LOCUS       sequence-348147-        4655 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Xenopus oocyte expression vector containing YAL022C. Contains T7 RNA
            polymerase binding site, and adds polyA tail to YAL022C. Used for 
            generation of mRNA, for injection into xenopus laevis oocytes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Moller-Hansen I, Saez-Saez J, van der Hoek SA, Dyekjaer JD, 
            Christensen HB, Wright Muelas M, O'Hagan S, Kell DB, Borodina I
  TITLE     Deorphanizing solute carriers in Saccharomyces cerevisiae for 
            secondary uptake of xenobiotic compounds.
  JOURNAL   Front Microbiol. 2024 Apr 12;15:1376653. doi: 
            10.3389/fmicb.2024.1376653.
  PUBMED    38680917
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2024-04-12"; volume: "15:1376653. doi"; pages: "
            10.3389/fmicb.2024.1376653"
COMMENT     Sequence Label: pCfB8150
FEATURES             Location/Qualifiers
     source          1..4655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     3'UTR           1688..1813
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus 
                     laevis"
     promoter        complement(1904..1922)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1905..1922)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1940..1956)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1940..1956)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1953..1975)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1964..1980
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1988..1994,1995..2012,2013..2018))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2033..2054
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2171..2188)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2342..2930)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2422..2441)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3101..3892,3893..3961))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3724..3743
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3962..4066)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4134..4152
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4190..4212)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4312..4331
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4525..4547
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4539..4556
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4540..4556
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4563..4582
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4563..4581
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           4613..4655
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
ORIGIN
        1 cagatcaatt cccggggctg aggcttaata tgagtactag tgcggacact gataccatca
       61 agaagccaat ccttgcggtg ccagagcctg cactggccga tacgcattca gaggagatat
      121 cacgctctgg agaagaacat gaatcagaga acaacgagca ctcagatgaa gaaggcgata
      181 attattctga aagagagcaa tctgtgtcaa ccgaaccact ggatacattg ccgttaagaa
      241 aaaagttgaa aaatctttca tatattacat ttttcgccat aggaataggt cttttatggc
      301 cgtggaactg tatcctcagt gcctcgcaat attttaagca cgatattttc aaagacacct
      361 ccatttgggc aaagatcttc acaagctcta tgatgtcctt ttctaccata tcgtcaatgc
      421 tgttcaacat ctacttggcc aaaagacagt acaaatactc gagaagggtc ataaacgggc
      481 ttgtgtggga gattattgtc tttactgtca tgtgcttctt tacaattttg catttccttt
      541 tacctaaatg gttcaatttc atgtttataa tgatgcttgt agtgatcagt tccatgggga
      601 cagccatgac acagaatggt atcatggcca tagccaacgt cttcggttcc gagtacagtc
      661 aaggtgtcat ggtggggcaa gccgttgctg gtgtcctgcc ctccctagtg ctttttgccc
      721 tagctttcat cgagaactct tctgtgtcta ctacgggcgg gattcttcta tactttttta
      781 ccacaacact cgtggtcacc atttgtgtgg tcatgttcag cgtgagcaaa atcagtcgga
      841 aagtgaacga gaattggaat gtggaagacg gacatatcac tgatgtgttg ttagggtctc
      901 tgcgctccaa tgaggaggaa atccgtattg ttggccgcat tgaccaaatg gaggatgaag
      961 accaccgccg caccaacggc actcgcgacg acaacgacga aggtgaggaa ctccaactaa
     1021 aagtgccttt cgaggtctta tttgccaaac taaagtacct ggttctttcc atattcacca
     1081 cgtttgtcgt aactcttgtt tttcctgtat ttgcgtctgc cacctacgtg acagggcttc
     1141 ctttaagcaa cgcacagtac atacctctca tattcacgct gtggaaccta ggcgaccttt
     1201 acggaagagt cattgccgac tggcccatgt tccgtgacca gaaatttacg ccacgcaaaa
     1261 ccttcatcta ctcattgttg cgggtggccg caataccact gttcttgatg ttcacagcca
     1321 tcacctcctc ttcaagcggc gatgaagagc acaatggctc cgtaatcgtt gacttgtgtt
     1381 acatgctact gcagttcctt ttcggcgtaa ccaacgggca cgtcatctct atgagtttca
     1441 tgaaggtgcc agagcaactg gacaacgacg acgagaagga agcggccggt ggattcacta
     1501 atattttcgt ttctacagga ctagctctgg gcagcatcat aagctacgta ttcgtcttca
     1561 taattgactt tattatcagg tgaattaaac ctcagcgaat tcgtgagaaa gcttgggaat
     1621 aggcctttca gatctgcccg agaccatgga tgaagtatct agaaatagct tgatctggtt
     1681 accactaaac cagcctcaag aacacccgaa tggagtctct aagctacata ataccaactt
     1741 acactttaca aaatgttgtc ccccaaaatg tagccattcg tatctgctcc taawaaaaar
     1801 aaagtttctt cacattctaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aamccccccc
     1861 cccccccccc tgcaggcggc cgcctcgagg ctagcttgag tattctatag tgtcacctaa
     1921 atagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     1981 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     2041 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     2101 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     2161 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     2221 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     2281 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     2341 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     2401 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     2461 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     2521 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     2581 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     2641 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     2701 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     2761 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     2821 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     2881 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     2941 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     3001 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     3061 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     3121 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     3181 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     3241 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     3301 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     3361 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     3421 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     3481 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     3541 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     3601 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     3661 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     3721 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     3781 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     3841 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     3901 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     3961 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     4021 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     4081 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     4141 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     4201 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     4261 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     4321 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     4381 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     4441 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     4501 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat
     4561 tgtaatacga ctcactatag ggcgaattaa ttcgagctcg gtacccagct tgcttgttct
     4621 ttttgcagaa gctcagaata aacgctcaac tttgg
//
