LOCUS       sequence-348142-        4784 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Xenopus oocyte expression vector containing YGL084C. Contains T7 RNA
            polymerase binding site, and adds polyA tail to YGL084C. Used for 
            generation of mRNA, for injection into xenopus laevis oocytes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Moller-Hansen I, Saez-Saez J, van der Hoek SA, Dyekjaer JD, 
            Christensen HB, Wright Muelas M, O'Hagan S, Kell DB, Borodina I
  TITLE     Deorphanizing solute carriers in Saccharomyces cerevisiae for 
            secondary uptake of xenobiotic compounds.
  JOURNAL   Front Microbiol. 2024 Apr 12;15:1376653. doi: 
            10.3389/fmicb.2024.1376653.
  PUBMED    38680917
REFERENCE   2  (bases 1 to 4784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2024-04-12"; volume: "15:1376653. doi"; pages: "
            10.3389/fmicb.2024.1376653"
COMMENT     Sequence Label: pCfB8408
FEATURES             Location/Qualifiers
     source          1..4784
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     76..98
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     90..107
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     91..107
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     114..133
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        114..132
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           164..206
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     3'UTR           2023..2148
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus 
                     laevis"
     promoter        complement(2239..2257)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2240..2257)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2275..2291)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2275..2291)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2288..2310)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2299..2315
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2323..2329,2330..2347,2348..2353))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2368..2389
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2506..2523)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2677..3265)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2757..2776)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3436..4227,4228..4296))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4059..4078
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4297..4401)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4469..4487
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4525..4547)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4647..4666
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
       61 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg
      121 actcactata gggcgaatta attcgagctc ggtacccagc ttgcttgttc tttttgcaga
      181 agctcagaat aaacgctcaa ctttggcaga tcaattcccg gggctgaggc ttaatatgtc
      241 gctgatcagc atcctgtctc ccctaattac ttccgagggc ttagattcaa gaatcaaacc
      301 ttcaccaaaa aaggatgcct ctactaccac taagccatca ctatggaaaa ctactgagtt
      361 caaattctac tacattgcat ttctggtcgt ggttcccttg atgttctatg ctgggttaca
      421 agctagttcg cccgaaaatc caaactatgc aagatacgaa cgtctcctat ctcaaggttg
      481 gttatttggc agaaaagtag acaatagtga ttctcaatat aggtttttca gggacaattt
      541 tgcgctattg tcagttttaa tgctagtcca cacttctata aaacgcattg tactttattc
      601 aacaaatatc actaaattga ggtttgatct gatatttggt ttgatctttt tagtggccgc
      661 tcatggtgtc aattcgataa gaattttagc ccatatgcta attttatatg ccatcgccca
      721 tgtactaaag aactttagaa gaatagccac catcagcatt tggatttatg gtatttctac
      781 gctttttatt aacgacaact tcagagcata tccatttggt aatatttgct cttttttaag
      841 cccattggat cattggtata gaggtatcat tccaagatgg gatgtctttt tcaattttac
      901 tcttttgaga gtcttaagtt acaacttgga cttcttagag aggtgggaga atttacaaaa
      961 gaagaaaagt ccatcctatg aatcaaaaga agctaaatca gccattttgc tcaatgaacg
     1021 tgctagatta actgctgcac accccataca ggactacagc ttaatgaatt atattgcata
     1081 tgttacttac acgccacttt tcattgccgg ccccattata acattcaatg attatgttta
     1141 ccaatcgaaa cataccttgc catcaataaa tttcaaattc attttttact atgcggtgag
     1201 attcgttatt gctctcttat ctatggagtt cattttacac tttctctacg ttgtggcaat
     1261 ctcaaaaacc aaagcgtggg aaaatgacac acctttccag atttccatga ttggcttatt
     1321 taatttgaat attatttggc taaaactact gattccgtgg aggctgttta ggctgtgggc
     1381 tttgctagac ggaatcgata cacctgaaaa tatgatcagg tgtgttgata acaattacag
     1441 ttcactagca ttctggagag cttggcatag gagctacaat aagtgggttg tccgttacat
     1501 atatattcct ctaggtggct caaaaaatag agttttgaca tcactagcag tcttttcctt
     1561 cgtagctata tggcatgaca tcgaactaaa gttattatta tggggttggc taatagtttt
     1621 gttcctctta ccagaaattt ttgctaccca aattttctct cattataccg acgcagtctg
     1681 gtacagacac gtttgcgctg tcggtgctgt tttcaacata tgggttatga tgatcgctaa
     1741 tctttttgga ttctgcttgg gctctgacgg tactaaaaaa ttactaagcg atatgttctg
     1801 taccgtatct ggtttcaaat ttgtaagttt ggcaagcgtt agtttattca tcgcagtaca
     1861 aataatgttt gaaatcagag aagaagaaaa gaggcacgga atttacctaa aatgctgaat
     1921 taaacctcag cgaattcgtg agaaagcttg ggaataggcc tttcagatct gcccgagacc
     1981 atggatgaag tatctagaaa tagcttgatc tggttaccac taaaccagcc tcaagaacac
     2041 ccgaatggag tctctaagct acataatacc aacttacact ttacaaaatg ttgtccccca
     2101 aaatgtagcc attcgtatct gctcctaawa aaaaraaagt ttcttcacat tctaaaaaaa
     2161 aaaaaaaaaa aaaaaaaaaa aaaaaaamcc cccccccccc ccccctgcag gcggccgcct
     2221 cgaggctagc ttgagtattc tatagtgtca cctaaatagc ttggcgtaat catggtcata
     2281 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     2341 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     2401 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     2461 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     2521 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     2581 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     2641 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     2701 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     2761 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     2821 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     2881 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     2941 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     3001 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     3061 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     3121 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     3181 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     3241 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     3301 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     3361 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     3421 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     3481 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     3541 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     3601 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     3661 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     3721 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     3781 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     3841 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     3901 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     3961 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     4021 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     4081 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     4141 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     4201 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     4261 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     4321 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     4381 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     4441 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
     4501 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     4561 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     4621 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
     4681 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
     4741 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cggg
//
