901 gccggctcga ggcctgcagg tgcaaagatg gataaagttt taaacagaga ggaatctttg
     9961 cagctaatgg accttctagg tcttgaaagg agtgggaatt
//
